Antimicrobial responses of peripheral and central nervous system glia against Staphylococcus aureus

Abstract Staphylococcus aureus infections of the central nervous system are serious and can be fatal. S. aureus is commonly present in the nasal cavity, and after injury to the nasal epithelium it can rapidly invade the brain via the olfactory nerve. The trigeminal nerve constitutes another potentia...

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Autores principales:	Indra N. Choudhury, Anu Chacko, Ali Delbaz, Mo Chen, Souptik Basu, James A. St John, Flavia Huygens, Jenny A. K. Ekberg
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b83b820b19fc4859b88354c0b72d28da
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spelling	oai:doaj.org-article:b83b820b19fc4859b88354c0b72d28da2021-12-02T14:59:09ZAntimicrobial responses of peripheral and central nervous system glia against Staphylococcus aureus10.1038/s41598-021-90252-02045-2322https://doaj.org/article/b83b820b19fc4859b88354c0b72d28da2021-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-90252-0https://doaj.org/toc/2045-2322Abstract Staphylococcus aureus infections of the central nervous system are serious and can be fatal. S. aureus is commonly present in the nasal cavity, and after injury to the nasal epithelium it can rapidly invade the brain via the olfactory nerve. The trigeminal nerve constitutes another potential route of brain infection. The glia of these nerves, olfactory ensheathing cells (OECs) and trigeminal nerve Schwann cells (TgSCs), as well as astrocytes populating the glia limitans layer, can phagocytose bacteria. Whilst some glial responses to S. aureus have been studied, the specific responses of different glial types are unknown. Here, we compared how primary mouse OECs, TgSCs, astrocytes and microglia responded to S. aureus. All glial types internalized the bacteria within phagolysosomes, and S. aureus-conjugated BioParticles could be tracked with subtle but significant differences in time-course of phagocytosis between glial types. Live bacteria could be isolated from all glia after 24 h in culture, and microglia, OECs and TgSCs exhibited better protection against intracellular S. aureus survival than astrocytes. All glial types responded to the bacteria by cytokine secretion. Overall, OECs secreted the lowest level of cytokines, suggesting that these cells, despite showing strong capacity for phagocytosis, have immunomodulatory functions that can be relevant for neural repair.Indra N. ChoudhuryAnu ChackoAli DelbazMo ChenSouptik BasuJames A. St JohnFlavia HuygensJenny A. K. EkbergNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-15 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Indra N. Choudhury Anu Chacko Ali Delbaz Mo Chen Souptik Basu James A. St John Flavia Huygens Jenny A. K. Ekberg Antimicrobial responses of peripheral and central nervous system glia against Staphylococcus aureus
description	Abstract Staphylococcus aureus infections of the central nervous system are serious and can be fatal. S. aureus is commonly present in the nasal cavity, and after injury to the nasal epithelium it can rapidly invade the brain via the olfactory nerve. The trigeminal nerve constitutes another potential route of brain infection. The glia of these nerves, olfactory ensheathing cells (OECs) and trigeminal nerve Schwann cells (TgSCs), as well as astrocytes populating the glia limitans layer, can phagocytose bacteria. Whilst some glial responses to S. aureus have been studied, the specific responses of different glial types are unknown. Here, we compared how primary mouse OECs, TgSCs, astrocytes and microglia responded to S. aureus. All glial types internalized the bacteria within phagolysosomes, and S. aureus-conjugated BioParticles could be tracked with subtle but significant differences in time-course of phagocytosis between glial types. Live bacteria could be isolated from all glia after 24 h in culture, and microglia, OECs and TgSCs exhibited better protection against intracellular S. aureus survival than astrocytes. All glial types responded to the bacteria by cytokine secretion. Overall, OECs secreted the lowest level of cytokines, suggesting that these cells, despite showing strong capacity for phagocytosis, have immunomodulatory functions that can be relevant for neural repair.
format	article
author	Indra N. Choudhury Anu Chacko Ali Delbaz Mo Chen Souptik Basu James A. St John Flavia Huygens Jenny A. K. Ekberg
author_facet	Indra N. Choudhury Anu Chacko Ali Delbaz Mo Chen Souptik Basu James A. St John Flavia Huygens Jenny A. K. Ekberg
author_sort	Indra N. Choudhury
title	Antimicrobial responses of peripheral and central nervous system glia against Staphylococcus aureus
title_short	Antimicrobial responses of peripheral and central nervous system glia against Staphylococcus aureus
title_full	Antimicrobial responses of peripheral and central nervous system glia against Staphylococcus aureus
title_fullStr	Antimicrobial responses of peripheral and central nervous system glia against Staphylococcus aureus
title_full_unstemmed	Antimicrobial responses of peripheral and central nervous system glia against Staphylococcus aureus
title_sort	antimicrobial responses of peripheral and central nervous system glia against staphylococcus aureus
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/b83b820b19fc4859b88354c0b72d28da
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Antimicrobial responses of peripheral and central nervous system glia against Staphylococcus aureus

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