Involvement of Monocyte Subsets in the Immunopathology of Giant Cell Arteritis

Abstract Monocytes/macrophages are critical in systemic and local inflammation in giant cell arteritis (GCA) and possibly in clinically overlapping polymyalgia rheumatica (PMR). Therefore, we aimed to understand the contribution of monocyte subsets and the CX3CR1-CX3CL1 and CCR2-CCL2 migratory pathw...

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Autores principales:	Yannick van Sleen, Qi Wang, Kornelis S. M. van der Geest, Johanna Westra, Wayel H. Abdulahad, Peter Heeringa, Annemieke M. H. Boots, Elisabeth Brouwer
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b86d4cd688644cae93d608708ac0a7e4
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spelling	oai:doaj.org-article:b86d4cd688644cae93d608708ac0a7e42021-12-02T12:30:17ZInvolvement of Monocyte Subsets in the Immunopathology of Giant Cell Arteritis10.1038/s41598-017-06826-42045-2322https://doaj.org/article/b86d4cd688644cae93d608708ac0a7e42017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-06826-4https://doaj.org/toc/2045-2322Abstract Monocytes/macrophages are critical in systemic and local inflammation in giant cell arteritis (GCA) and possibly in clinically overlapping polymyalgia rheumatica (PMR). Therefore, we aimed to understand the contribution of monocyte subsets and the CX3CR1-CX3CL1 and CCR2-CCL2 migratory pathways, to the pathology of GCA. Peripheral blood monocytes were enumerated in samples from newly-diagnosed, untreated GCA and PMR patients and after prednisone-induced remission. The distribution of classical (CD14brightCD16neg) and the more pro-inflammatory, intermediate (CD14brightCD16+) and non-classical (CD14dimCD16+) monocyte subsets was analysed by flow cytometry. The phenotype of macrophages in temporal artery biopsies (TABs) from GCA patients was studied by immunohistochemistry and immunofluorescence. A clear monocytosis was seen in newly diagnosed GCA and PMR patients caused by elevated numbers of classical monocytes. Prednisone treatment suppressed numbers of non-classical monocytes. Both chemokine CX3CL1 and CCL2 were highly expressed in the TAB. Most macrophages in the TAB of GCA patients expressed non-classical monocyte markers CD16 and CX3CR1 whereas co-localisation of CD16 with classical monocyte marker CCR2 was infrequent. In conclusion, we report an altered distribution of monocyte subsets in both GCA and PMR patients. The majority of macrophages in TABs of GCA patients were CD68 + CD16 + CX3CR1 + CCR2− and thereby resembled the phenotype of non-classical monocytes.Yannick van SleenQi WangKornelis S. M. van der GeestJohanna WestraWayel H. AbdulahadPeter HeeringaAnnemieke M. H. BootsElisabeth BrouwerNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yannick van Sleen Qi Wang Kornelis S. M. van der Geest Johanna Westra Wayel H. Abdulahad Peter Heeringa Annemieke M. H. Boots Elisabeth Brouwer Involvement of Monocyte Subsets in the Immunopathology of Giant Cell Arteritis
description	Abstract Monocytes/macrophages are critical in systemic and local inflammation in giant cell arteritis (GCA) and possibly in clinically overlapping polymyalgia rheumatica (PMR). Therefore, we aimed to understand the contribution of monocyte subsets and the CX3CR1-CX3CL1 and CCR2-CCL2 migratory pathways, to the pathology of GCA. Peripheral blood monocytes were enumerated in samples from newly-diagnosed, untreated GCA and PMR patients and after prednisone-induced remission. The distribution of classical (CD14brightCD16neg) and the more pro-inflammatory, intermediate (CD14brightCD16+) and non-classical (CD14dimCD16+) monocyte subsets was analysed by flow cytometry. The phenotype of macrophages in temporal artery biopsies (TABs) from GCA patients was studied by immunohistochemistry and immunofluorescence. A clear monocytosis was seen in newly diagnosed GCA and PMR patients caused by elevated numbers of classical monocytes. Prednisone treatment suppressed numbers of non-classical monocytes. Both chemokine CX3CL1 and CCL2 were highly expressed in the TAB. Most macrophages in the TAB of GCA patients expressed non-classical monocyte markers CD16 and CX3CR1 whereas co-localisation of CD16 with classical monocyte marker CCR2 was infrequent. In conclusion, we report an altered distribution of monocyte subsets in both GCA and PMR patients. The majority of macrophages in TABs of GCA patients were CD68 + CD16 + CX3CR1 + CCR2− and thereby resembled the phenotype of non-classical monocytes.
format	article
author	Yannick van Sleen Qi Wang Kornelis S. M. van der Geest Johanna Westra Wayel H. Abdulahad Peter Heeringa Annemieke M. H. Boots Elisabeth Brouwer
author_facet	Yannick van Sleen Qi Wang Kornelis S. M. van der Geest Johanna Westra Wayel H. Abdulahad Peter Heeringa Annemieke M. H. Boots Elisabeth Brouwer
author_sort	Yannick van Sleen
title	Involvement of Monocyte Subsets in the Immunopathology of Giant Cell Arteritis
title_short	Involvement of Monocyte Subsets in the Immunopathology of Giant Cell Arteritis
title_full	Involvement of Monocyte Subsets in the Immunopathology of Giant Cell Arteritis
title_fullStr	Involvement of Monocyte Subsets in the Immunopathology of Giant Cell Arteritis
title_full_unstemmed	Involvement of Monocyte Subsets in the Immunopathology of Giant Cell Arteritis
title_sort	involvement of monocyte subsets in the immunopathology of giant cell arteritis
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/b86d4cd688644cae93d608708ac0a7e4
work_keys_str_mv	AT yannickvansleen involvementofmonocytesubsetsintheimmunopathologyofgiantcellarteritis AT qiwang involvementofmonocytesubsetsintheimmunopathologyofgiantcellarteritis AT kornelissmvandergeest involvementofmonocytesubsetsintheimmunopathologyofgiantcellarteritis AT johannawestra involvementofmonocytesubsetsintheimmunopathologyofgiantcellarteritis AT wayelhabdulahad involvementofmonocytesubsetsintheimmunopathologyofgiantcellarteritis AT peterheeringa involvementofmonocytesubsetsintheimmunopathologyofgiantcellarteritis AT annemiekemhboots involvementofmonocytesubsetsintheimmunopathologyofgiantcellarteritis AT elisabethbrouwer involvementofmonocytesubsetsintheimmunopathologyofgiantcellarteritis
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Involvement of Monocyte Subsets in the Immunopathology of Giant Cell Arteritis

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