Functional mapping of protein kinase A reveals its importance in adult Schistosoma mansoni motor activity.

Cyclic AMP (cAMP)-dependent protein kinase/protein kinase A (PKA) is the major transducer of cAMP signalling in eukaryotic cells. Here, using laser scanning confocal microscopy and 'smart' anti-phospho PKA antibodies that exclusively detect activated PKA, we provide a detailed in situ anal...

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Autores principales:	Paulu S R de Saram, Margarida Ressurreição, Angela J Davies, David Rollinson, Aidan M Emery, Anthony J Walker
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270
Acceso en línea:	https://doaj.org/article/b84af1e1bece4e5fb837f2da0b93fff0
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spelling	oai:doaj.org-article:b84af1e1bece4e5fb837f2da0b93fff02021-11-18T09:15:29ZFunctional mapping of protein kinase A reveals its importance in adult Schistosoma mansoni motor activity.1935-27271935-273510.1371/journal.pntd.0001988https://doaj.org/article/b84af1e1bece4e5fb837f2da0b93fff02013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23326613/?tool=EBIhttps://doaj.org/toc/1935-2727https://doaj.org/toc/1935-2735Cyclic AMP (cAMP)-dependent protein kinase/protein kinase A (PKA) is the major transducer of cAMP signalling in eukaryotic cells. Here, using laser scanning confocal microscopy and 'smart' anti-phospho PKA antibodies that exclusively detect activated PKA, we provide a detailed in situ analysis of PKA signalling in intact adult Schistosoma mansoni, a causative agent of debilitating human intestinal schistosomiasis. In both adult male and female worms, activated PKA was consistently found associated with the tegument, oral and ventral suckers, oesophagus and somatic musculature. In addition, the seminal vesicle and gynaecophoric canal muscles of the male displayed activated PKA whereas in female worms activated PKA localized to the ootype wall, the ovary, and the uterus particularly around eggs during expulsion. Exposure of live worms to the PKA activator forskolin (50 µM) resulted in striking PKA activation in the central and peripheral nervous system including at nerve endings at/near the tegument surface. Such neuronal PKA activation was also observed without forskolin treatment, but only in a single batch of worms. In addition, PKA activation within the central and peripheral nervous systems visibly increased within 15 min of worm-pair separation when compared to that observed in closely coupled worm pairs. Finally, exposure of adult worms to forskolin induced hyperkinesias in a time and dose dependent manner with 100 µM forskolin significantly increasing the frequency of gross worm movements to 5.3 times that of control worms (P≤0.001). Collectively these data are consistent with PKA playing a central part in motor activity and neuronal communication, and possibly interplay between these two systems in S. mansoni. This study, the first to localize a protein kinase when exclusively in an activated state in adult S. mansoni, provides valuable insight into the intricacies of functional protein kinase signalling in the context of whole schistosome physiology.Paulu S R de SaramMargarida RessurreiçãoAngela J DaviesDavid RollinsonAidan M EmeryAnthony J WalkerPublic Library of Science (PLoS)articleArctic medicine. Tropical medicineRC955-962Public aspects of medicineRA1-1270ENPLoS Neglected Tropical Diseases, Vol 7, Iss 1, p e1988 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270
spellingShingle	Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Paulu S R de Saram Margarida Ressurreição Angela J Davies David Rollinson Aidan M Emery Anthony J Walker Functional mapping of protein kinase A reveals its importance in adult Schistosoma mansoni motor activity.
description	Cyclic AMP (cAMP)-dependent protein kinase/protein kinase A (PKA) is the major transducer of cAMP signalling in eukaryotic cells. Here, using laser scanning confocal microscopy and 'smart' anti-phospho PKA antibodies that exclusively detect activated PKA, we provide a detailed in situ analysis of PKA signalling in intact adult Schistosoma mansoni, a causative agent of debilitating human intestinal schistosomiasis. In both adult male and female worms, activated PKA was consistently found associated with the tegument, oral and ventral suckers, oesophagus and somatic musculature. In addition, the seminal vesicle and gynaecophoric canal muscles of the male displayed activated PKA whereas in female worms activated PKA localized to the ootype wall, the ovary, and the uterus particularly around eggs during expulsion. Exposure of live worms to the PKA activator forskolin (50 µM) resulted in striking PKA activation in the central and peripheral nervous system including at nerve endings at/near the tegument surface. Such neuronal PKA activation was also observed without forskolin treatment, but only in a single batch of worms. In addition, PKA activation within the central and peripheral nervous systems visibly increased within 15 min of worm-pair separation when compared to that observed in closely coupled worm pairs. Finally, exposure of adult worms to forskolin induced hyperkinesias in a time and dose dependent manner with 100 µM forskolin significantly increasing the frequency of gross worm movements to 5.3 times that of control worms (P≤0.001). Collectively these data are consistent with PKA playing a central part in motor activity and neuronal communication, and possibly interplay between these two systems in S. mansoni. This study, the first to localize a protein kinase when exclusively in an activated state in adult S. mansoni, provides valuable insight into the intricacies of functional protein kinase signalling in the context of whole schistosome physiology.
format	article
author	Paulu S R de Saram Margarida Ressurreição Angela J Davies David Rollinson Aidan M Emery Anthony J Walker
author_facet	Paulu S R de Saram Margarida Ressurreição Angela J Davies David Rollinson Aidan M Emery Anthony J Walker
author_sort	Paulu S R de Saram
title	Functional mapping of protein kinase A reveals its importance in adult Schistosoma mansoni motor activity.
title_short	Functional mapping of protein kinase A reveals its importance in adult Schistosoma mansoni motor activity.
title_full	Functional mapping of protein kinase A reveals its importance in adult Schistosoma mansoni motor activity.
title_fullStr	Functional mapping of protein kinase A reveals its importance in adult Schistosoma mansoni motor activity.
title_full_unstemmed	Functional mapping of protein kinase A reveals its importance in adult Schistosoma mansoni motor activity.
title_sort	functional mapping of protein kinase a reveals its importance in adult schistosoma mansoni motor activity.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/b84af1e1bece4e5fb837f2da0b93fff0
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Functional mapping of protein kinase A reveals its importance in adult Schistosoma mansoni motor activity.

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