RT-QuIC detection of CWD prion seeding activity in white-tailed deer muscle tissues

Abstract Chronic wasting disease (CWD) is a prion disease circulating in wild and farmed cervid populations throughout North America (United States and Canada), Europe (Finland, Norway, Sweden), and South Korea. CWD is a long-term threat to all cervid populations and to cervid hunting heritage, with...

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Autores principales:	Manci Li, Marc D. Schwabenlander, Gage R. Rowden, Jeremy M. Schefers, Christopher S. Jennelle, Michelle Carstensen, Davis Seelig, Peter A. Larsen
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b84ffcbc904f484a87887a776d6fa48e
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spelling	oai:doaj.org-article:b84ffcbc904f484a87887a776d6fa48e2021-12-02T16:46:34ZRT-QuIC detection of CWD prion seeding activity in white-tailed deer muscle tissues10.1038/s41598-021-96127-82045-2322https://doaj.org/article/b84ffcbc904f484a87887a776d6fa48e2021-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-96127-8https://doaj.org/toc/2045-2322Abstract Chronic wasting disease (CWD) is a prion disease circulating in wild and farmed cervid populations throughout North America (United States and Canada), Europe (Finland, Norway, Sweden), and South Korea. CWD is a long-term threat to all cervid populations and to cervid hunting heritage, with the potential to cause substantial economic losses across multiple sectors. In North America, hunting and farming industries focused on the processing and consumption of white-tailed deer (WTD) venison are particularly vulnerable to CWD prion contamination, as millions of WTD are consumed annually. Real-time quaking-induced conversion (RT-QuIC) is a highly sensitive assay amplifying misfolded CWD prions in vitro and has facilitated CWD prion detection in a variety of tissues and excreta. To date, no study has comprehensively examined CWD prion content across bulk skeletal muscle tissues harvested from individual CWD infected WTD. Here, we use RT-QuIC to characterize prion-seeding activity in a variety of skeletal muscles from both wild and farmed CWD-positive WTD. We successfully detected CWD prions in muscles commonly used for consumption (e.g., backstrap, tenderloin, etc.) as well as within tongue and neck samples of WTD. Our results suggest that CWD prions are distributed across the skeletal muscles of infected WTD. We posit that RT-QuIC will be a useful tool for monitoring CWD prions in venison and that the method (with additional protocol optimization and high-throughput functionality) could be used to reduce and/or prevent CWD prions from entering animal and human food chains.Manci LiMarc D. SchwabenlanderGage R. RowdenJeremy M. SchefersChristopher S. JennelleMichelle CarstensenDavis SeeligPeter A. LarsenNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Manci Li Marc D. Schwabenlander Gage R. Rowden Jeremy M. Schefers Christopher S. Jennelle Michelle Carstensen Davis Seelig Peter A. Larsen RT-QuIC detection of CWD prion seeding activity in white-tailed deer muscle tissues
description	Abstract Chronic wasting disease (CWD) is a prion disease circulating in wild and farmed cervid populations throughout North America (United States and Canada), Europe (Finland, Norway, Sweden), and South Korea. CWD is a long-term threat to all cervid populations and to cervid hunting heritage, with the potential to cause substantial economic losses across multiple sectors. In North America, hunting and farming industries focused on the processing and consumption of white-tailed deer (WTD) venison are particularly vulnerable to CWD prion contamination, as millions of WTD are consumed annually. Real-time quaking-induced conversion (RT-QuIC) is a highly sensitive assay amplifying misfolded CWD prions in vitro and has facilitated CWD prion detection in a variety of tissues and excreta. To date, no study has comprehensively examined CWD prion content across bulk skeletal muscle tissues harvested from individual CWD infected WTD. Here, we use RT-QuIC to characterize prion-seeding activity in a variety of skeletal muscles from both wild and farmed CWD-positive WTD. We successfully detected CWD prions in muscles commonly used for consumption (e.g., backstrap, tenderloin, etc.) as well as within tongue and neck samples of WTD. Our results suggest that CWD prions are distributed across the skeletal muscles of infected WTD. We posit that RT-QuIC will be a useful tool for monitoring CWD prions in venison and that the method (with additional protocol optimization and high-throughput functionality) could be used to reduce and/or prevent CWD prions from entering animal and human food chains.
format	article
author	Manci Li Marc D. Schwabenlander Gage R. Rowden Jeremy M. Schefers Christopher S. Jennelle Michelle Carstensen Davis Seelig Peter A. Larsen
author_facet	Manci Li Marc D. Schwabenlander Gage R. Rowden Jeremy M. Schefers Christopher S. Jennelle Michelle Carstensen Davis Seelig Peter A. Larsen
author_sort	Manci Li
title	RT-QuIC detection of CWD prion seeding activity in white-tailed deer muscle tissues
title_short	RT-QuIC detection of CWD prion seeding activity in white-tailed deer muscle tissues
title_full	RT-QuIC detection of CWD prion seeding activity in white-tailed deer muscle tissues
title_fullStr	RT-QuIC detection of CWD prion seeding activity in white-tailed deer muscle tissues
title_full_unstemmed	RT-QuIC detection of CWD prion seeding activity in white-tailed deer muscle tissues
title_sort	rt-quic detection of cwd prion seeding activity in white-tailed deer muscle tissues
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/b84ffcbc904f484a87887a776d6fa48e
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RT-QuIC detection of CWD prion seeding activity in white-tailed deer muscle tissues

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