Serological response in RT-PCR confirmed H1N1-2009 influenza a by hemagglutination inhibition and virus neutralization assays: an observational study.

<h4>Background</h4>We describe the serological response following H1N1-2009 influenza A infections confirmed by reverse-transcriptase polymerase chain reaction (RT-PCR).<h4>Methodology and principal findings</h4>The study included patients admitted to hospital, subjects of a...

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Autores principales:	Mark I Chen, Ian G Barr, Gerald C H Koh, Vernon J Lee, Caroline P S Lee, Robert Shaw, Cui Lin, Jonathan Yap, Alex R Cook, Boon Huan Tan, Jin Phang Loh, Timothy Barkham, Vincent T K Chow, Raymond T P Lin, Yee-Sin Leo
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2010
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b871e047807244afb607b4dfaa45a11c
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spelling	oai:doaj.org-article:b871e047807244afb607b4dfaa45a11c2021-11-18T06:35:36ZSerological response in RT-PCR confirmed H1N1-2009 influenza a by hemagglutination inhibition and virus neutralization assays: an observational study.1932-620310.1371/journal.pone.0012474https://doaj.org/article/b871e047807244afb607b4dfaa45a11c2010-08-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/20814575/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>We describe the serological response following H1N1-2009 influenza A infections confirmed by reverse-transcriptase polymerase chain reaction (RT-PCR).<h4>Methodology and principal findings</h4>The study included patients admitted to hospital, subjects of a seroepidemiologic cohort study, and participants identified from outbreak studies in Singapore. Baseline (first available blood sample) and follow-up blood samples were analyzed for antibody titers to H1N1-2009 and recently circulating seasonal influenza A virus strains by hemagglutination inhibition (HI) and virus micro-neutralization (VM) assays. 267 samples from 118 cases of H1N1-2009 were analyzed. Geometric mean titers by HI peaked at 123 (95% confidence interval, CI 43-356) between days 30 to 39. The chance of observing seroconversion (four-fold or greater increase of antibodies) was maximized when restricting analysis to 45 participants with baseline sera collected within 5 days of onset and follow-up sera collected 15 or more days after onset; for these participants, 82% and 89% seroconverted to A/California/7/2009 H1N1 by HI and VM respectively. A four-fold or greater increase in cross-reactive antibody titers to seasonal A/Brisbane/59/2007 H1N1, A/Brisbane/10/2007 H3N2 and A/Wisconsin/15/2009 H3N2 occurred in 20%, 18% and 16% of participants respectively.<h4>Conclusions and significance</h4>Appropriately timed paired serology detects 80-90% RT-PCR confirmed H1N1-2009; Antibodies from infection with H1N1-2009 cross-reacted with seasonal influenza viruses.Mark I ChenIan G BarrGerald C H KohVernon J LeeCaroline P S LeeRobert ShawCui LinJonathan YapAlex R CookBoon Huan TanJin Phang LohTimothy BarkhamVincent T K ChowRaymond T P LinYee-Sin LeoPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 5, Iss 8, p e12474 (2010)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Mark I Chen Ian G Barr Gerald C H Koh Vernon J Lee Caroline P S Lee Robert Shaw Cui Lin Jonathan Yap Alex R Cook Boon Huan Tan Jin Phang Loh Timothy Barkham Vincent T K Chow Raymond T P Lin Yee-Sin Leo Serological response in RT-PCR confirmed H1N1-2009 influenza a by hemagglutination inhibition and virus neutralization assays: an observational study.
description	<h4>Background</h4>We describe the serological response following H1N1-2009 influenza A infections confirmed by reverse-transcriptase polymerase chain reaction (RT-PCR).<h4>Methodology and principal findings</h4>The study included patients admitted to hospital, subjects of a seroepidemiologic cohort study, and participants identified from outbreak studies in Singapore. Baseline (first available blood sample) and follow-up blood samples were analyzed for antibody titers to H1N1-2009 and recently circulating seasonal influenza A virus strains by hemagglutination inhibition (HI) and virus micro-neutralization (VM) assays. 267 samples from 118 cases of H1N1-2009 were analyzed. Geometric mean titers by HI peaked at 123 (95% confidence interval, CI 43-356) between days 30 to 39. The chance of observing seroconversion (four-fold or greater increase of antibodies) was maximized when restricting analysis to 45 participants with baseline sera collected within 5 days of onset and follow-up sera collected 15 or more days after onset; for these participants, 82% and 89% seroconverted to A/California/7/2009 H1N1 by HI and VM respectively. A four-fold or greater increase in cross-reactive antibody titers to seasonal A/Brisbane/59/2007 H1N1, A/Brisbane/10/2007 H3N2 and A/Wisconsin/15/2009 H3N2 occurred in 20%, 18% and 16% of participants respectively.<h4>Conclusions and significance</h4>Appropriately timed paired serology detects 80-90% RT-PCR confirmed H1N1-2009; Antibodies from infection with H1N1-2009 cross-reacted with seasonal influenza viruses.
format	article
author	Mark I Chen Ian G Barr Gerald C H Koh Vernon J Lee Caroline P S Lee Robert Shaw Cui Lin Jonathan Yap Alex R Cook Boon Huan Tan Jin Phang Loh Timothy Barkham Vincent T K Chow Raymond T P Lin Yee-Sin Leo
author_facet	Mark I Chen Ian G Barr Gerald C H Koh Vernon J Lee Caroline P S Lee Robert Shaw Cui Lin Jonathan Yap Alex R Cook Boon Huan Tan Jin Phang Loh Timothy Barkham Vincent T K Chow Raymond T P Lin Yee-Sin Leo
author_sort	Mark I Chen
title	Serological response in RT-PCR confirmed H1N1-2009 influenza a by hemagglutination inhibition and virus neutralization assays: an observational study.
title_short	Serological response in RT-PCR confirmed H1N1-2009 influenza a by hemagglutination inhibition and virus neutralization assays: an observational study.
title_full	Serological response in RT-PCR confirmed H1N1-2009 influenza a by hemagglutination inhibition and virus neutralization assays: an observational study.
title_fullStr	Serological response in RT-PCR confirmed H1N1-2009 influenza a by hemagglutination inhibition and virus neutralization assays: an observational study.
title_full_unstemmed	Serological response in RT-PCR confirmed H1N1-2009 influenza a by hemagglutination inhibition and virus neutralization assays: an observational study.
title_sort	serological response in rt-pcr confirmed h1n1-2009 influenza a by hemagglutination inhibition and virus neutralization assays: an observational study.
publisher	Public Library of Science (PLoS)
publishDate	2010
url	https://doaj.org/article/b871e047807244afb607b4dfaa45a11c
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Serological response in RT-PCR confirmed H1N1-2009 influenza a by hemagglutination inhibition and virus neutralization assays: an observational study.

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