The C/EBPβ LIP isoform rescues loss of C/EBPβ function in the mouse

Abstract The transcription factor C/EBPβ regulates hematopoiesis, bone, liver, fat, and skin homeostasis, and female reproduction. C/EBPβ protein expression from its single transcript occurs by alternative in-frame translation initiation at consecutive start sites to generate three isoforms, two lon...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Valérie Bégay, Christian Baumeier, Karin Zimmermann, Arnd Heuser, Achim Leutz
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b849d73d25b04df9b8dfc73c878720df
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:b849d73d25b04df9b8dfc73c878720df
record_format	dspace
spelling	oai:doaj.org-article:b849d73d25b04df9b8dfc73c878720df2021-12-02T15:08:38ZThe C/EBPβ LIP isoform rescues loss of C/EBPβ function in the mouse10.1038/s41598-018-26579-y2045-2322https://doaj.org/article/b849d73d25b04df9b8dfc73c878720df2018-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-26579-yhttps://doaj.org/toc/2045-2322Abstract The transcription factor C/EBPβ regulates hematopoiesis, bone, liver, fat, and skin homeostasis, and female reproduction. C/EBPβ protein expression from its single transcript occurs by alternative in-frame translation initiation at consecutive start sites to generate three isoforms, two long (LAP*, LAP) and one truncated (LIP), with the same C-terminal bZip dimerization domain. The long C/EBPβ isoforms are considered gene activators, whereas the LIP isoform reportedly acts as a dominant-negative repressor. Here, we tested the putative repressor functions of the C/EBPβ LIP isoform in mice by comparing monoallelic WT or LIP knockin mice with Cebpb knockout mice, in combination with monoallelic Cebpa mice. The C/EBPβ LIP isoform was sufficient to function in coordination with C/EBPα in murine development, adipose tissue and sebocyte differentiation, and female fertility. Thus, the C/EBPβ LIP isoform likely has more physiological functions than its currently known role as a dominant-negative inhibitor, which are more complex than anticipated.Valérie BégayChristian BaumeierKarin ZimmermannArnd HeuserAchim LeutzNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-10 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Valérie Bégay Christian Baumeier Karin Zimmermann Arnd Heuser Achim Leutz The C/EBPβ LIP isoform rescues loss of C/EBPβ function in the mouse
description	Abstract The transcription factor C/EBPβ regulates hematopoiesis, bone, liver, fat, and skin homeostasis, and female reproduction. C/EBPβ protein expression from its single transcript occurs by alternative in-frame translation initiation at consecutive start sites to generate three isoforms, two long (LAP*, LAP) and one truncated (LIP), with the same C-terminal bZip dimerization domain. The long C/EBPβ isoforms are considered gene activators, whereas the LIP isoform reportedly acts as a dominant-negative repressor. Here, we tested the putative repressor functions of the C/EBPβ LIP isoform in mice by comparing monoallelic WT or LIP knockin mice with Cebpb knockout mice, in combination with monoallelic Cebpa mice. The C/EBPβ LIP isoform was sufficient to function in coordination with C/EBPα in murine development, adipose tissue and sebocyte differentiation, and female fertility. Thus, the C/EBPβ LIP isoform likely has more physiological functions than its currently known role as a dominant-negative inhibitor, which are more complex than anticipated.
format	article
author	Valérie Bégay Christian Baumeier Karin Zimmermann Arnd Heuser Achim Leutz
author_facet	Valérie Bégay Christian Baumeier Karin Zimmermann Arnd Heuser Achim Leutz
author_sort	Valérie Bégay
title	The C/EBPβ LIP isoform rescues loss of C/EBPβ function in the mouse
title_short	The C/EBPβ LIP isoform rescues loss of C/EBPβ function in the mouse
title_full	The C/EBPβ LIP isoform rescues loss of C/EBPβ function in the mouse
title_fullStr	The C/EBPβ LIP isoform rescues loss of C/EBPβ function in the mouse
title_full_unstemmed	The C/EBPβ LIP isoform rescues loss of C/EBPβ function in the mouse
title_sort	c/ebpβ lip isoform rescues loss of c/ebpβ function in the mouse
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/b849d73d25b04df9b8dfc73c878720df
work_keys_str_mv	AT valeriebegay thecebpblipisoformrescueslossofcebpbfunctioninthemouse AT christianbaumeier thecebpblipisoformrescueslossofcebpbfunctioninthemouse AT karinzimmermann thecebpblipisoformrescueslossofcebpbfunctioninthemouse AT arndheuser thecebpblipisoformrescueslossofcebpbfunctioninthemouse AT achimleutz thecebpblipisoformrescueslossofcebpbfunctioninthemouse AT valeriebegay cebpblipisoformrescueslossofcebpbfunctioninthemouse AT christianbaumeier cebpblipisoformrescueslossofcebpbfunctioninthemouse AT karinzimmermann cebpblipisoformrescueslossofcebpbfunctioninthemouse AT arndheuser cebpblipisoformrescueslossofcebpbfunctioninthemouse AT achimleutz cebpblipisoformrescueslossofcebpbfunctioninthemouse
_version_	1718388083209011200

The C/EBPβ LIP isoform rescues loss of C/EBPβ function in the mouse

Ejemplares similares