Zyxin Is Involved in Fibroblast Rigidity Sensing and Durotaxis

Focal adhesions (FAs) are specialized structures that enable cells to sense their extracellular matrix rigidity and transmit these signals to the interior of the cells, bringing about actin cytoskeleton reorganization, FA maturation, and cell migration. It is known that cells migrate towards regions...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Ai Kia Yip, Songjing Zhang, Lor Huai Chong, Elsie Cheruba, Jessie Yong Xing Woon, Theng Xuan Chua, Corinna Jie Hui Goh, Haibo Yang, Chor Yong Tay, Cheng-Gee Koh, Keng-Hwee Chiam
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	zyxin focal adhesion durotaxis rigidity sensing mechanotransduction Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/b822828024704e64a16215e1c227fbfd
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:b822828024704e64a16215e1c227fbfd
record_format	dspace
spelling	oai:doaj.org-article:b822828024704e64a16215e1c227fbfd2021-11-18T09:34:20ZZyxin Is Involved in Fibroblast Rigidity Sensing and Durotaxis2296-634X10.3389/fcell.2021.735298https://doaj.org/article/b822828024704e64a16215e1c227fbfd2021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fcell.2021.735298/fullhttps://doaj.org/toc/2296-634XFocal adhesions (FAs) are specialized structures that enable cells to sense their extracellular matrix rigidity and transmit these signals to the interior of the cells, bringing about actin cytoskeleton reorganization, FA maturation, and cell migration. It is known that cells migrate towards regions of higher substrate rigidity, a phenomenon known as durotaxis. However, the underlying molecular mechanism of durotaxis and how different proteins in the FA are involved remain unclear. Zyxin is a component of the FA that has been implicated in connecting the actin cytoskeleton to the FA. We have found that knocking down zyxin impaired NIH3T3 fibroblast’s ability to sense and respond to changes in extracellular matrix in terms of their FA sizes, cell traction stress magnitudes and F-actin organization. Cell migration speed of zyxin knockdown fibroblasts was also independent of the underlying substrate rigidity, unlike wild type fibroblasts which migrated fastest at an intermediate substrate rigidity of 14 kPa. Wild type fibroblasts exhibited durotaxis by migrating toward regions of increasing substrate rigidity on polyacrylamide gels with substrate rigidity gradient, while zyxin knockdown fibroblasts did not exhibit durotaxis. Therefore, we propose zyxin as an essential protein that is required for rigidity sensing and durotaxis through modulating FA sizes, cell traction stress and F-actin organization.Ai Kia YipSongjing ZhangLor Huai ChongLor Huai ChongElsie CherubaJessie Yong Xing WoonTheng Xuan ChuaCorinna Jie Hui GohHaibo YangChor Yong TayChor Yong TayChor Yong TayChor Yong TayCheng-Gee KohKeng-Hwee ChiamFrontiers Media S.A.articlezyxinfocal adhesiondurotaxisrigidity sensingmechanotransductionBiology (General)QH301-705.5ENFrontiers in Cell and Developmental Biology, Vol 9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	zyxin focal adhesion durotaxis rigidity sensing mechanotransduction Biology (General) QH301-705.5
spellingShingle	zyxin focal adhesion durotaxis rigidity sensing mechanotransduction Biology (General) QH301-705.5 Ai Kia Yip Songjing Zhang Lor Huai Chong Lor Huai Chong Elsie Cheruba Jessie Yong Xing Woon Theng Xuan Chua Corinna Jie Hui Goh Haibo Yang Chor Yong Tay Chor Yong Tay Chor Yong Tay Chor Yong Tay Cheng-Gee Koh Keng-Hwee Chiam Zyxin Is Involved in Fibroblast Rigidity Sensing and Durotaxis
description	Focal adhesions (FAs) are specialized structures that enable cells to sense their extracellular matrix rigidity and transmit these signals to the interior of the cells, bringing about actin cytoskeleton reorganization, FA maturation, and cell migration. It is known that cells migrate towards regions of higher substrate rigidity, a phenomenon known as durotaxis. However, the underlying molecular mechanism of durotaxis and how different proteins in the FA are involved remain unclear. Zyxin is a component of the FA that has been implicated in connecting the actin cytoskeleton to the FA. We have found that knocking down zyxin impaired NIH3T3 fibroblast’s ability to sense and respond to changes in extracellular matrix in terms of their FA sizes, cell traction stress magnitudes and F-actin organization. Cell migration speed of zyxin knockdown fibroblasts was also independent of the underlying substrate rigidity, unlike wild type fibroblasts which migrated fastest at an intermediate substrate rigidity of 14 kPa. Wild type fibroblasts exhibited durotaxis by migrating toward regions of increasing substrate rigidity on polyacrylamide gels with substrate rigidity gradient, while zyxin knockdown fibroblasts did not exhibit durotaxis. Therefore, we propose zyxin as an essential protein that is required for rigidity sensing and durotaxis through modulating FA sizes, cell traction stress and F-actin organization.
format	article
author	Ai Kia Yip Songjing Zhang Lor Huai Chong Lor Huai Chong Elsie Cheruba Jessie Yong Xing Woon Theng Xuan Chua Corinna Jie Hui Goh Haibo Yang Chor Yong Tay Chor Yong Tay Chor Yong Tay Chor Yong Tay Cheng-Gee Koh Keng-Hwee Chiam
author_facet	Ai Kia Yip Songjing Zhang Lor Huai Chong Lor Huai Chong Elsie Cheruba Jessie Yong Xing Woon Theng Xuan Chua Corinna Jie Hui Goh Haibo Yang Chor Yong Tay Chor Yong Tay Chor Yong Tay Chor Yong Tay Cheng-Gee Koh Keng-Hwee Chiam
author_sort	Ai Kia Yip
title	Zyxin Is Involved in Fibroblast Rigidity Sensing and Durotaxis
title_short	Zyxin Is Involved in Fibroblast Rigidity Sensing and Durotaxis
title_full	Zyxin Is Involved in Fibroblast Rigidity Sensing and Durotaxis
title_fullStr	Zyxin Is Involved in Fibroblast Rigidity Sensing and Durotaxis
title_full_unstemmed	Zyxin Is Involved in Fibroblast Rigidity Sensing and Durotaxis
title_sort	zyxin is involved in fibroblast rigidity sensing and durotaxis
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/b822828024704e64a16215e1c227fbfd
work_keys_str_mv	AT aikiayip zyxinisinvolvedinfibroblastrigiditysensinganddurotaxis AT songjingzhang zyxinisinvolvedinfibroblastrigiditysensinganddurotaxis AT lorhuaichong zyxinisinvolvedinfibroblastrigiditysensinganddurotaxis AT lorhuaichong zyxinisinvolvedinfibroblastrigiditysensinganddurotaxis AT elsiecheruba zyxinisinvolvedinfibroblastrigiditysensinganddurotaxis AT jessieyongxingwoon zyxinisinvolvedinfibroblastrigiditysensinganddurotaxis AT thengxuanchua zyxinisinvolvedinfibroblastrigiditysensinganddurotaxis AT corinnajiehuigoh zyxinisinvolvedinfibroblastrigiditysensinganddurotaxis AT haiboyang zyxinisinvolvedinfibroblastrigiditysensinganddurotaxis AT choryongtay zyxinisinvolvedinfibroblastrigiditysensinganddurotaxis AT choryongtay zyxinisinvolvedinfibroblastrigiditysensinganddurotaxis AT choryongtay zyxinisinvolvedinfibroblastrigiditysensinganddurotaxis AT choryongtay zyxinisinvolvedinfibroblastrigiditysensinganddurotaxis AT chenggeekoh zyxinisinvolvedinfibroblastrigiditysensinganddurotaxis AT kenghweechiam zyxinisinvolvedinfibroblastrigiditysensinganddurotaxis
_version_	1718420924098674688

Zyxin Is Involved in Fibroblast Rigidity Sensing and Durotaxis

Ejemplares similares