Infrared-spectroscopic, dynamic near-field microscopy of living cells and nanoparticles in water

Abstract Infrared fingerprint spectra can reveal the chemical nature of materials down to 20-nm detail, far below the diffraction limit, when probed by scattering-type scanning near-field optical microscopy (s-SNOM). But this was impossible with living cells or aqueous processes as in corrosion, due...

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Autores principales:	Korbinian J. Kaltenecker, Thorsten Gölz, Enrico Bau, Fritz Keilmann
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b8303eac027c4aaba15f72b7d6e69052
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spelling	oai:doaj.org-article:b8303eac027c4aaba15f72b7d6e690522021-11-14T12:18:03ZInfrared-spectroscopic, dynamic near-field microscopy of living cells and nanoparticles in water10.1038/s41598-021-01425-w2045-2322https://doaj.org/article/b8303eac027c4aaba15f72b7d6e690522021-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-01425-whttps://doaj.org/toc/2045-2322Abstract Infrared fingerprint spectra can reveal the chemical nature of materials down to 20-nm detail, far below the diffraction limit, when probed by scattering-type scanning near-field optical microscopy (s-SNOM). But this was impossible with living cells or aqueous processes as in corrosion, due to water-related absorption and tip contamination. Here, we demonstrate infrared s-SNOM of water-suspended objects by probing them through a 10-nm thick SiN membrane. This separator stretches freely over up to 250 µm, providing an upper, stable surface to the scanning tip, while its lower surface is in contact with the liquid and localises adhering objects. We present its proof-of-principle applicability in biology by observing simply drop-casted, living E. coli in nutrient medium, as well as living A549 cancer cells, as they divide, move and develop rich sub-cellular morphology and adhesion patterns, at 150 nm resolution. Their infrared spectra reveal the local abundances of water, proteins, and lipids within a depth of ca. 100 nm below the SiN membrane, as we verify by analysing well-defined, suspended polymer spheres and through model calculations. SiN-membrane based s-SNOM thus establishes a novel tool of live cell nano-imaging that returns structure, dynamics and chemical composition. This method should benefit the nanoscale analysis of any aqueous system, from physics to medicine.Korbinian J. KalteneckerThorsten GölzEnrico BauFritz KeilmannNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Korbinian J. Kaltenecker Thorsten Gölz Enrico Bau Fritz Keilmann Infrared-spectroscopic, dynamic near-field microscopy of living cells and nanoparticles in water
description	Abstract Infrared fingerprint spectra can reveal the chemical nature of materials down to 20-nm detail, far below the diffraction limit, when probed by scattering-type scanning near-field optical microscopy (s-SNOM). But this was impossible with living cells or aqueous processes as in corrosion, due to water-related absorption and tip contamination. Here, we demonstrate infrared s-SNOM of water-suspended objects by probing them through a 10-nm thick SiN membrane. This separator stretches freely over up to 250 µm, providing an upper, stable surface to the scanning tip, while its lower surface is in contact with the liquid and localises adhering objects. We present its proof-of-principle applicability in biology by observing simply drop-casted, living E. coli in nutrient medium, as well as living A549 cancer cells, as they divide, move and develop rich sub-cellular morphology and adhesion patterns, at 150 nm resolution. Their infrared spectra reveal the local abundances of water, proteins, and lipids within a depth of ca. 100 nm below the SiN membrane, as we verify by analysing well-defined, suspended polymer spheres and through model calculations. SiN-membrane based s-SNOM thus establishes a novel tool of live cell nano-imaging that returns structure, dynamics and chemical composition. This method should benefit the nanoscale analysis of any aqueous system, from physics to medicine.
format	article
author	Korbinian J. Kaltenecker Thorsten Gölz Enrico Bau Fritz Keilmann
author_facet	Korbinian J. Kaltenecker Thorsten Gölz Enrico Bau Fritz Keilmann
author_sort	Korbinian J. Kaltenecker
title	Infrared-spectroscopic, dynamic near-field microscopy of living cells and nanoparticles in water
title_short	Infrared-spectroscopic, dynamic near-field microscopy of living cells and nanoparticles in water
title_full	Infrared-spectroscopic, dynamic near-field microscopy of living cells and nanoparticles in water
title_fullStr	Infrared-spectroscopic, dynamic near-field microscopy of living cells and nanoparticles in water
title_full_unstemmed	Infrared-spectroscopic, dynamic near-field microscopy of living cells and nanoparticles in water
title_sort	infrared-spectroscopic, dynamic near-field microscopy of living cells and nanoparticles in water
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/b8303eac027c4aaba15f72b7d6e69052
work_keys_str_mv	AT korbinianjkaltenecker infraredspectroscopicdynamicnearfieldmicroscopyoflivingcellsandnanoparticlesinwater AT thorstengolz infraredspectroscopicdynamicnearfieldmicroscopyoflivingcellsandnanoparticlesinwater AT enricobau infraredspectroscopicdynamicnearfieldmicroscopyoflivingcellsandnanoparticlesinwater AT fritzkeilmann infraredspectroscopicdynamicnearfieldmicroscopyoflivingcellsandnanoparticlesinwater
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Infrared-spectroscopic, dynamic near-field microscopy of living cells and nanoparticles in water

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