ASSOCIATION OF INTERLEUKIN-1α GENE POLYMORPHISM (ENCODED REGION rs1800587 5`UTR) WITH EFFICIENCY OF IN VITRO FERTILIZATION

Due to quite low efficiency and high costs of in vitro fertilization protocols, there is a necessity for development of new diagnostic criteria for selection of patients. The purpose of this study was complex analysis of association of polymorphic variants of IL-1α gene (rs1800587 5`UTR region) and...

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Autores principales:	A. V. Lapshtaeva, I. V. Sychev, L. N. Goncharova
Formato:	article
Lenguaje:	RU
Publicado:	SPb RAACI 2018
Materias:	gene polymorphism il-1α tubo-peritoneal infertility in vitro fertilization Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/b80fb10b2bf3467489de3280d379062c
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spelling	oai:doaj.org-article:b80fb10b2bf3467489de3280d379062c2021-11-18T08:03:47ZASSOCIATION OF INTERLEUKIN-1α GENE POLYMORPHISM (ENCODED REGION rs1800587 5`UTR) WITH EFFICIENCY OF IN VITRO FERTILIZATION1563-06252313-741X10.15789/1563-0625-2018-3-417-424https://doaj.org/article/b80fb10b2bf3467489de3280d379062c2018-06-01T00:00:00Zhttps://www.mimmun.ru/mimmun/article/view/1532https://doaj.org/toc/1563-0625https://doaj.org/toc/2313-741XDue to quite low efficiency and high costs of in vitro fertilization protocols, there is a necessity for development of new diagnostic criteria for selection of patients. The purpose of this study was complex analysis of association of polymorphic variants of IL-1α gene (rs1800587 5`UTR region) and main diagnostic criteria of women with tubo-peritoneal infertility and success rate of in vitro fertilization procedure. We have examined 120 women with tubo-peritoneal infertility who were subjected to the microflare protocol of controlled ovarian hyperstimulation which was followed by embryo transfer. Concentration of IL-1α in blood serum was defined by ELISA method by means of test systems of the "Cytokine LLC" (St. Petersburg, Russian Federation). Polymorphic marker rs1800587 at 5`UTR region was analysed with PCR method followed by the sequencing. We have detected no particular features in distribution of polymorphic IL-1α gene variants in pregnant women. The T/T genotype seems to be a conditionally favorable predictor for pregnancy resulting from in vitro fertilization. It has been shown that the IL-1α content in blood serum of pregnant women was increased as compared to women who did not become pregnant. It was also shown that pregnant women with T/T genotype had higher serum IL-1α levels than women with other genotypes. During transvaginal puncture, we have detected that pregnant women with T/T genotype had more oocytes than women with C/C genotype. We have not found significant differences in 17-oxyprogesterone, estradiol, follicle-stimulating hormone, testosterone contents for groups of women with different IL-1α genotypes. As based on this study, the IL-1a involvement in the implantation become more evident. Further studies are required concerning influence of IL-1a on folliculogenesis, ovulation, implantation, decidualization and placenta formation.A. V. LapshtaevaI. V. SychevL. N. GoncharovaSPb RAACIarticlegene polymorphismil-1αtubo-peritoneal infertilityin vitro fertilizationImmunologic diseases. AllergyRC581-607RUMedicinskaâ Immunologiâ, Vol 20, Iss 3, Pp 417-424 (2018)
institution	DOAJ
collection	DOAJ
language	RU
topic	gene polymorphism il-1α tubo-peritoneal infertility in vitro fertilization Immunologic diseases. Allergy RC581-607
spellingShingle	gene polymorphism il-1α tubo-peritoneal infertility in vitro fertilization Immunologic diseases. Allergy RC581-607 A. V. Lapshtaeva I. V. Sychev L. N. Goncharova ASSOCIATION OF INTERLEUKIN-1α GENE POLYMORPHISM (ENCODED REGION rs1800587 5`UTR) WITH EFFICIENCY OF IN VITRO FERTILIZATION
description	Due to quite low efficiency and high costs of in vitro fertilization protocols, there is a necessity for development of new diagnostic criteria for selection of patients. The purpose of this study was complex analysis of association of polymorphic variants of IL-1α gene (rs1800587 5`UTR region) and main diagnostic criteria of women with tubo-peritoneal infertility and success rate of in vitro fertilization procedure. We have examined 120 women with tubo-peritoneal infertility who were subjected to the microflare protocol of controlled ovarian hyperstimulation which was followed by embryo transfer. Concentration of IL-1α in blood serum was defined by ELISA method by means of test systems of the "Cytokine LLC" (St. Petersburg, Russian Federation). Polymorphic marker rs1800587 at 5`UTR region was analysed with PCR method followed by the sequencing. We have detected no particular features in distribution of polymorphic IL-1α gene variants in pregnant women. The T/T genotype seems to be a conditionally favorable predictor for pregnancy resulting from in vitro fertilization. It has been shown that the IL-1α content in blood serum of pregnant women was increased as compared to women who did not become pregnant. It was also shown that pregnant women with T/T genotype had higher serum IL-1α levels than women with other genotypes. During transvaginal puncture, we have detected that pregnant women with T/T genotype had more oocytes than women with C/C genotype. We have not found significant differences in 17-oxyprogesterone, estradiol, follicle-stimulating hormone, testosterone contents for groups of women with different IL-1α genotypes. As based on this study, the IL-1a involvement in the implantation become more evident. Further studies are required concerning influence of IL-1a on folliculogenesis, ovulation, implantation, decidualization and placenta formation.
format	article
author	A. V. Lapshtaeva I. V. Sychev L. N. Goncharova
author_facet	A. V. Lapshtaeva I. V. Sychev L. N. Goncharova
author_sort	A. V. Lapshtaeva
title	ASSOCIATION OF INTERLEUKIN-1α GENE POLYMORPHISM (ENCODED REGION rs1800587 5`UTR) WITH EFFICIENCY OF IN VITRO FERTILIZATION
title_short	ASSOCIATION OF INTERLEUKIN-1α GENE POLYMORPHISM (ENCODED REGION rs1800587 5`UTR) WITH EFFICIENCY OF IN VITRO FERTILIZATION
title_full	ASSOCIATION OF INTERLEUKIN-1α GENE POLYMORPHISM (ENCODED REGION rs1800587 5`UTR) WITH EFFICIENCY OF IN VITRO FERTILIZATION
title_fullStr	ASSOCIATION OF INTERLEUKIN-1α GENE POLYMORPHISM (ENCODED REGION rs1800587 5`UTR) WITH EFFICIENCY OF IN VITRO FERTILIZATION
title_full_unstemmed	ASSOCIATION OF INTERLEUKIN-1α GENE POLYMORPHISM (ENCODED REGION rs1800587 5`UTR) WITH EFFICIENCY OF IN VITRO FERTILIZATION
title_sort	association of interleukin-1α gene polymorphism (encoded region rs1800587 5`utr) with efficiency of in vitro fertilization
publisher	SPb RAACI
publishDate	2018
url	https://doaj.org/article/b80fb10b2bf3467489de3280d379062c
work_keys_str_mv	AT avlapshtaeva associationofinterleukin1agenepolymorphismencodedregionrs18005875utrwithefficiencyofinvitrofertilization AT ivsychev associationofinterleukin1agenepolymorphismencodedregionrs18005875utrwithefficiencyofinvitrofertilization AT lngoncharova associationofinterleukin1agenepolymorphismencodedregionrs18005875utrwithefficiencyofinvitrofertilization
_version_	1718422375123386368

ASSOCIATION OF INTERLEUKIN-1α GENE POLYMORPHISM (ENCODED REGION rs1800587 5`UTR) WITH EFFICIENCY OF IN VITRO FERTILIZATION

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