Psoralen induced cell cycle arrest by modulating Wnt/β-catenin pathway in breast cancer cells
Abstract Psoralen could inhibit the proliferation of human breast cancer cells, however, the molecular mechanism was unclear. We evaluated the anti-proliferative effects of psoralen by MTT, plate colony formation assay and cell cycle analysis in MCF-7 and MDA-MB-231 cells. The effects of psoralen on...
Guardado en:
| Autores principales: | , , , , , , , , , , |
|---|---|
| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
Nature Portfolio
2018
|
| Materias: | |
| Acceso en línea: | https://doaj.org/article/b8361f6867eb4f2986ea1f3a4c758202 |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| id |
oai:doaj.org-article:b8361f6867eb4f2986ea1f3a4c758202 |
|---|---|
| record_format |
dspace |
| spelling |
oai:doaj.org-article:b8361f6867eb4f2986ea1f3a4c7582022021-12-02T15:09:12ZPsoralen induced cell cycle arrest by modulating Wnt/β-catenin pathway in breast cancer cells10.1038/s41598-018-32438-72045-2322https://doaj.org/article/b8361f6867eb4f2986ea1f3a4c7582022018-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-32438-7https://doaj.org/toc/2045-2322Abstract Psoralen could inhibit the proliferation of human breast cancer cells, however, the molecular mechanism was unclear. We evaluated the anti-proliferative effects of psoralen by MTT, plate colony formation assay and cell cycle analysis in MCF-7 and MDA-MB-231 cells. The effects of psoralen on activation of Wnt/β-catenin and the related target genes were examined by quantitative real-time PCR, western blotting and cell immunofluorescence. The tumor growth was conducted in BALB/c nude mice and the pathological changes of heart, liver and kidney were also observed. Our results demonstrate that psoralen significantly inhibited cell proliferation by inducing G0/G1 phase arrest in MCF-7 cells and G2/M phase arrest in MDA-MB-231 cells. The expression of Fra-1 was reduced and Axin2 was promoted both in MCF-7 and MDA-MB-231 cells after psoralen treatment. The cytoplasmic accumulation and nuclear translocation of β-catenin were significantly reduced by psoralen. Psoralen increased the levels of phospho-(Y142) β-catenin, while decreased the expression of total β-catenin and its downstream target Fra-1 in vitro and vivo. Moreover, psoralen didn’t cause any significant toxicity at the effective concentration. Overall, our results might provide theoretical basis for clinical application of psoralen in breast cancer.Xiaohong WangChengfeng XuYitong HuaKai ChengYingzhe ZhangJian LiuYong HanSong LiuGuoqiang ZhangShujian XuZhenlin YangNature PortfolioarticleBreast CancerCell CyclePsoralen TreatmentPlate Colony Formation AssayRelative Target GeneMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-7 (2018) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
Breast Cancer Cell Cycle Psoralen Treatment Plate Colony Formation Assay Relative Target Gene Medicine R Science Q |
| spellingShingle |
Breast Cancer Cell Cycle Psoralen Treatment Plate Colony Formation Assay Relative Target Gene Medicine R Science Q Xiaohong Wang Chengfeng Xu Yitong Hua Kai Cheng Yingzhe Zhang Jian Liu Yong Han Song Liu Guoqiang Zhang Shujian Xu Zhenlin Yang Psoralen induced cell cycle arrest by modulating Wnt/β-catenin pathway in breast cancer cells |
| description |
Abstract Psoralen could inhibit the proliferation of human breast cancer cells, however, the molecular mechanism was unclear. We evaluated the anti-proliferative effects of psoralen by MTT, plate colony formation assay and cell cycle analysis in MCF-7 and MDA-MB-231 cells. The effects of psoralen on activation of Wnt/β-catenin and the related target genes were examined by quantitative real-time PCR, western blotting and cell immunofluorescence. The tumor growth was conducted in BALB/c nude mice and the pathological changes of heart, liver and kidney were also observed. Our results demonstrate that psoralen significantly inhibited cell proliferation by inducing G0/G1 phase arrest in MCF-7 cells and G2/M phase arrest in MDA-MB-231 cells. The expression of Fra-1 was reduced and Axin2 was promoted both in MCF-7 and MDA-MB-231 cells after psoralen treatment. The cytoplasmic accumulation and nuclear translocation of β-catenin were significantly reduced by psoralen. Psoralen increased the levels of phospho-(Y142) β-catenin, while decreased the expression of total β-catenin and its downstream target Fra-1 in vitro and vivo. Moreover, psoralen didn’t cause any significant toxicity at the effective concentration. Overall, our results might provide theoretical basis for clinical application of psoralen in breast cancer. |
| format |
article |
| author |
Xiaohong Wang Chengfeng Xu Yitong Hua Kai Cheng Yingzhe Zhang Jian Liu Yong Han Song Liu Guoqiang Zhang Shujian Xu Zhenlin Yang |
| author_facet |
Xiaohong Wang Chengfeng Xu Yitong Hua Kai Cheng Yingzhe Zhang Jian Liu Yong Han Song Liu Guoqiang Zhang Shujian Xu Zhenlin Yang |
| author_sort |
Xiaohong Wang |
| title |
Psoralen induced cell cycle arrest by modulating Wnt/β-catenin pathway in breast cancer cells |
| title_short |
Psoralen induced cell cycle arrest by modulating Wnt/β-catenin pathway in breast cancer cells |
| title_full |
Psoralen induced cell cycle arrest by modulating Wnt/β-catenin pathway in breast cancer cells |
| title_fullStr |
Psoralen induced cell cycle arrest by modulating Wnt/β-catenin pathway in breast cancer cells |
| title_full_unstemmed |
Psoralen induced cell cycle arrest by modulating Wnt/β-catenin pathway in breast cancer cells |
| title_sort |
psoralen induced cell cycle arrest by modulating wnt/β-catenin pathway in breast cancer cells |
| publisher |
Nature Portfolio |
| publishDate |
2018 |
| url |
https://doaj.org/article/b8361f6867eb4f2986ea1f3a4c758202 |
| work_keys_str_mv |
AT xiaohongwang psoraleninducedcellcyclearrestbymodulatingwntbcateninpathwayinbreastcancercells AT chengfengxu psoraleninducedcellcyclearrestbymodulatingwntbcateninpathwayinbreastcancercells AT yitonghua psoraleninducedcellcyclearrestbymodulatingwntbcateninpathwayinbreastcancercells AT kaicheng psoraleninducedcellcyclearrestbymodulatingwntbcateninpathwayinbreastcancercells AT yingzhezhang psoraleninducedcellcyclearrestbymodulatingwntbcateninpathwayinbreastcancercells AT jianliu psoraleninducedcellcyclearrestbymodulatingwntbcateninpathwayinbreastcancercells AT yonghan psoraleninducedcellcyclearrestbymodulatingwntbcateninpathwayinbreastcancercells AT songliu psoraleninducedcellcyclearrestbymodulatingwntbcateninpathwayinbreastcancercells AT guoqiangzhang psoraleninducedcellcyclearrestbymodulatingwntbcateninpathwayinbreastcancercells AT shujianxu psoraleninducedcellcyclearrestbymodulatingwntbcateninpathwayinbreastcancercells AT zhenlinyang psoraleninducedcellcyclearrestbymodulatingwntbcateninpathwayinbreastcancercells |
| _version_ |
1718387897564921856 |