Defective membrane repair machinery impairs survival of invasive cancer cells

Abstract Cancer cells are able to reach distant tissues by migration and invasion processes. Enhanced ability to cope with physical stresses leading to cell membrane damages may offer to cancer cells high survival rate during metastasis. Consequently, down-regulation of the membrane repair machinery...

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Autores principales:	F. Bouvet, M. Ros, E. Bonedeau, C. Croissant, L. Frelin, F. Saltel, V. Moreau, A. Bouter
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b8704096970641338355e1028deb14e0
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spelling	oai:doaj.org-article:b8704096970641338355e1028deb14e02021-12-02T15:11:52ZDefective membrane repair machinery impairs survival of invasive cancer cells10.1038/s41598-020-77902-52045-2322https://doaj.org/article/b8704096970641338355e1028deb14e02020-12-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-77902-5https://doaj.org/toc/2045-2322Abstract Cancer cells are able to reach distant tissues by migration and invasion processes. Enhanced ability to cope with physical stresses leading to cell membrane damages may offer to cancer cells high survival rate during metastasis. Consequently, down-regulation of the membrane repair machinery may lead to metastasis inhibition. We show that migration of MDA-MB-231 cells on collagen I fibrils induces disruptions of plasma membrane and pullout of membrane fragments in the wake of cells. These cells are able to reseal membrane damages thanks to annexins (Anx) that are highly expressed in invasive cancer cells. In vitro membrane repair assays reveal that MDA-MB-231 cells respond heterogeneously to membrane injury and some of them possess a very efficient repair machinery. Finally, we show that silencing of AnxA5 and AnxA6 leads to the death of migrating MDA-MB-231 cells due to major defect of the membrane repair machinery. Disturbance of the membrane repair process may therefore provide a new avenue for inhibiting cancer metastasis.F. BouvetM. RosE. BonedeauC. CroissantL. FrelinF. SaltelV. MoreauA. BouterNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-16 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q F. Bouvet M. Ros E. Bonedeau C. Croissant L. Frelin F. Saltel V. Moreau A. Bouter Defective membrane repair machinery impairs survival of invasive cancer cells
description	Abstract Cancer cells are able to reach distant tissues by migration and invasion processes. Enhanced ability to cope with physical stresses leading to cell membrane damages may offer to cancer cells high survival rate during metastasis. Consequently, down-regulation of the membrane repair machinery may lead to metastasis inhibition. We show that migration of MDA-MB-231 cells on collagen I fibrils induces disruptions of plasma membrane and pullout of membrane fragments in the wake of cells. These cells are able to reseal membrane damages thanks to annexins (Anx) that are highly expressed in invasive cancer cells. In vitro membrane repair assays reveal that MDA-MB-231 cells respond heterogeneously to membrane injury and some of them possess a very efficient repair machinery. Finally, we show that silencing of AnxA5 and AnxA6 leads to the death of migrating MDA-MB-231 cells due to major defect of the membrane repair machinery. Disturbance of the membrane repair process may therefore provide a new avenue for inhibiting cancer metastasis.
format	article
author	F. Bouvet M. Ros E. Bonedeau C. Croissant L. Frelin F. Saltel V. Moreau A. Bouter
author_facet	F. Bouvet M. Ros E. Bonedeau C. Croissant L. Frelin F. Saltel V. Moreau A. Bouter
author_sort	F. Bouvet
title	Defective membrane repair machinery impairs survival of invasive cancer cells
title_short	Defective membrane repair machinery impairs survival of invasive cancer cells
title_full	Defective membrane repair machinery impairs survival of invasive cancer cells
title_fullStr	Defective membrane repair machinery impairs survival of invasive cancer cells
title_full_unstemmed	Defective membrane repair machinery impairs survival of invasive cancer cells
title_sort	defective membrane repair machinery impairs survival of invasive cancer cells
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/b8704096970641338355e1028deb14e0
work_keys_str_mv	AT fbouvet defectivemembranerepairmachineryimpairssurvivalofinvasivecancercells AT mros defectivemembranerepairmachineryimpairssurvivalofinvasivecancercells AT ebonedeau defectivemembranerepairmachineryimpairssurvivalofinvasivecancercells AT ccroissant defectivemembranerepairmachineryimpairssurvivalofinvasivecancercells AT lfrelin defectivemembranerepairmachineryimpairssurvivalofinvasivecancercells AT fsaltel defectivemembranerepairmachineryimpairssurvivalofinvasivecancercells AT vmoreau defectivemembranerepairmachineryimpairssurvivalofinvasivecancercells AT abouter defectivemembranerepairmachineryimpairssurvivalofinvasivecancercells
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Defective membrane repair machinery impairs survival of invasive cancer cells

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