Genome-Wide Analysis of the Glutathione S-Transferase (GST) Genes and Functional Identification of <i>MdGSTU12</i> Reveals the Involvement in the Regulation of Anthocyanin Accumulation in Apple

Anthocyanins have essential biological functions, affecting the development of horticultural production. They are synthesized in the cytoplasm through flavonoid metabolic pathways and finally transported into vacuoles for storage. Plant glutathione S-transferases (GSTs) are multifunctional enzymes i...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Yu-Wen Zhao, Chu-Kun Wang, Xiao-Yu Huang, Da-Gang Hu
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	apple anthocyanin glutathione S-transferase (GST) gene family <i>MdGSTU12</i> Genetics QH426-470
Acceso en línea:	https://doaj.org/article/b85028397eb74d4f905c226d30e9960f
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:b85028397eb74d4f905c226d30e9960f
record_format	dspace
spelling	oai:doaj.org-article:b85028397eb74d4f905c226d30e9960f2021-11-25T17:41:21ZGenome-Wide Analysis of the Glutathione S-Transferase (GST) Genes and Functional Identification of <i>MdGSTU12</i> Reveals the Involvement in the Regulation of Anthocyanin Accumulation in Apple10.3390/genes121117332073-4425https://doaj.org/article/b85028397eb74d4f905c226d30e9960f2021-10-01T00:00:00Zhttps://www.mdpi.com/2073-4425/12/11/1733https://doaj.org/toc/2073-4425Anthocyanins have essential biological functions, affecting the development of horticultural production. They are synthesized in the cytoplasm through flavonoid metabolic pathways and finally transported into vacuoles for storage. Plant glutathione S-transferases (GSTs) are multifunctional enzymes involved in anthocyanin transportation. In this study, we identified 38 GSTs from the apple (<i>Malus domestica</i>) genome (HFTH1 Whole Genome v1.0) based on the sequence similarity with the GST family proteins of <i>Arabidopsis</i>. These <i>MdGST</i> genes could be grouped into nine chief subclasses: U, F, L, Z, T, GHR, EF1Bγ, TCHQD, and DHAR. The structures, motifs, three-dimensional models, and chromosomal distribution of <i>MdGST</i> genes were further analyzed. Elements which are responsive for some hormones and stress, and others that involve genes related to flavonoid biosynthesis were forecast in the promoter of <i>MdGST</i>. In addition, we identified 32 orthologous gene pairs between apple and <i>Arabidopsis</i>. These genes indicated that numerous apple and <i>Arabidopsis</i> counterparts appeared to be derived from a common ancestor. Amongst the 38 <i>MdGST</i> genes, <i>MdGSTU12</i> was considerably correlated with anthocyanin variation in terms of extracting expression profiles from reported. Finally, further functional identification in apple transgenic calli and subcellular localization confirmed that <i>MdGSTU12</i> was of great significance in anthocyanin accumulation in apple.Yu-Wen ZhaoChu-Kun WangXiao-Yu HuangDa-Gang HuMDPI AGarticleappleanthocyaninglutathione S-transferase (GST)gene family<i>MdGSTU12</i>GeneticsQH426-470ENGenes, Vol 12, Iss 1733, p 1733 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	apple anthocyanin glutathione S-transferase (GST) gene family <i>MdGSTU12</i> Genetics QH426-470
spellingShingle	apple anthocyanin glutathione S-transferase (GST) gene family <i>MdGSTU12</i> Genetics QH426-470 Yu-Wen Zhao Chu-Kun Wang Xiao-Yu Huang Da-Gang Hu Genome-Wide Analysis of the Glutathione S-Transferase (GST) Genes and Functional Identification of <i>MdGSTU12</i> Reveals the Involvement in the Regulation of Anthocyanin Accumulation in Apple
description	Anthocyanins have essential biological functions, affecting the development of horticultural production. They are synthesized in the cytoplasm through flavonoid metabolic pathways and finally transported into vacuoles for storage. Plant glutathione S-transferases (GSTs) are multifunctional enzymes involved in anthocyanin transportation. In this study, we identified 38 GSTs from the apple (<i>Malus domestica</i>) genome (HFTH1 Whole Genome v1.0) based on the sequence similarity with the GST family proteins of <i>Arabidopsis</i>. These <i>MdGST</i> genes could be grouped into nine chief subclasses: U, F, L, Z, T, GHR, EF1Bγ, TCHQD, and DHAR. The structures, motifs, three-dimensional models, and chromosomal distribution of <i>MdGST</i> genes were further analyzed. Elements which are responsive for some hormones and stress, and others that involve genes related to flavonoid biosynthesis were forecast in the promoter of <i>MdGST</i>. In addition, we identified 32 orthologous gene pairs between apple and <i>Arabidopsis</i>. These genes indicated that numerous apple and <i>Arabidopsis</i> counterparts appeared to be derived from a common ancestor. Amongst the 38 <i>MdGST</i> genes, <i>MdGSTU12</i> was considerably correlated with anthocyanin variation in terms of extracting expression profiles from reported. Finally, further functional identification in apple transgenic calli and subcellular localization confirmed that <i>MdGSTU12</i> was of great significance in anthocyanin accumulation in apple.
format	article
author	Yu-Wen Zhao Chu-Kun Wang Xiao-Yu Huang Da-Gang Hu
author_facet	Yu-Wen Zhao Chu-Kun Wang Xiao-Yu Huang Da-Gang Hu
author_sort	Yu-Wen Zhao
title	Genome-Wide Analysis of the Glutathione S-Transferase (GST) Genes and Functional Identification of <i>MdGSTU12</i> Reveals the Involvement in the Regulation of Anthocyanin Accumulation in Apple
title_short	Genome-Wide Analysis of the Glutathione S-Transferase (GST) Genes and Functional Identification of <i>MdGSTU12</i> Reveals the Involvement in the Regulation of Anthocyanin Accumulation in Apple
title_full	Genome-Wide Analysis of the Glutathione S-Transferase (GST) Genes and Functional Identification of <i>MdGSTU12</i> Reveals the Involvement in the Regulation of Anthocyanin Accumulation in Apple
title_fullStr	Genome-Wide Analysis of the Glutathione S-Transferase (GST) Genes and Functional Identification of <i>MdGSTU12</i> Reveals the Involvement in the Regulation of Anthocyanin Accumulation in Apple
title_full_unstemmed	Genome-Wide Analysis of the Glutathione S-Transferase (GST) Genes and Functional Identification of <i>MdGSTU12</i> Reveals the Involvement in the Regulation of Anthocyanin Accumulation in Apple
title_sort	genome-wide analysis of the glutathione s-transferase (gst) genes and functional identification of <i>mdgstu12</i> reveals the involvement in the regulation of anthocyanin accumulation in apple
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/b85028397eb74d4f905c226d30e9960f
work_keys_str_mv	AT yuwenzhao genomewideanalysisoftheglutathionestransferasegstgenesandfunctionalidentificationofimdgstu12irevealstheinvolvementintheregulationofanthocyaninaccumulationinapple AT chukunwang genomewideanalysisoftheglutathionestransferasegstgenesandfunctionalidentificationofimdgstu12irevealstheinvolvementintheregulationofanthocyaninaccumulationinapple AT xiaoyuhuang genomewideanalysisoftheglutathionestransferasegstgenesandfunctionalidentificationofimdgstu12irevealstheinvolvementintheregulationofanthocyaninaccumulationinapple AT daganghu genomewideanalysisoftheglutathionestransferasegstgenesandfunctionalidentificationofimdgstu12irevealstheinvolvementintheregulationofanthocyaninaccumulationinapple
_version_	1718412127580979200

Genome-Wide Analysis of the Glutathione S-Transferase (GST) Genes and Functional Identification of <i>MdGSTU12</i> Reveals the Involvement in the Regulation of Anthocyanin Accumulation in Apple

Ejemplares similares