A large panel of chicken cells are invaded <italic toggle="yes">in vivo</italic> by <italic toggle="yes">Salmonella</italic> Typhimurium even when depleted of all known invasion factors

Poultry are the main source of human infection by Salmonella. As infected poultry are asymptomatic, identifying infected poultry farms is difficult, thus controlling animal infections is of primary importance. As cell tropism is known to govern disease, our aim was therefore to identify infected hos...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	S. M. Roche, S. Holbert, Y. Le Vern, C. Rossignol, A. Rossignol, P. Velge, I. Virlogeux-Payant
Formato:	article
Lenguaje:	EN
Publicado:	The Royal Society 2021
Materias:	Salmonella poultry host–pathogen interaction invasion gall bladder Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/b813dbe696b0465cac609f3aaeb5f015
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:b813dbe696b0465cac609f3aaeb5f015
record_format	dspace
spelling	oai:doaj.org-article:b813dbe696b0465cac609f3aaeb5f0152021-11-17T08:06:16ZA large panel of chicken cells are invaded <italic toggle="yes">in vivo</italic> by <italic toggle="yes">Salmonella</italic> Typhimurium even when depleted of all known invasion factors10.1098/rsob.2101172046-2441https://doaj.org/article/b813dbe696b0465cac609f3aaeb5f0152021-11-01T00:00:00Zhttps://royalsocietypublishing.org/doi/10.1098/rsob.210117https://doaj.org/toc/2046-2441Poultry are the main source of human infection by Salmonella. As infected poultry are asymptomatic, identifying infected poultry farms is difficult, thus controlling animal infections is of primary importance. As cell tropism is known to govern disease, our aim was therefore to identify infected host–cell types in the organs of chicks known to be involved in Salmonella infection and investigate the role of the three known invasion factors in this process (T3SS-1, Rck and PagN). Chicks were inoculated with wild-type or isogenic fluorescent Salmonella Typhimurium mutants via the intracoelomic route. Our results show that liver, spleen, gall bladder and aortic vessels could be foci of infection, and that phagocytic and non-phagocytic cells, including immune, epithelial and endothelial cells, are invaded in vivo in each organ. Moreover, a mutant defective for the T3SS-1, Rck and PagN remained able to colonize organs like the wild-type strain and invaded non-phagocytic cells in each organ studied. As the infection of the gall bladder had not previously been described in chicks, invasion of gall bladder cells was confirmed by immunohistochemistry and infection was shown to last several weeks after inoculation. Altogether, for the first time these findings provide insights into cell tropism of Salmonella in relevant organs involved in Salmonella infection in chicks and also demonstrate that the known invasion factors are not required for entry into these cell types.S. M. RocheS. HolbertY. Le VernC. RossignolA. RossignolP. VelgeI. Virlogeux-PayantThe Royal SocietyarticleSalmonellapoultryhost–pathogen interactioninvasiongall bladderBiology (General)QH301-705.5ENOpen Biology, Vol 11, Iss 11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Salmonella poultry host–pathogen interaction invasion gall bladder Biology (General) QH301-705.5
spellingShingle	Salmonella poultry host–pathogen interaction invasion gall bladder Biology (General) QH301-705.5 S. M. Roche S. Holbert Y. Le Vern C. Rossignol A. Rossignol P. Velge I. Virlogeux-Payant A large panel of chicken cells are invaded <italic toggle="yes">in vivo</italic> by <italic toggle="yes">Salmonella</italic> Typhimurium even when depleted of all known invasion factors
description	Poultry are the main source of human infection by Salmonella. As infected poultry are asymptomatic, identifying infected poultry farms is difficult, thus controlling animal infections is of primary importance. As cell tropism is known to govern disease, our aim was therefore to identify infected host–cell types in the organs of chicks known to be involved in Salmonella infection and investigate the role of the three known invasion factors in this process (T3SS-1, Rck and PagN). Chicks were inoculated with wild-type or isogenic fluorescent Salmonella Typhimurium mutants via the intracoelomic route. Our results show that liver, spleen, gall bladder and aortic vessels could be foci of infection, and that phagocytic and non-phagocytic cells, including immune, epithelial and endothelial cells, are invaded in vivo in each organ. Moreover, a mutant defective for the T3SS-1, Rck and PagN remained able to colonize organs like the wild-type strain and invaded non-phagocytic cells in each organ studied. As the infection of the gall bladder had not previously been described in chicks, invasion of gall bladder cells was confirmed by immunohistochemistry and infection was shown to last several weeks after inoculation. Altogether, for the first time these findings provide insights into cell tropism of Salmonella in relevant organs involved in Salmonella infection in chicks and also demonstrate that the known invasion factors are not required for entry into these cell types.
format	article
author	S. M. Roche S. Holbert Y. Le Vern C. Rossignol A. Rossignol P. Velge I. Virlogeux-Payant
author_facet	S. M. Roche S. Holbert Y. Le Vern C. Rossignol A. Rossignol P. Velge I. Virlogeux-Payant
author_sort	S. M. Roche
title	A large panel of chicken cells are invaded <italic toggle="yes">in vivo</italic> by <italic toggle="yes">Salmonella</italic> Typhimurium even when depleted of all known invasion factors
title_short	A large panel of chicken cells are invaded <italic toggle="yes">in vivo</italic> by <italic toggle="yes">Salmonella</italic> Typhimurium even when depleted of all known invasion factors
title_full	A large panel of chicken cells are invaded <italic toggle="yes">in vivo</italic> by <italic toggle="yes">Salmonella</italic> Typhimurium even when depleted of all known invasion factors
title_fullStr	A large panel of chicken cells are invaded <italic toggle="yes">in vivo</italic> by <italic toggle="yes">Salmonella</italic> Typhimurium even when depleted of all known invasion factors
title_full_unstemmed	A large panel of chicken cells are invaded <italic toggle="yes">in vivo</italic> by <italic toggle="yes">Salmonella</italic> Typhimurium even when depleted of all known invasion factors
title_sort	large panel of chicken cells are invaded <italic toggle="yes">in vivo</italic> by <italic toggle="yes">salmonella</italic> typhimurium even when depleted of all known invasion factors
publisher	The Royal Society
publishDate	2021
url	https://doaj.org/article/b813dbe696b0465cac609f3aaeb5f015
work_keys_str_mv	AT smroche alargepanelofchickencellsareinvadeditalictoggleyesinvivoitalicbyitalictoggleyessalmonellaitalictyphimuriumevenwhendepletedofallknowninvasionfactors AT sholbert alargepanelofchickencellsareinvadeditalictoggleyesinvivoitalicbyitalictoggleyessalmonellaitalictyphimuriumevenwhendepletedofallknowninvasionfactors AT ylevern alargepanelofchickencellsareinvadeditalictoggleyesinvivoitalicbyitalictoggleyessalmonellaitalictyphimuriumevenwhendepletedofallknowninvasionfactors AT crossignol alargepanelofchickencellsareinvadeditalictoggleyesinvivoitalicbyitalictoggleyessalmonellaitalictyphimuriumevenwhendepletedofallknowninvasionfactors AT arossignol alargepanelofchickencellsareinvadeditalictoggleyesinvivoitalicbyitalictoggleyessalmonellaitalictyphimuriumevenwhendepletedofallknowninvasionfactors AT pvelge alargepanelofchickencellsareinvadeditalictoggleyesinvivoitalicbyitalictoggleyessalmonellaitalictyphimuriumevenwhendepletedofallknowninvasionfactors AT ivirlogeuxpayant alargepanelofchickencellsareinvadeditalictoggleyesinvivoitalicbyitalictoggleyessalmonellaitalictyphimuriumevenwhendepletedofallknowninvasionfactors AT smroche largepanelofchickencellsareinvadeditalictoggleyesinvivoitalicbyitalictoggleyessalmonellaitalictyphimuriumevenwhendepletedofallknowninvasionfactors AT sholbert largepanelofchickencellsareinvadeditalictoggleyesinvivoitalicbyitalictoggleyessalmonellaitalictyphimuriumevenwhendepletedofallknowninvasionfactors AT ylevern largepanelofchickencellsareinvadeditalictoggleyesinvivoitalicbyitalictoggleyessalmonellaitalictyphimuriumevenwhendepletedofallknowninvasionfactors AT crossignol largepanelofchickencellsareinvadeditalictoggleyesinvivoitalicbyitalictoggleyessalmonellaitalictyphimuriumevenwhendepletedofallknowninvasionfactors AT arossignol largepanelofchickencellsareinvadeditalictoggleyesinvivoitalicbyitalictoggleyessalmonellaitalictyphimuriumevenwhendepletedofallknowninvasionfactors AT pvelge largepanelofchickencellsareinvadeditalictoggleyesinvivoitalicbyitalictoggleyessalmonellaitalictyphimuriumevenwhendepletedofallknowninvasionfactors AT ivirlogeuxpayant largepanelofchickencellsareinvadeditalictoggleyesinvivoitalicbyitalictoggleyessalmonellaitalictyphimuriumevenwhendepletedofallknowninvasionfactors
_version_	1718425821921673216

A large panel of chicken cells are invaded <italic toggle="yes">in vivo</italic> by <italic toggle="yes">Salmonella</italic> Typhimurium even when depleted of all known invasion factors

Ejemplares similares