Genetically Encoded Fluorescent Biosensors for Biomedical Applications

One of the challenges of modern biology and medicine is to visualize biomolecules in their natural environment, in real-time and in a non-invasive fashion, so as to gain insight into their physiological behavior and highlight alterations in pathological settings, which will enable to devise appropri...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Vera S. Ovechkina, Suren M. Zakian, Sergey P. Medvedev, Kamila R. Valetdinova
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	genetically encoded fluorescent biosensors fluorescent protein drug screening Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/b8756358184d4301ba9598b659348978
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:b8756358184d4301ba9598b659348978
record_format	dspace
spelling	oai:doaj.org-article:b8756358184d4301ba9598b6593489782021-11-25T16:48:36ZGenetically Encoded Fluorescent Biosensors for Biomedical Applications10.3390/biomedicines91115282227-9059https://doaj.org/article/b8756358184d4301ba9598b6593489782021-10-01T00:00:00Zhttps://www.mdpi.com/2227-9059/9/11/1528https://doaj.org/toc/2227-9059One of the challenges of modern biology and medicine is to visualize biomolecules in their natural environment, in real-time and in a non-invasive fashion, so as to gain insight into their physiological behavior and highlight alterations in pathological settings, which will enable to devise appropriate therapeutic strategies. Genetically encoded fluorescent biosensors constitute a class of imaging agents that enable visualization of biological processes and events directly in situ, preserving the native biological context and providing detailed insight into their localization and dynamics in cells. Real-time monitoring of drug action in a specific cellular compartment, organ, or tissue type; the ability to screen at the single-cell resolution; and the elimination of false-positive results caused by low drug bioavailability that is not detected by in vitro testing methods are a few of the obvious benefits of using genetically encoded fluorescent biosensors in drug screening. This review summarizes results of the studies that have been conducted in the last years toward the fabrication of genetically encoded fluorescent biosensors for biomedical applications with a comprehensive discussion on the challenges, future trends, and potential inputs needed for improving them.Vera S. OvechkinaSuren M. ZakianSergey P. MedvedevKamila R. ValetdinovaMDPI AGarticlegenetically encoded fluorescent biosensorsfluorescent proteindrug screeningBiology (General)QH301-705.5ENBiomedicines, Vol 9, Iss 1528, p 1528 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	genetically encoded fluorescent biosensors fluorescent protein drug screening Biology (General) QH301-705.5
spellingShingle	genetically encoded fluorescent biosensors fluorescent protein drug screening Biology (General) QH301-705.5 Vera S. Ovechkina Suren M. Zakian Sergey P. Medvedev Kamila R. Valetdinova Genetically Encoded Fluorescent Biosensors for Biomedical Applications
description	One of the challenges of modern biology and medicine is to visualize biomolecules in their natural environment, in real-time and in a non-invasive fashion, so as to gain insight into their physiological behavior and highlight alterations in pathological settings, which will enable to devise appropriate therapeutic strategies. Genetically encoded fluorescent biosensors constitute a class of imaging agents that enable visualization of biological processes and events directly in situ, preserving the native biological context and providing detailed insight into their localization and dynamics in cells. Real-time monitoring of drug action in a specific cellular compartment, organ, or tissue type; the ability to screen at the single-cell resolution; and the elimination of false-positive results caused by low drug bioavailability that is not detected by in vitro testing methods are a few of the obvious benefits of using genetically encoded fluorescent biosensors in drug screening. This review summarizes results of the studies that have been conducted in the last years toward the fabrication of genetically encoded fluorescent biosensors for biomedical applications with a comprehensive discussion on the challenges, future trends, and potential inputs needed for improving them.
format	article
author	Vera S. Ovechkina Suren M. Zakian Sergey P. Medvedev Kamila R. Valetdinova
author_facet	Vera S. Ovechkina Suren M. Zakian Sergey P. Medvedev Kamila R. Valetdinova
author_sort	Vera S. Ovechkina
title	Genetically Encoded Fluorescent Biosensors for Biomedical Applications
title_short	Genetically Encoded Fluorescent Biosensors for Biomedical Applications
title_full	Genetically Encoded Fluorescent Biosensors for Biomedical Applications
title_fullStr	Genetically Encoded Fluorescent Biosensors for Biomedical Applications
title_full_unstemmed	Genetically Encoded Fluorescent Biosensors for Biomedical Applications
title_sort	genetically encoded fluorescent biosensors for biomedical applications
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/b8756358184d4301ba9598b659348978
work_keys_str_mv	AT verasovechkina geneticallyencodedfluorescentbiosensorsforbiomedicalapplications AT surenmzakian geneticallyencodedfluorescentbiosensorsforbiomedicalapplications AT sergeypmedvedev geneticallyencodedfluorescentbiosensorsforbiomedicalapplications AT kamilarvaletdinova geneticallyencodedfluorescentbiosensorsforbiomedicalapplications
_version_	1718412978347311104

Genetically Encoded Fluorescent Biosensors for Biomedical Applications

Ejemplares similares