Demonstrating Principles of Metabolism through Herbicide Treatment of Microalgae

Demonstrating Principles of Metabolism through Herbicide Treatment of Microalgae

An undergraduate laboratory experiment is described which illustrates blockade of a metabolic pathway and the potential environmental impacts of some herbicides. Students use a spectrophotometric assay to examine branched chain amino acid (BCAA) depletion in microalgae cultures treated with sulfosul...

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Autor principal: Betsy Leverett
Formato: article
Lenguaje:EN
Publicado: American Society for Microbiology 2017
Materias:
Special aspects of education
LC8-6691
Biology (General)
QH301-705.5
Acceso en línea:https://doaj.org/article/b828ffeef64841da807eb50f674a158e
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spelling oai:doaj.org-article:b828ffeef64841da807eb50f674a158e2021-11-15T15:04:07ZDemonstrating Principles of Metabolism through Herbicide Treatment of Microalgae10.1128/jmbe.v18i3.13261935-78851935-7877https://doaj.org/article/b828ffeef64841da807eb50f674a158e2017-12-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/jmbe.v18i3.1326https://doaj.org/toc/1935-7877https://doaj.org/toc/1935-7885An undergraduate laboratory experiment is described which illustrates blockade of a metabolic pathway and the potential environmental impacts of some herbicides. Students use a spectrophotometric assay to examine branched chain amino acid (BCAA) depletion in microalgae cultures treated with sulfosulfuron, an herbicide and known inhibitor of BCAA biosynthesis. Students also compare the growth of microalgae with and without sulfosulfuron treatment. The experiment provides student training in basic cell culture, biochemical assay techniques, and the use of a microplate spectrophotometer.Betsy LeverettAmerican Society for MicrobiologyarticleSpecial aspects of educationLC8-6691Biology (General)QH301-705.5ENJournal of Microbiology & Biology Education, Vol 18, Iss 3 (2017)
institution DOAJ
collection DOAJ
language EN
topic Special aspects of education
LC8-6691
Biology (General)
QH301-705.5
spellingShingle Special aspects of education
LC8-6691
Biology (General)
QH301-705.5
Betsy Leverett
Demonstrating Principles of Metabolism through Herbicide Treatment of Microalgae
description An undergraduate laboratory experiment is described which illustrates blockade of a metabolic pathway and the potential environmental impacts of some herbicides. Students use a spectrophotometric assay to examine branched chain amino acid (BCAA) depletion in microalgae cultures treated with sulfosulfuron, an herbicide and known inhibitor of BCAA biosynthesis. Students also compare the growth of microalgae with and without sulfosulfuron treatment. The experiment provides student training in basic cell culture, biochemical assay techniques, and the use of a microplate spectrophotometer.
format article
author Betsy Leverett
author_facet Betsy Leverett
author_sort Betsy Leverett
title Demonstrating Principles of Metabolism through Herbicide Treatment of Microalgae
title_short Demonstrating Principles of Metabolism through Herbicide Treatment of Microalgae
title_full Demonstrating Principles of Metabolism through Herbicide Treatment of Microalgae
title_fullStr Demonstrating Principles of Metabolism through Herbicide Treatment of Microalgae
title_full_unstemmed Demonstrating Principles of Metabolism through Herbicide Treatment of Microalgae
title_sort demonstrating principles of metabolism through herbicide treatment of microalgae
publisher American Society for Microbiology
publishDate 2017
url https://doaj.org/article/b828ffeef64841da807eb50f674a158e
work_keys_str_mv AT betsyleverett demonstratingprinciplesofmetabolismthroughherbicidetreatmentofmicroalgae
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