Identification of potentially pathogenic bacteria from tilapia (Oreochromis niloticus) and channel catfish (Clarias batrachus) culture in Samarinda, East Kalimantan, Indonesia

Hardi EH, Nugroho RA, Saptiani G, Sarinah R, Anggraidini M, Mawardi M. 2018. Identification of potentially pathogenic bacteria from tilapia (Oreochromis niloticus) and channel catfish (Clarias batrachus) culture in Samarinda, East Kalimantan, Indonesia. Biodiversitas 19: 480-488. This research was c...

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Autores principales:	ESTI HANDAYANI HARDI, RUDY AGUNG NUGROHO, GINA SAPTIANI, RIA SARINAH, MAULINA AGRIANDINI, MIRA MAWARDI
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2018
Materias:	bacteria colony contaminant channel catfish pathogen bacteria tilapia Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/b86917a2a3014544a11886d67e4a0c5d
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spelling	oai:doaj.org-article:b86917a2a3014544a11886d67e4a0c5d2021-11-16T14:01:41ZIdentification of potentially pathogenic bacteria from tilapia (Oreochromis niloticus) and channel catfish (Clarias batrachus) culture in Samarinda, East Kalimantan, Indonesia1412-033X2085-472210.13057/biodiv/d190215https://doaj.org/article/b86917a2a3014544a11886d67e4a0c5d2018-03-01T00:00:00Zhttps://smujo.id/biodiv/article/view/2474https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Hardi EH, Nugroho RA, Saptiani G, Sarinah R, Anggraidini M, Mawardi M. 2018. Identification of potentially pathogenic bacteria from tilapia (Oreochromis niloticus) and channel catfish (Clarias batrachus) culture in Samarinda, East Kalimantan, Indonesia. Biodiversitas 19: 480-488. This research was conducted to isolate, identify, and characterize pathogenic bacteria from feces and water of tilapia (Oreochromis niloticus) and channel catfish (Clarias batrachus) culture from two selected locations in Samarinda, East Kalimantan, Indonesia. Bacteria were cultured and isolated on NA, TSA, and BHIA media at 30oC for 24 h. Colonies of the isolated bacteria were characterized morphologically in terms of their shape, chromogenesis, edge, and size. Antibiotic sensitivity test on each bacterial isolate was completed using inhibition zone tests. Commercial antibiotics used in this research were nitrofurantoin, ciprofloxacin, oxytetracycline, chloramphenicol, nalidixic acid, gentamicin, and norfloxacin. Koch’s postulates test was done by intraperitoneal injection of bacterial suspension to tilapia (15 g weight) at 103-109 CFU mL-1 in triplicates to determine the pathogenicity of each bacterium. Overall, there were 37 isolates obtained from different sources and growth media that belonged to 14 species: Acinetobacter calcoaceticus (1 isolate), Aerococcus urinae (2 isolates), Aerococcus viridans (1 isolate), Aeromonas hydrophila (1 isolate), Citrobacter freundii (5 isolates), Enterobacter amnigenus (2 isolate), Enterobacter cloacae (4 isolates), Escherichia coli (3 isolates), Listeria sp. (1 isolate), Niseria sp. (4 isolates), Pantoea spp. (1 isolate), Pseudomonas aeruginosa (1 isolate), Staphylococcus aureus (9 isolates), and Streptococcus iniae (2 isolates). Sixteen of the isolates were grown in BHIA medium, 12 isolates in TSA medium and 9 isolates in NA medium. The highest mortality was found in tilapia injected with Enterobacter sp., Listeria sp. and Streptococcus sp. at a density of 109 CFU mL-1. However, the number of bacteria causing mortality in fish was approximately 104-108 CFU mL-1. All bacteria detected in the tilapia and channel catfish cultures were also known as putative pathogens in human.ESTI HANDAYANI HARDIRUDY AGUNG NUGROHOGINA SAPTIANIRIA SARINAHMAULINA AGRIANDINIMIRA MAWARDIMBI & UNS Soloarticlebacteria colonycontaminantchannel catfishpathogen bacteriatilapiaBiology (General)QH301-705.5ENBiodiversitas, Vol 19, Iss 2, Pp 480-488 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	bacteria colony contaminant channel catfish pathogen bacteria tilapia Biology (General) QH301-705.5
spellingShingle	bacteria colony contaminant channel catfish pathogen bacteria tilapia Biology (General) QH301-705.5 ESTI HANDAYANI HARDI RUDY AGUNG NUGROHO GINA SAPTIANI RIA SARINAH MAULINA AGRIANDINI MIRA MAWARDI Identification of potentially pathogenic bacteria from tilapia (Oreochromis niloticus) and channel catfish (Clarias batrachus) culture in Samarinda, East Kalimantan, Indonesia
description	Hardi EH, Nugroho RA, Saptiani G, Sarinah R, Anggraidini M, Mawardi M. 2018. Identification of potentially pathogenic bacteria from tilapia (Oreochromis niloticus) and channel catfish (Clarias batrachus) culture in Samarinda, East Kalimantan, Indonesia. Biodiversitas 19: 480-488. This research was conducted to isolate, identify, and characterize pathogenic bacteria from feces and water of tilapia (Oreochromis niloticus) and channel catfish (Clarias batrachus) culture from two selected locations in Samarinda, East Kalimantan, Indonesia. Bacteria were cultured and isolated on NA, TSA, and BHIA media at 30oC for 24 h. Colonies of the isolated bacteria were characterized morphologically in terms of their shape, chromogenesis, edge, and size. Antibiotic sensitivity test on each bacterial isolate was completed using inhibition zone tests. Commercial antibiotics used in this research were nitrofurantoin, ciprofloxacin, oxytetracycline, chloramphenicol, nalidixic acid, gentamicin, and norfloxacin. Koch’s postulates test was done by intraperitoneal injection of bacterial suspension to tilapia (15 g weight) at 103-109 CFU mL-1 in triplicates to determine the pathogenicity of each bacterium. Overall, there were 37 isolates obtained from different sources and growth media that belonged to 14 species: Acinetobacter calcoaceticus (1 isolate), Aerococcus urinae (2 isolates), Aerococcus viridans (1 isolate), Aeromonas hydrophila (1 isolate), Citrobacter freundii (5 isolates), Enterobacter amnigenus (2 isolate), Enterobacter cloacae (4 isolates), Escherichia coli (3 isolates), Listeria sp. (1 isolate), Niseria sp. (4 isolates), Pantoea spp. (1 isolate), Pseudomonas aeruginosa (1 isolate), Staphylococcus aureus (9 isolates), and Streptococcus iniae (2 isolates). Sixteen of the isolates were grown in BHIA medium, 12 isolates in TSA medium and 9 isolates in NA medium. The highest mortality was found in tilapia injected with Enterobacter sp., Listeria sp. and Streptococcus sp. at a density of 109 CFU mL-1. However, the number of bacteria causing mortality in fish was approximately 104-108 CFU mL-1. All bacteria detected in the tilapia and channel catfish cultures were also known as putative pathogens in human.
format	article
author	ESTI HANDAYANI HARDI RUDY AGUNG NUGROHO GINA SAPTIANI RIA SARINAH MAULINA AGRIANDINI MIRA MAWARDI
author_facet	ESTI HANDAYANI HARDI RUDY AGUNG NUGROHO GINA SAPTIANI RIA SARINAH MAULINA AGRIANDINI MIRA MAWARDI
author_sort	ESTI HANDAYANI HARDI
title	Identification of potentially pathogenic bacteria from tilapia (Oreochromis niloticus) and channel catfish (Clarias batrachus) culture in Samarinda, East Kalimantan, Indonesia
title_short	Identification of potentially pathogenic bacteria from tilapia (Oreochromis niloticus) and channel catfish (Clarias batrachus) culture in Samarinda, East Kalimantan, Indonesia
title_full	Identification of potentially pathogenic bacteria from tilapia (Oreochromis niloticus) and channel catfish (Clarias batrachus) culture in Samarinda, East Kalimantan, Indonesia
title_fullStr	Identification of potentially pathogenic bacteria from tilapia (Oreochromis niloticus) and channel catfish (Clarias batrachus) culture in Samarinda, East Kalimantan, Indonesia
title_full_unstemmed	Identification of potentially pathogenic bacteria from tilapia (Oreochromis niloticus) and channel catfish (Clarias batrachus) culture in Samarinda, East Kalimantan, Indonesia
title_sort	identification of potentially pathogenic bacteria from tilapia (oreochromis niloticus) and channel catfish (clarias batrachus) culture in samarinda, east kalimantan, indonesia
publisher	MBI & UNS Solo
publishDate	2018
url	https://doaj.org/article/b86917a2a3014544a11886d67e4a0c5d
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Identification of potentially pathogenic bacteria from tilapia (Oreochromis niloticus) and channel catfish (Clarias batrachus) culture in Samarinda, East Kalimantan, Indonesia

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