Effect of Valeriana Sysimberifolia Extract on VEGF Expression in A549 Cell Line

BACKGROUND AND OBJECTIVE: Today, the use of medicinal plants in the treatment of cancer is more important due to its fewer side effects. Vascular endothelial growth factor (VEGF) is one of the most important factors in stimulating angiogenesis and promoting cancer. The aim of this study was to inves...

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Autores principales:	S Chegini, F Tafvizi, H Noorbazargan
Formato:	article
Lenguaje:	EN FA
Publicado:	Babol University of Medical Sciences 2020
Materias:	valeriana sysimberifolia vegf lung cancer. Medicine R Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/b873c40beb984b9e9ced83af51949943
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spelling	oai:doaj.org-article:b873c40beb984b9e9ced83af519499432021-11-09T10:15:54ZEffect of Valeriana Sysimberifolia Extract on VEGF Expression in A549 Cell Line1561-41072251-7170https://doaj.org/article/b873c40beb984b9e9ced83af519499432020-03-01T00:00:00Zhttp://jbums.org/article-1-8477-en.htmlhttps://doaj.org/toc/1561-4107https://doaj.org/toc/2251-7170BACKGROUND AND OBJECTIVE: Today, the use of medicinal plants in the treatment of cancer is more important due to its fewer side effects. Vascular endothelial growth factor (VEGF) is one of the most important factors in stimulating angiogenesis and promoting cancer. The aim of this study was to investigate the inhibitory effect of Valeriana Sysimberifolia root extract on proliferation of lung cancer cells (A549) and VEGF gene expression in treated cells. METHODS: The hydro alcoholic extract of Valeriana Sysimberifolia root was prepared by percolation. After preparation of cell line A549 from the Iranian Biological Resource Center, cancer cells were treated in culture medium with different concentrations of plant root extract (50, 25, 12.5, 6.25, 3.12, 1.56, 0.78 µg/ml) at 24, 48 and 72 hours and viability was performed by MTT assay at all three time. Real Time-PCR method was used to evaluate the expression of VEGF gene in cancer cells treated with IC50 concentration of plant extract. FINDINGS: With increasing the concentration of the extract and the treatment time, a decrease in viability of cancer cells was observed. The highest inhibition of cell proliferation was observed at a concentration of 50µg/ml after 72 hours (94% growth inhibition) which was significant compared to the control group (p= 0.0001). Also, with increasing treatment time, decreased VEGF gene expression was seen compared to the control group. Thus, a two times decrease in VEGF gene expression was seen in cells after 72 hours (p= 0.0017). CONCLUSION: Valeriana Sysimberifolia root extract has anti-proliferative and anti-angiogenesis activity. It seems that further studies can be used as a biological product in the treatment of cancer.S CheginiF TafviziH NoorbazarganBabol University of Medical Sciencesarticlevaleriana sysimberifoliavegflung cancer.MedicineRMedicine (General)R5-920ENFAMajallah-i Dānishgāh-i ̒Ulūm-i Pizishkī-i Bābul, Vol 22, Iss 1, Pp 222-228 (2020)
institution	DOAJ
collection	DOAJ
language	EN FA
topic	valeriana sysimberifolia vegf lung cancer. Medicine R Medicine (General) R5-920
spellingShingle	valeriana sysimberifolia vegf lung cancer. Medicine R Medicine (General) R5-920 S Chegini F Tafvizi H Noorbazargan Effect of Valeriana Sysimberifolia Extract on VEGF Expression in A549 Cell Line
description	BACKGROUND AND OBJECTIVE: Today, the use of medicinal plants in the treatment of cancer is more important due to its fewer side effects. Vascular endothelial growth factor (VEGF) is one of the most important factors in stimulating angiogenesis and promoting cancer. The aim of this study was to investigate the inhibitory effect of Valeriana Sysimberifolia root extract on proliferation of lung cancer cells (A549) and VEGF gene expression in treated cells. METHODS: The hydro alcoholic extract of Valeriana Sysimberifolia root was prepared by percolation. After preparation of cell line A549 from the Iranian Biological Resource Center, cancer cells were treated in culture medium with different concentrations of plant root extract (50, 25, 12.5, 6.25, 3.12, 1.56, 0.78 µg/ml) at 24, 48 and 72 hours and viability was performed by MTT assay at all three time. Real Time-PCR method was used to evaluate the expression of VEGF gene in cancer cells treated with IC50 concentration of plant extract. FINDINGS: With increasing the concentration of the extract and the treatment time, a decrease in viability of cancer cells was observed. The highest inhibition of cell proliferation was observed at a concentration of 50µg/ml after 72 hours (94% growth inhibition) which was significant compared to the control group (p= 0.0001). Also, with increasing treatment time, decreased VEGF gene expression was seen compared to the control group. Thus, a two times decrease in VEGF gene expression was seen in cells after 72 hours (p= 0.0017). CONCLUSION: Valeriana Sysimberifolia root extract has anti-proliferative and anti-angiogenesis activity. It seems that further studies can be used as a biological product in the treatment of cancer.
format	article
author	S Chegini F Tafvizi H Noorbazargan
author_facet	S Chegini F Tafvizi H Noorbazargan
author_sort	S Chegini
title	Effect of Valeriana Sysimberifolia Extract on VEGF Expression in A549 Cell Line
title_short	Effect of Valeriana Sysimberifolia Extract on VEGF Expression in A549 Cell Line
title_full	Effect of Valeriana Sysimberifolia Extract on VEGF Expression in A549 Cell Line
title_fullStr	Effect of Valeriana Sysimberifolia Extract on VEGF Expression in A549 Cell Line
title_full_unstemmed	Effect of Valeriana Sysimberifolia Extract on VEGF Expression in A549 Cell Line
title_sort	effect of valeriana sysimberifolia extract on vegf expression in a549 cell line
publisher	Babol University of Medical Sciences
publishDate	2020
url	https://doaj.org/article/b873c40beb984b9e9ced83af51949943
work_keys_str_mv	AT schegini effectofvalerianasysimberifoliaextractonvegfexpressionina549cellline AT ftafvizi effectofvalerianasysimberifoliaextractonvegfexpressionina549cellline AT hnoorbazargan effectofvalerianasysimberifoliaextractonvegfexpressionina549cellline
_version_	1718441141355937792

Effect of Valeriana Sysimberifolia Extract on VEGF Expression in A549 Cell Line

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