Arabidopsis RPT2a, 19S proteasome subunit, regulates gene silencing via DNA methylation.

The ubiquitin/proteasome pathway plays a crucial role in many biological processes. Here we report a novel role for the Arabidopsis 19S proteasome subunit RPT2a in regulating gene activity at the transcriptional level via DNA methylation. Knockout mutation of the RPT2a gene did not alter global prot...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Kaori Sako, Yuko Maki, Tomoyuki Kanai, Eriko Kato, Shugo Maekawa, Shigetaka Yasuda, Takeo Sato, Masaaki K Watahiki, Junji Yamaguchi
Formato: article
Lenguaje:EN
Publicado: Public Library of Science (PLoS) 2012
Materias:
R
Q
Acceso en línea:https://doaj.org/article/b8659f1565104e50aa25433b5e2e3ac9
Etiquetas: Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
id oai:doaj.org-article:b8659f1565104e50aa25433b5e2e3ac9
record_format dspace
spelling oai:doaj.org-article:b8659f1565104e50aa25433b5e2e3ac92021-11-18T07:18:30ZArabidopsis RPT2a, 19S proteasome subunit, regulates gene silencing via DNA methylation.1932-620310.1371/journal.pone.0037086https://doaj.org/article/b8659f1565104e50aa25433b5e2e3ac92012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22615900/?tool=EBIhttps://doaj.org/toc/1932-6203The ubiquitin/proteasome pathway plays a crucial role in many biological processes. Here we report a novel role for the Arabidopsis 19S proteasome subunit RPT2a in regulating gene activity at the transcriptional level via DNA methylation. Knockout mutation of the RPT2a gene did not alter global protein levels; however, the transcriptional activities of reporter transgenes were severely reduced compared to those in the wild type. This transcriptional gene silencing (TGS) was observed for transgenes under control of either the constitutive CaMV 35S promoter or the cold-inducible RD29A promoter. Bisulfite sequencing analysis revealed that both the transgene and endogenous RD29A promoter regions were hypermethylated at CG and non-CG contexts in the rpt2a mutant. Moreover, the TGS of transgenes driven by the CaMV 35S promoters was released by treatment with the DNA methylation inhibitor 5-aza-2'-deoxycytidine, but not by application of the inhibitor of histone deacetylase Trichostatin A. Genetic crosses with the DNA methyltransferase met1 single or drm1drm2cmt3 triple mutants also resulted in a release of CaMV 35S transgene TGS in the rpt2a mutant background. Increased methylation was also found at transposon sequences, suggesting that the 19S proteasome containing AtRPT2a negatively regulates TGS at transgenes and at specific endogenous genes through DNA methylation.Kaori SakoYuko MakiTomoyuki KanaiEriko KatoShugo MaekawaShigetaka YasudaTakeo SatoMasaaki K WatahikiJunji YamaguchiPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 5, p e37086 (2012)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Kaori Sako
Yuko Maki
Tomoyuki Kanai
Eriko Kato
Shugo Maekawa
Shigetaka Yasuda
Takeo Sato
Masaaki K Watahiki
Junji Yamaguchi
Arabidopsis RPT2a, 19S proteasome subunit, regulates gene silencing via DNA methylation.
description The ubiquitin/proteasome pathway plays a crucial role in many biological processes. Here we report a novel role for the Arabidopsis 19S proteasome subunit RPT2a in regulating gene activity at the transcriptional level via DNA methylation. Knockout mutation of the RPT2a gene did not alter global protein levels; however, the transcriptional activities of reporter transgenes were severely reduced compared to those in the wild type. This transcriptional gene silencing (TGS) was observed for transgenes under control of either the constitutive CaMV 35S promoter or the cold-inducible RD29A promoter. Bisulfite sequencing analysis revealed that both the transgene and endogenous RD29A promoter regions were hypermethylated at CG and non-CG contexts in the rpt2a mutant. Moreover, the TGS of transgenes driven by the CaMV 35S promoters was released by treatment with the DNA methylation inhibitor 5-aza-2'-deoxycytidine, but not by application of the inhibitor of histone deacetylase Trichostatin A. Genetic crosses with the DNA methyltransferase met1 single or drm1drm2cmt3 triple mutants also resulted in a release of CaMV 35S transgene TGS in the rpt2a mutant background. Increased methylation was also found at transposon sequences, suggesting that the 19S proteasome containing AtRPT2a negatively regulates TGS at transgenes and at specific endogenous genes through DNA methylation.
format article
author Kaori Sako
Yuko Maki
Tomoyuki Kanai
Eriko Kato
Shugo Maekawa
Shigetaka Yasuda
Takeo Sato
Masaaki K Watahiki
Junji Yamaguchi
author_facet Kaori Sako
Yuko Maki
Tomoyuki Kanai
Eriko Kato
Shugo Maekawa
Shigetaka Yasuda
Takeo Sato
Masaaki K Watahiki
Junji Yamaguchi
author_sort Kaori Sako
title Arabidopsis RPT2a, 19S proteasome subunit, regulates gene silencing via DNA methylation.
title_short Arabidopsis RPT2a, 19S proteasome subunit, regulates gene silencing via DNA methylation.
title_full Arabidopsis RPT2a, 19S proteasome subunit, regulates gene silencing via DNA methylation.
title_fullStr Arabidopsis RPT2a, 19S proteasome subunit, regulates gene silencing via DNA methylation.
title_full_unstemmed Arabidopsis RPT2a, 19S proteasome subunit, regulates gene silencing via DNA methylation.
title_sort arabidopsis rpt2a, 19s proteasome subunit, regulates gene silencing via dna methylation.
publisher Public Library of Science (PLoS)
publishDate 2012
url https://doaj.org/article/b8659f1565104e50aa25433b5e2e3ac9
work_keys_str_mv AT kaorisako arabidopsisrpt2a19sproteasomesubunitregulatesgenesilencingviadnamethylation
AT yukomaki arabidopsisrpt2a19sproteasomesubunitregulatesgenesilencingviadnamethylation
AT tomoyukikanai arabidopsisrpt2a19sproteasomesubunitregulatesgenesilencingviadnamethylation
AT erikokato arabidopsisrpt2a19sproteasomesubunitregulatesgenesilencingviadnamethylation
AT shugomaekawa arabidopsisrpt2a19sproteasomesubunitregulatesgenesilencingviadnamethylation
AT shigetakayasuda arabidopsisrpt2a19sproteasomesubunitregulatesgenesilencingviadnamethylation
AT takeosato arabidopsisrpt2a19sproteasomesubunitregulatesgenesilencingviadnamethylation
AT masaakikwatahiki arabidopsisrpt2a19sproteasomesubunitregulatesgenesilencingviadnamethylation
AT junjiyamaguchi arabidopsisrpt2a19sproteasomesubunitregulatesgenesilencingviadnamethylation
_version_ 1718423689295298560