Phosphorescent Ir(III) complexes conjugated with oligoarginine peptides serve as optical probes for in vivo microvascular imaging

Abstract Imaging the vascular structures of organ and tumor tissues is extremely important for assessing various pathological conditions. Herein we present the new vascular imaging probe BTQ-Rn (n = 8, 12, 16), a phosphorescent Ir(III) complex containing an oligoarginine peptide as a ligand. This mi...

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Autores principales:	Mami Yasukagawa, Aya Shimada, Shuichi Shiozaki, Seiji Tobita, Toshitada Yoshihara
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b84bc34270044a4284275b59be771f9d
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spelling	oai:doaj.org-article:b84bc34270044a4284275b59be771f9d2021-12-02T13:20:03ZPhosphorescent Ir(III) complexes conjugated with oligoarginine peptides serve as optical probes for in vivo microvascular imaging10.1038/s41598-021-84115-x2045-2322https://doaj.org/article/b84bc34270044a4284275b59be771f9d2021-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-84115-xhttps://doaj.org/toc/2045-2322Abstract Imaging the vascular structures of organ and tumor tissues is extremely important for assessing various pathological conditions. Herein we present the new vascular imaging probe BTQ-Rn (n = 8, 12, 16), a phosphorescent Ir(III) complex containing an oligoarginine peptide as a ligand. This microvasculature staining probe can be chemically synthesized, unlike the commonly used tomato lectins labeled with a fluorophore such as fluorescein isothiocyanate (FITC). Intravenous administration of BTQ-R12 to mice and subsequent confocal luminescence microscope measurements enabled in vivo vascular imaging of tumors and various organs, including kidney, liver and pancreas. Dual color imaging of hepatic tissues of living mice fed a high-fat diet using BTQ-R12 and the lipid droplet-specific probe PC6S revealed small and large lipid droplets in the hepatocytes, causing distortion of the sinusoidal structure. BTQ-R12 selectively stains vascular endothelium and thus allows longer-term vascular network imaging compared to fluorescent dextran with a molecular weight of 70 kDa that circulate in the bloodstream. Furthermore, time-gated measurements using this phosphorescent vascular probe enabled imaging of blood vessel structures without interference from autofluorescence.Mami YasukagawaAya ShimadaShuichi ShiozakiSeiji TobitaToshitada YoshiharaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Mami Yasukagawa Aya Shimada Shuichi Shiozaki Seiji Tobita Toshitada Yoshihara Phosphorescent Ir(III) complexes conjugated with oligoarginine peptides serve as optical probes for in vivo microvascular imaging
description	Abstract Imaging the vascular structures of organ and tumor tissues is extremely important for assessing various pathological conditions. Herein we present the new vascular imaging probe BTQ-Rn (n = 8, 12, 16), a phosphorescent Ir(III) complex containing an oligoarginine peptide as a ligand. This microvasculature staining probe can be chemically synthesized, unlike the commonly used tomato lectins labeled with a fluorophore such as fluorescein isothiocyanate (FITC). Intravenous administration of BTQ-R12 to mice and subsequent confocal luminescence microscope measurements enabled in vivo vascular imaging of tumors and various organs, including kidney, liver and pancreas. Dual color imaging of hepatic tissues of living mice fed a high-fat diet using BTQ-R12 and the lipid droplet-specific probe PC6S revealed small and large lipid droplets in the hepatocytes, causing distortion of the sinusoidal structure. BTQ-R12 selectively stains vascular endothelium and thus allows longer-term vascular network imaging compared to fluorescent dextran with a molecular weight of 70 kDa that circulate in the bloodstream. Furthermore, time-gated measurements using this phosphorescent vascular probe enabled imaging of blood vessel structures without interference from autofluorescence.
format	article
author	Mami Yasukagawa Aya Shimada Shuichi Shiozaki Seiji Tobita Toshitada Yoshihara
author_facet	Mami Yasukagawa Aya Shimada Shuichi Shiozaki Seiji Tobita Toshitada Yoshihara
author_sort	Mami Yasukagawa
title	Phosphorescent Ir(III) complexes conjugated with oligoarginine peptides serve as optical probes for in vivo microvascular imaging
title_short	Phosphorescent Ir(III) complexes conjugated with oligoarginine peptides serve as optical probes for in vivo microvascular imaging
title_full	Phosphorescent Ir(III) complexes conjugated with oligoarginine peptides serve as optical probes for in vivo microvascular imaging
title_fullStr	Phosphorescent Ir(III) complexes conjugated with oligoarginine peptides serve as optical probes for in vivo microvascular imaging
title_full_unstemmed	Phosphorescent Ir(III) complexes conjugated with oligoarginine peptides serve as optical probes for in vivo microvascular imaging
title_sort	phosphorescent ir(iii) complexes conjugated with oligoarginine peptides serve as optical probes for in vivo microvascular imaging
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/b84bc34270044a4284275b59be771f9d
work_keys_str_mv	AT mamiyasukagawa phosphorescentiriiicomplexesconjugatedwitholigoargininepeptidesserveasopticalprobesforinvivomicrovascularimaging AT ayashimada phosphorescentiriiicomplexesconjugatedwitholigoargininepeptidesserveasopticalprobesforinvivomicrovascularimaging AT shuichishiozaki phosphorescentiriiicomplexesconjugatedwitholigoargininepeptidesserveasopticalprobesforinvivomicrovascularimaging AT seijitobita phosphorescentiriiicomplexesconjugatedwitholigoargininepeptidesserveasopticalprobesforinvivomicrovascularimaging AT toshitadayoshihara phosphorescentiriiicomplexesconjugatedwitholigoargininepeptidesserveasopticalprobesforinvivomicrovascularimaging
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Phosphorescent Ir(III) complexes conjugated with oligoarginine peptides serve as optical probes for in vivo microvascular imaging

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