Improvement of an Effective Protocol for Directed Differentiation of Human Adipose Tissue-Derived Adult Mesenchymal Stem Cells to Corneal Endothelial Cells

Corneal disease affects 12.5 million individuals worldwide, with 2 million new cases each year. The standard treatment consists of a corneal transplantation from a human donor; however, the worldwide demand significantly exceeds the available supply. Lamellar endothelial keratoplasty, the replacemen...

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Autores principales:	Cadenas-Martin Marta, Moratilla Adrian, Fernández-Delgado Jorge, Arnalich-Montiel Francisco, Maria P. De Miguel
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	cell reprogramming differentiation adipose tissue-derived mesenchymal stem cells cornea corneal endothelium Biology (General) QH301-705.5 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/b8355fc5836744939aeae18d7fea7165
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spelling	oai:doaj.org-article:b8355fc5836744939aeae18d7fea71652021-11-11T17:24:06ZImprovement of an Effective Protocol for Directed Differentiation of Human Adipose Tissue-Derived Adult Mesenchymal Stem Cells to Corneal Endothelial Cells10.3390/ijms2221119821422-00671661-6596https://doaj.org/article/b8355fc5836744939aeae18d7fea71652021-11-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/21/11982https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067Corneal disease affects 12.5 million individuals worldwide, with 2 million new cases each year. The standard treatment consists of a corneal transplantation from a human donor; however, the worldwide demand significantly exceeds the available supply. Lamellar endothelial keratoplasty, the replacement of only the endothelial layer of the cornea, can partially solve the problem. Progressive efforts have succeeded in expanding hCECs; however, the ability to expand hCECs is still limited, and new sources of CECs are being sought. Crucial advances have been achieved by the directed differentiation of embryonic or induced pluripotent stem cells, but these cells have disadvantages, such as the use of oncogenes, and are still difficult to establish. We aimed to transfer such knowledge to obtain hCECs from adipose tissue-derived adult mesenchymal stem cells (ADSC) by modifying four previously published procedures. We present several protocols capable of the directed differentiation of human ADSCs to hCECs. In our hands, the protocol by Ali et al. was the best adapted to such differentiation in terms of efficiency, time, and financial cost; however, the protocol by Wagoner et al. was the best for CEC marker expression. Our results broaden the type of cells of autologous extraocular origin that could be employed in the clinical setting for corneal endothelial deficiency.Cadenas-Martin MartaMoratilla AdrianFernández-Delgado JorgeArnalich-Montiel FranciscoMaria P. De MiguelMDPI AGarticlecell reprogrammingdifferentiationadipose tissue-derived mesenchymal stem cellscorneacorneal endotheliumBiology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 11982, p 11982 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	cell reprogramming differentiation adipose tissue-derived mesenchymal stem cells cornea corneal endothelium Biology (General) QH301-705.5 Chemistry QD1-999
spellingShingle	cell reprogramming differentiation adipose tissue-derived mesenchymal stem cells cornea corneal endothelium Biology (General) QH301-705.5 Chemistry QD1-999 Cadenas-Martin Marta Moratilla Adrian Fernández-Delgado Jorge Arnalich-Montiel Francisco Maria P. De Miguel Improvement of an Effective Protocol for Directed Differentiation of Human Adipose Tissue-Derived Adult Mesenchymal Stem Cells to Corneal Endothelial Cells
description	Corneal disease affects 12.5 million individuals worldwide, with 2 million new cases each year. The standard treatment consists of a corneal transplantation from a human donor; however, the worldwide demand significantly exceeds the available supply. Lamellar endothelial keratoplasty, the replacement of only the endothelial layer of the cornea, can partially solve the problem. Progressive efforts have succeeded in expanding hCECs; however, the ability to expand hCECs is still limited, and new sources of CECs are being sought. Crucial advances have been achieved by the directed differentiation of embryonic or induced pluripotent stem cells, but these cells have disadvantages, such as the use of oncogenes, and are still difficult to establish. We aimed to transfer such knowledge to obtain hCECs from adipose tissue-derived adult mesenchymal stem cells (ADSC) by modifying four previously published procedures. We present several protocols capable of the directed differentiation of human ADSCs to hCECs. In our hands, the protocol by Ali et al. was the best adapted to such differentiation in terms of efficiency, time, and financial cost; however, the protocol by Wagoner et al. was the best for CEC marker expression. Our results broaden the type of cells of autologous extraocular origin that could be employed in the clinical setting for corneal endothelial deficiency.
format	article
author	Cadenas-Martin Marta Moratilla Adrian Fernández-Delgado Jorge Arnalich-Montiel Francisco Maria P. De Miguel
author_facet	Cadenas-Martin Marta Moratilla Adrian Fernández-Delgado Jorge Arnalich-Montiel Francisco Maria P. De Miguel
author_sort	Cadenas-Martin Marta
title	Improvement of an Effective Protocol for Directed Differentiation of Human Adipose Tissue-Derived Adult Mesenchymal Stem Cells to Corneal Endothelial Cells
title_short	Improvement of an Effective Protocol for Directed Differentiation of Human Adipose Tissue-Derived Adult Mesenchymal Stem Cells to Corneal Endothelial Cells
title_full	Improvement of an Effective Protocol for Directed Differentiation of Human Adipose Tissue-Derived Adult Mesenchymal Stem Cells to Corneal Endothelial Cells
title_fullStr	Improvement of an Effective Protocol for Directed Differentiation of Human Adipose Tissue-Derived Adult Mesenchymal Stem Cells to Corneal Endothelial Cells
title_full_unstemmed	Improvement of an Effective Protocol for Directed Differentiation of Human Adipose Tissue-Derived Adult Mesenchymal Stem Cells to Corneal Endothelial Cells
title_sort	improvement of an effective protocol for directed differentiation of human adipose tissue-derived adult mesenchymal stem cells to corneal endothelial cells
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/b8355fc5836744939aeae18d7fea7165
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Improvement of an Effective Protocol for Directed Differentiation of Human Adipose Tissue-Derived Adult Mesenchymal Stem Cells to Corneal Endothelial Cells

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