Bacterial diversity and enzymatic activity in a soil recently treated with tebuconazole

Tebuconazole is one of the most commonly used active substances from the group of triazoles, that exhibits high efficacy in plant protection against fungal diseases. It’s too frequent use may, however, pose risk to soil ecosystems, leading to changes in their biological diversity. This study was aim...

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Autores principales:	Małgorzata Baćmaga, Jadwiga Wyszkowska, Jan Kucharski
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Diversity of bacteria Enzymes activity NGS Soil Tebuconazole Ecology QH540-549.5
Acceso en línea:	https://doaj.org/article/b85a99fe357146ef9095542c7333f4ec
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spelling	oai:doaj.org-article:b85a99fe357146ef9095542c7333f4ec2021-12-01T04:44:38ZBacterial diversity and enzymatic activity in a soil recently treated with tebuconazole1470-160X10.1016/j.ecolind.2021.107373https://doaj.org/article/b85a99fe357146ef9095542c7333f4ec2021-04-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S1470160X21000388https://doaj.org/toc/1470-160XTebuconazole is one of the most commonly used active substances from the group of triazoles, that exhibits high efficacy in plant protection against fungal diseases. It’s too frequent use may, however, pose risk to soil ecosystems, leading to changes in their biological diversity. This study was aimed at evaluating the effect of tebuconazole on population numbers, diversity, and structure of bacterial communities, and on the enzymatic activity of soil. Tebuconazole was introduced into the soil (sandy loam with pH 7.0) in the following doses in mg kg−1 DM (dry matter) of soil: 0.00 (C), 0.02 (O), and 10.0 (T). It caused changes in the population numbers of diversity of bacteria as well as in the biochemical activity of soil. It stimulated the proliferation of organotrophic bacteria and inhibited that of actinobacteria. The r-strategists were found to predominate among both the organotrophs and actinobacteria in the soil with tebuconazole addition. The amplicon sequencing of the 16S encoding gene (SSU rRNA) demonstrated tebuconazole to elicit changes in the structure of bacterial communities. In all soil samples, the prevailing taxon at the Phylum level turned out to be Proteobacteria, followed by Firmicutes and Actinobacteria, whereas the lowest counts were noted for Verrucomicrobia. The relative abundance of the bacteria at the Phylum level decreased in the soil under the influence of tebuconazole. The most sensitive to the effect of tebuconazole applied in a dose of 10.0 mg kg−1 turned out to be Acidobacteria, whereas the most resistant were Verrucomicrobia. The predominating taxon at the class level was Alphaproteobacteria, followed by Bacilli. Tebuconazole ensured favorable conditions for the development of bacteria from Kaistobacter and Bacillus genera as their OTUs were the highest in the soil treated with tebuconazole at 10.0 mg kg−1. In turn, Phenylobacterium and Rhodoplanes were sensitive to soil treatment with tebuconazole at doses of 0.02 mg kg−1 and 10.0 mg kg−1. Tebuconazole proved to be a strong inhibitor of urease and catalase activities, while in turn it enhanced activities of dehydrogenases, acid phosphatase, alkaline phosphatase, and arylsulfatase.Małgorzata BaćmagaJadwiga WyszkowskaJan KucharskiElsevierarticleDiversity of bacteriaEnzymes activityNGSSoilTebuconazoleEcologyQH540-549.5ENEcological Indicators, Vol 123, Iss , Pp 107373- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Diversity of bacteria Enzymes activity NGS Soil Tebuconazole Ecology QH540-549.5
spellingShingle	Diversity of bacteria Enzymes activity NGS Soil Tebuconazole Ecology QH540-549.5 Małgorzata Baćmaga Jadwiga Wyszkowska Jan Kucharski Bacterial diversity and enzymatic activity in a soil recently treated with tebuconazole
description	Tebuconazole is one of the most commonly used active substances from the group of triazoles, that exhibits high efficacy in plant protection against fungal diseases. It’s too frequent use may, however, pose risk to soil ecosystems, leading to changes in their biological diversity. This study was aimed at evaluating the effect of tebuconazole on population numbers, diversity, and structure of bacterial communities, and on the enzymatic activity of soil. Tebuconazole was introduced into the soil (sandy loam with pH 7.0) in the following doses in mg kg−1 DM (dry matter) of soil: 0.00 (C), 0.02 (O), and 10.0 (T). It caused changes in the population numbers of diversity of bacteria as well as in the biochemical activity of soil. It stimulated the proliferation of organotrophic bacteria and inhibited that of actinobacteria. The r-strategists were found to predominate among both the organotrophs and actinobacteria in the soil with tebuconazole addition. The amplicon sequencing of the 16S encoding gene (SSU rRNA) demonstrated tebuconazole to elicit changes in the structure of bacterial communities. In all soil samples, the prevailing taxon at the Phylum level turned out to be Proteobacteria, followed by Firmicutes and Actinobacteria, whereas the lowest counts were noted for Verrucomicrobia. The relative abundance of the bacteria at the Phylum level decreased in the soil under the influence of tebuconazole. The most sensitive to the effect of tebuconazole applied in a dose of 10.0 mg kg−1 turned out to be Acidobacteria, whereas the most resistant were Verrucomicrobia. The predominating taxon at the class level was Alphaproteobacteria, followed by Bacilli. Tebuconazole ensured favorable conditions for the development of bacteria from Kaistobacter and Bacillus genera as their OTUs were the highest in the soil treated with tebuconazole at 10.0 mg kg−1. In turn, Phenylobacterium and Rhodoplanes were sensitive to soil treatment with tebuconazole at doses of 0.02 mg kg−1 and 10.0 mg kg−1. Tebuconazole proved to be a strong inhibitor of urease and catalase activities, while in turn it enhanced activities of dehydrogenases, acid phosphatase, alkaline phosphatase, and arylsulfatase.
format	article
author	Małgorzata Baćmaga Jadwiga Wyszkowska Jan Kucharski
author_facet	Małgorzata Baćmaga Jadwiga Wyszkowska Jan Kucharski
author_sort	Małgorzata Baćmaga
title	Bacterial diversity and enzymatic activity in a soil recently treated with tebuconazole
title_short	Bacterial diversity and enzymatic activity in a soil recently treated with tebuconazole
title_full	Bacterial diversity and enzymatic activity in a soil recently treated with tebuconazole
title_fullStr	Bacterial diversity and enzymatic activity in a soil recently treated with tebuconazole
title_full_unstemmed	Bacterial diversity and enzymatic activity in a soil recently treated with tebuconazole
title_sort	bacterial diversity and enzymatic activity in a soil recently treated with tebuconazole
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/b85a99fe357146ef9095542c7333f4ec
work_keys_str_mv	AT małgorzatabacmaga bacterialdiversityandenzymaticactivityinasoilrecentlytreatedwithtebuconazole AT jadwigawyszkowska bacterialdiversityandenzymaticactivityinasoilrecentlytreatedwithtebuconazole AT jankucharski bacterialdiversityandenzymaticactivityinasoilrecentlytreatedwithtebuconazole
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Bacterial diversity and enzymatic activity in a soil recently treated with tebuconazole

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