High prevalence of both humoral and cellular immunity to Zaire ebolavirus among rural populations in Gabon.

To better understand Zaire ebolavirus (ZEBOV) circulation and transmission to humans, we conducted a large serological survey of rural populations in Gabon, a country characterized by both epidemic and non epidemic regions. The survey lasted three years and covered 4,349 individuals from 220 randoml...

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Autores principales:	Pierre Becquart, Nadia Wauquier, Tanel Mahlakõiv, Dieudonné Nkoghe, Cindy Padilla, Marc Souris, Benjamin Ollomo, Jean-Paul Gonzalez, Xavier De Lamballerie, Mirdad Kazanji, Eric M Leroy
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2010
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b86b5bb5197a4904b017d963bb2724a3
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spelling	oai:doaj.org-article:b86b5bb5197a4904b017d963bb2724a32021-11-25T06:26:00ZHigh prevalence of both humoral and cellular immunity to Zaire ebolavirus among rural populations in Gabon.1932-620310.1371/journal.pone.0009126https://doaj.org/article/b86b5bb5197a4904b017d963bb2724a32010-02-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/20161740/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203To better understand Zaire ebolavirus (ZEBOV) circulation and transmission to humans, we conducted a large serological survey of rural populations in Gabon, a country characterized by both epidemic and non epidemic regions. The survey lasted three years and covered 4,349 individuals from 220 randomly selected villages, representing 10.7% of all villages in Gabon. Using a sensitive and specific ELISA method, we found a ZEBOV-specific IgG seroprevalence of 15.3% overall, the highest ever reported. The seroprevalence rate was significantly higher in forested areas (19.4%) than in other ecosystems, namely grassland (12.4%), savannah (10.5%), and lakeland (2.7%). No other risk factors for seropositivity were found. The specificity of anti-ZEBOV IgG was confirmed by Western blot in 138 individuals, and CD8 T cells from seven IgG+ individuals were shown to produce IFN-gamma after ZEBOV stimulation. Together, these findings show that a large fraction of the human population living in forested areas of Gabon has both humoral and cellular immunity to ZEBOV. In the absence of identified risk factors, the high prevalence of "immune" persons suggests a common source of human exposure such as fruits contaminated by bat saliva. These findings provide significant new insights into ZEBOV circulation and human exposure, and raise important questions as to the human pathogenicity of ZEBOV and the existence of natural protective immunization.Pierre BecquartNadia WauquierTanel MahlakõivDieudonné NkogheCindy PadillaMarc SourisBenjamin OllomoJean-Paul GonzalezXavier De LamballerieMirdad KazanjiEric M LeroyPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 5, Iss 2, p e9126 (2010)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Pierre Becquart Nadia Wauquier Tanel Mahlakõiv Dieudonné Nkoghe Cindy Padilla Marc Souris Benjamin Ollomo Jean-Paul Gonzalez Xavier De Lamballerie Mirdad Kazanji Eric M Leroy High prevalence of both humoral and cellular immunity to Zaire ebolavirus among rural populations in Gabon.
description	To better understand Zaire ebolavirus (ZEBOV) circulation and transmission to humans, we conducted a large serological survey of rural populations in Gabon, a country characterized by both epidemic and non epidemic regions. The survey lasted three years and covered 4,349 individuals from 220 randomly selected villages, representing 10.7% of all villages in Gabon. Using a sensitive and specific ELISA method, we found a ZEBOV-specific IgG seroprevalence of 15.3% overall, the highest ever reported. The seroprevalence rate was significantly higher in forested areas (19.4%) than in other ecosystems, namely grassland (12.4%), savannah (10.5%), and lakeland (2.7%). No other risk factors for seropositivity were found. The specificity of anti-ZEBOV IgG was confirmed by Western blot in 138 individuals, and CD8 T cells from seven IgG+ individuals were shown to produce IFN-gamma after ZEBOV stimulation. Together, these findings show that a large fraction of the human population living in forested areas of Gabon has both humoral and cellular immunity to ZEBOV. In the absence of identified risk factors, the high prevalence of "immune" persons suggests a common source of human exposure such as fruits contaminated by bat saliva. These findings provide significant new insights into ZEBOV circulation and human exposure, and raise important questions as to the human pathogenicity of ZEBOV and the existence of natural protective immunization.
format	article
author	Pierre Becquart Nadia Wauquier Tanel Mahlakõiv Dieudonné Nkoghe Cindy Padilla Marc Souris Benjamin Ollomo Jean-Paul Gonzalez Xavier De Lamballerie Mirdad Kazanji Eric M Leroy
author_facet	Pierre Becquart Nadia Wauquier Tanel Mahlakõiv Dieudonné Nkoghe Cindy Padilla Marc Souris Benjamin Ollomo Jean-Paul Gonzalez Xavier De Lamballerie Mirdad Kazanji Eric M Leroy
author_sort	Pierre Becquart
title	High prevalence of both humoral and cellular immunity to Zaire ebolavirus among rural populations in Gabon.
title_short	High prevalence of both humoral and cellular immunity to Zaire ebolavirus among rural populations in Gabon.
title_full	High prevalence of both humoral and cellular immunity to Zaire ebolavirus among rural populations in Gabon.
title_fullStr	High prevalence of both humoral and cellular immunity to Zaire ebolavirus among rural populations in Gabon.
title_full_unstemmed	High prevalence of both humoral and cellular immunity to Zaire ebolavirus among rural populations in Gabon.
title_sort	high prevalence of both humoral and cellular immunity to zaire ebolavirus among rural populations in gabon.
publisher	Public Library of Science (PLoS)
publishDate	2010
url	https://doaj.org/article/b86b5bb5197a4904b017d963bb2724a3
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High prevalence of both humoral and cellular immunity to Zaire ebolavirus among rural populations in Gabon.

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