The laboratory test procedure to confirm rotavirus vaccine infection in severe complex immunodeficiency patients

The rotavirus vaccine is a live vaccine, and there is a possibility of infection by the virus strain used in the vaccine. We investigated the process of determining whether an infection was caused by the vaccine strain in a severe complex immunodeficiency (SCID) patient with rotavirus infection. The...

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Autores principales:	Su-Jin Chae, Seung-Rye Cho, Wooyoung Choi, Myung-Guk Han, Deog-Yong Lee
Formato:	article
Lenguaje:	EN
Publicado:	Korea Centers for Disease Control & Prevention 2021
Materias:	infantile diarrhea laboratory test rotavirus infections rotavirus vaccines severe combined immunodeficiency Special situations and conditions RC952-1245 Infectious and parasitic diseases RC109-216
Acceso en línea:	https://doaj.org/article/b8223bfb2e024d58b2baa630e58b76fb
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spelling	oai:doaj.org-article:b8223bfb2e024d58b2baa630e58b76fb2021-11-05T00:06:00ZThe laboratory test procedure to confirm rotavirus vaccine infection in severe complex immunodeficiency patients2210-90992210-911010.24171/j.phrp.2021.0079https://doaj.org/article/b8223bfb2e024d58b2baa630e58b76fb2021-08-01T00:00:00Zhttp://ophrp.org/upload/pdf/j-phrp-2021-0079.pdfhttps://doaj.org/toc/2210-9099https://doaj.org/toc/2210-9110The rotavirus vaccine is a live vaccine, and there is a possibility of infection by the virus strain used in the vaccine. We investigated the process of determining whether an infection was caused by the vaccine strain in a severe complex immunodeficiency (SCID) patient with rotavirus infection. The patient was vaccinated with RotaTeq prior to being diagnosed with SCID. The testing process was conducted in the following order: confirming rotavirus infection, determining its genotype, and confirming the vaccine strain. Rotavirus infection was confirmed through enzyme immunoassay and VP6 gene detection. G1 and P[8] were identified by multiplex polymerase chain reaction for the genotype, and G3 was further identified using a single primer. By detecting the fingerprint gene (WC3) of RotaTeq, it was confirmed that the detected virus was the vaccine strain. Genotypes G1 and P[8] were identified, and the infection was suspected of having been caused by rotavirus G1P[8]. G1P[8] is the most commonly detected genotype worldwide and is not included in the recombinant strains used in vaccines. Therefore, the infection was confirmed to have been caused by the vaccine strain by analyzing the genetic relationship between VP4 and VP7. Rotavirus infection by the vaccine strain can be identified through genotyping and fingerprint gene detection. However, genetic linkage analysis will also help to identify vaccine strains.Su-Jin ChaeSeung-Rye ChoWooyoung ChoiMyung-Guk HanDeog-Yong LeeKorea Centers for Disease Control & Preventionarticleinfantile diarrhealaboratory testrotavirus infectionsrotavirus vaccinessevere combined immunodeficiencySpecial situations and conditionsRC952-1245Infectious and parasitic diseasesRC109-216ENOsong Public Health and Research Perspectives, Vol 12, Iss 4, Pp 269-273 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	infantile diarrhea laboratory test rotavirus infections rotavirus vaccines severe combined immunodeficiency Special situations and conditions RC952-1245 Infectious and parasitic diseases RC109-216
spellingShingle	infantile diarrhea laboratory test rotavirus infections rotavirus vaccines severe combined immunodeficiency Special situations and conditions RC952-1245 Infectious and parasitic diseases RC109-216 Su-Jin Chae Seung-Rye Cho Wooyoung Choi Myung-Guk Han Deog-Yong Lee The laboratory test procedure to confirm rotavirus vaccine infection in severe complex immunodeficiency patients
description	The rotavirus vaccine is a live vaccine, and there is a possibility of infection by the virus strain used in the vaccine. We investigated the process of determining whether an infection was caused by the vaccine strain in a severe complex immunodeficiency (SCID) patient with rotavirus infection. The patient was vaccinated with RotaTeq prior to being diagnosed with SCID. The testing process was conducted in the following order: confirming rotavirus infection, determining its genotype, and confirming the vaccine strain. Rotavirus infection was confirmed through enzyme immunoassay and VP6 gene detection. G1 and P[8] were identified by multiplex polymerase chain reaction for the genotype, and G3 was further identified using a single primer. By detecting the fingerprint gene (WC3) of RotaTeq, it was confirmed that the detected virus was the vaccine strain. Genotypes G1 and P[8] were identified, and the infection was suspected of having been caused by rotavirus G1P[8]. G1P[8] is the most commonly detected genotype worldwide and is not included in the recombinant strains used in vaccines. Therefore, the infection was confirmed to have been caused by the vaccine strain by analyzing the genetic relationship between VP4 and VP7. Rotavirus infection by the vaccine strain can be identified through genotyping and fingerprint gene detection. However, genetic linkage analysis will also help to identify vaccine strains.
format	article
author	Su-Jin Chae Seung-Rye Cho Wooyoung Choi Myung-Guk Han Deog-Yong Lee
author_facet	Su-Jin Chae Seung-Rye Cho Wooyoung Choi Myung-Guk Han Deog-Yong Lee
author_sort	Su-Jin Chae
title	The laboratory test procedure to confirm rotavirus vaccine infection in severe complex immunodeficiency patients
title_short	The laboratory test procedure to confirm rotavirus vaccine infection in severe complex immunodeficiency patients
title_full	The laboratory test procedure to confirm rotavirus vaccine infection in severe complex immunodeficiency patients
title_fullStr	The laboratory test procedure to confirm rotavirus vaccine infection in severe complex immunodeficiency patients
title_full_unstemmed	The laboratory test procedure to confirm rotavirus vaccine infection in severe complex immunodeficiency patients
title_sort	laboratory test procedure to confirm rotavirus vaccine infection in severe complex immunodeficiency patients
publisher	Korea Centers for Disease Control & Prevention
publishDate	2021
url	https://doaj.org/article/b8223bfb2e024d58b2baa630e58b76fb
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The laboratory test procedure to confirm rotavirus vaccine infection in severe complex immunodeficiency patients

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