Porous Bilayer Vascular Grafts Fabricated from Electrospinning of the Recombinant Human Collagen (RHC) Peptide-Based Blend

Cardiovascular diseases, including coronary artery and peripheral vascular pathologies, are leading causes of mortality. As an alternative to autografts, prosthetic grafts have been developed to reduce the death rate. This study presents the development and characterization of bilayer vascular graft...

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Autores principales:	Thi My Do, Yang Yang, Aipeng Deng
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	electrospinning recombinant human collagen (RHC) bilayer vascular grafts tissue engineering Organic chemistry QD241-441
Acceso en línea:	https://doaj.org/article/b86d5ccbfbba4ce59a7efd8d71123915
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spelling	oai:doaj.org-article:b86d5ccbfbba4ce59a7efd8d711239152021-11-25T18:49:38ZPorous Bilayer Vascular Grafts Fabricated from Electrospinning of the Recombinant Human Collagen (RHC) Peptide-Based Blend10.3390/polym132240422073-4360https://doaj.org/article/b86d5ccbfbba4ce59a7efd8d711239152021-11-01T00:00:00Zhttps://www.mdpi.com/2073-4360/13/22/4042https://doaj.org/toc/2073-4360Cardiovascular diseases, including coronary artery and peripheral vascular pathologies, are leading causes of mortality. As an alternative to autografts, prosthetic grafts have been developed to reduce the death rate. This study presents the development and characterization of bilayer vascular grafts with appropriate structural and biocompatibility properties. A polymer blend of recombinant human collagen (RHC) peptides and polycaprolactone (PCL) was used to build the inner layer of the graft by electrospinning and co-electrospinning the water-soluble polyethylene oxide (PEO) as sacrificial material together with PCL to generate the porous outer layer. The mechanical test demonstrated the bilayer scaffold’s appropriate mechanical properties as compared with the native vascular structure. Human umbilical vein endothelial cells (HUVEC) showed enhanced adhesion to the lumen after seeding on nanoscale fibers. Meanwhile, by enhancing the porosity of the microfibrous outer layer through the removal of PEO fibers, rat smooth muscle cells (A7r5) could proliferate and infiltrate the porous layer easily.Thi My DoYang YangAipeng DengMDPI AGarticleelectrospinningrecombinant human collagen (RHC)bilayer vascular graftstissue engineeringOrganic chemistryQD241-441ENPolymers, Vol 13, Iss 4042, p 4042 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	electrospinning recombinant human collagen (RHC) bilayer vascular grafts tissue engineering Organic chemistry QD241-441
spellingShingle	electrospinning recombinant human collagen (RHC) bilayer vascular grafts tissue engineering Organic chemistry QD241-441 Thi My Do Yang Yang Aipeng Deng Porous Bilayer Vascular Grafts Fabricated from Electrospinning of the Recombinant Human Collagen (RHC) Peptide-Based Blend
description	Cardiovascular diseases, including coronary artery and peripheral vascular pathologies, are leading causes of mortality. As an alternative to autografts, prosthetic grafts have been developed to reduce the death rate. This study presents the development and characterization of bilayer vascular grafts with appropriate structural and biocompatibility properties. A polymer blend of recombinant human collagen (RHC) peptides and polycaprolactone (PCL) was used to build the inner layer of the graft by electrospinning and co-electrospinning the water-soluble polyethylene oxide (PEO) as sacrificial material together with PCL to generate the porous outer layer. The mechanical test demonstrated the bilayer scaffold’s appropriate mechanical properties as compared with the native vascular structure. Human umbilical vein endothelial cells (HUVEC) showed enhanced adhesion to the lumen after seeding on nanoscale fibers. Meanwhile, by enhancing the porosity of the microfibrous outer layer through the removal of PEO fibers, rat smooth muscle cells (A7r5) could proliferate and infiltrate the porous layer easily.
format	article
author	Thi My Do Yang Yang Aipeng Deng
author_facet	Thi My Do Yang Yang Aipeng Deng
author_sort	Thi My Do
title	Porous Bilayer Vascular Grafts Fabricated from Electrospinning of the Recombinant Human Collagen (RHC) Peptide-Based Blend
title_short	Porous Bilayer Vascular Grafts Fabricated from Electrospinning of the Recombinant Human Collagen (RHC) Peptide-Based Blend
title_full	Porous Bilayer Vascular Grafts Fabricated from Electrospinning of the Recombinant Human Collagen (RHC) Peptide-Based Blend
title_fullStr	Porous Bilayer Vascular Grafts Fabricated from Electrospinning of the Recombinant Human Collagen (RHC) Peptide-Based Blend
title_full_unstemmed	Porous Bilayer Vascular Grafts Fabricated from Electrospinning of the Recombinant Human Collagen (RHC) Peptide-Based Blend
title_sort	porous bilayer vascular grafts fabricated from electrospinning of the recombinant human collagen (rhc) peptide-based blend
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/b86d5ccbfbba4ce59a7efd8d71123915
work_keys_str_mv	AT thimydo porousbilayervasculargraftsfabricatedfromelectrospinningoftherecombinanthumancollagenrhcpeptidebasedblend AT yangyang porousbilayervasculargraftsfabricatedfromelectrospinningoftherecombinanthumancollagenrhcpeptidebasedblend AT aipengdeng porousbilayervasculargraftsfabricatedfromelectrospinningoftherecombinanthumancollagenrhcpeptidebasedblend
_version_	1718410671479062528

Porous Bilayer Vascular Grafts Fabricated from Electrospinning of the Recombinant Human Collagen (RHC) Peptide-Based Blend

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