Global Spread and Molecular Characterization of CTX-M-Producing <i>Salmonella</i> Typhimurium Isolates

Global Spread and Molecular Characterization of CTX-M-Producing <i>Salmonella</i> Typhimurium Isolates

This study aimed to determine the global prevalence and molecular characterization of CTX-M-producing <i>Salmonella</i> Typhimurium isolates. A total of 330 (15.2%, 330/21779) <i>bla</i><sub>CTX-M</sub>-positive <i>S</i>. Typhimurium were obtained from...

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Autores principales: Lili Guo, Yongda Zhao
Formato: article
Lenguaje:EN
Publicado: MDPI AG 2021
Materias:
CTX-M
<i>Salmonella</i> Typhimurium
global distribution
MLST
ARGs
Therapeutics. Pharmacology
RM1-950
Acceso en línea:https://doaj.org/article/b881f13efb40442d838318716a762e67
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spelling oai:doaj.org-article:b881f13efb40442d838318716a762e672021-11-25T16:25:04ZGlobal Spread and Molecular Characterization of CTX-M-Producing <i>Salmonella</i> Typhimurium Isolates10.3390/antibiotics101114172079-6382https://doaj.org/article/b881f13efb40442d838318716a762e672021-11-01T00:00:00Zhttps://www.mdpi.com/2079-6382/10/11/1417https://doaj.org/toc/2079-6382This study aimed to determine the global prevalence and molecular characterization of CTX-M-producing <i>Salmonella</i> Typhimurium isolates. A total of 330 (15.2%, 330/21779) <i>bla</i><sub>CTX-M</sub>-positive <i>S</i>. Typhimurium were obtained from the public databases in July 2021. Thirteen variants were found in the 330 members of the <i>bla</i><sub>CTX-M</sub> group, and <i>bla</i><sub>CTX-M-9</sub> (26.4%, 88/330) was the most prevalent. The majority of <i>bla</i><sub>CTX-M</sub>-positive <i>S.</i> Typhimurium were obtained from humans (59.7%, 197/330) and animals (31.5%, 104/330). The number of <i>bla</i><sub>CTX-M</sub>-positive <i>S.</i> Typhimurium increased annually (<i>p</i> < 0.0001). These isolates were primarily found from China, the United Kingdom, Australia, the USA, and Germany. In addition, these isolates possessed 14 distinct sequence types (ST), and three predominated: ST34 (42.7%, 141/330), ST19 (37.0%, 122/330), and ST313 (10.3%, 34/330). The majority of ST34 <i>S.</i> Typhimurium isolates were distributed in China and mainly from swine. However, the majority of ST19 were distributed in the United Kingdom and Australia. Analysis of contigs showed that the major type of <i>bla</i><sub>CTX-M</sub>-carrying plasmid was identified as IncI plasmid (52.9%, 27/51) and IncHI2 plasmid (17.6%, 9/51) in 51 <i>bla</i><sub>CTX-M</sub>-positive S. Typhimurium isolates. In addition, WGS analysis further revealed that <i>bla</i><sub>CTX-M</sub> co-existed with nine antibiotic-resistant genes with a detection rate over 50%, conferring resistance to five classes of antimicrobials. The 154 virulence genes were detected among these isolates, of which 107 virulence genes were highly common. This study revealed that China has been severely contaminated by <i>bla</i><sub>CTX-M</sub>-positive <i>S.</i> Typhimurium isolates, these isolates possessed numerous ARGs and virulence genes, and highlighted that continued vigilance for <i>bla</i><sub>CTX-M</sub>-positive <i>S.</i> Typhimurium in animals and humans is urgently needed.Lili GuoYongda ZhaoMDPI AGarticleCTX-M<i>Salmonella</i> Typhimuriumglobal distributionMLSTARGsTherapeutics. PharmacologyRM1-950ENAntibiotics, Vol 10, Iss 1417, p 1417 (2021)
institution DOAJ
collection DOAJ
language EN
topic CTX-M
<i>Salmonella</i> Typhimurium
global distribution
MLST
ARGs
Therapeutics. Pharmacology
RM1-950
spellingShingle CTX-M
<i>Salmonella</i> Typhimurium
global distribution
MLST
ARGs
Therapeutics. Pharmacology
RM1-950
Lili Guo
Yongda Zhao
Global Spread and Molecular Characterization of CTX-M-Producing <i>Salmonella</i> Typhimurium Isolates
description This study aimed to determine the global prevalence and molecular characterization of CTX-M-producing <i>Salmonella</i> Typhimurium isolates. A total of 330 (15.2%, 330/21779) <i>bla</i><sub>CTX-M</sub>-positive <i>S</i>. Typhimurium were obtained from the public databases in July 2021. Thirteen variants were found in the 330 members of the <i>bla</i><sub>CTX-M</sub> group, and <i>bla</i><sub>CTX-M-9</sub> (26.4%, 88/330) was the most prevalent. The majority of <i>bla</i><sub>CTX-M</sub>-positive <i>S.</i> Typhimurium were obtained from humans (59.7%, 197/330) and animals (31.5%, 104/330). The number of <i>bla</i><sub>CTX-M</sub>-positive <i>S.</i> Typhimurium increased annually (<i>p</i> < 0.0001). These isolates were primarily found from China, the United Kingdom, Australia, the USA, and Germany. In addition, these isolates possessed 14 distinct sequence types (ST), and three predominated: ST34 (42.7%, 141/330), ST19 (37.0%, 122/330), and ST313 (10.3%, 34/330). The majority of ST34 <i>S.</i> Typhimurium isolates were distributed in China and mainly from swine. However, the majority of ST19 were distributed in the United Kingdom and Australia. Analysis of contigs showed that the major type of <i>bla</i><sub>CTX-M</sub>-carrying plasmid was identified as IncI plasmid (52.9%, 27/51) and IncHI2 plasmid (17.6%, 9/51) in 51 <i>bla</i><sub>CTX-M</sub>-positive S. Typhimurium isolates. In addition, WGS analysis further revealed that <i>bla</i><sub>CTX-M</sub> co-existed with nine antibiotic-resistant genes with a detection rate over 50%, conferring resistance to five classes of antimicrobials. The 154 virulence genes were detected among these isolates, of which 107 virulence genes were highly common. This study revealed that China has been severely contaminated by <i>bla</i><sub>CTX-M</sub>-positive <i>S.</i> Typhimurium isolates, these isolates possessed numerous ARGs and virulence genes, and highlighted that continued vigilance for <i>bla</i><sub>CTX-M</sub>-positive <i>S.</i> Typhimurium in animals and humans is urgently needed.
format article
author Lili Guo
Yongda Zhao
author_facet Lili Guo
Yongda Zhao
author_sort Lili Guo
title Global Spread and Molecular Characterization of CTX-M-Producing <i>Salmonella</i> Typhimurium Isolates
title_short Global Spread and Molecular Characterization of CTX-M-Producing <i>Salmonella</i> Typhimurium Isolates
title_full Global Spread and Molecular Characterization of CTX-M-Producing <i>Salmonella</i> Typhimurium Isolates
title_fullStr Global Spread and Molecular Characterization of CTX-M-Producing <i>Salmonella</i> Typhimurium Isolates
title_full_unstemmed Global Spread and Molecular Characterization of CTX-M-Producing <i>Salmonella</i> Typhimurium Isolates
title_sort global spread and molecular characterization of ctx-m-producing <i>salmonella</i> typhimurium isolates
publisher MDPI AG
publishDate 2021
url https://doaj.org/article/b881f13efb40442d838318716a762e67
work_keys_str_mv AT liliguo globalspreadandmolecularcharacterizationofctxmproducingisalmonellaityphimuriumisolates
AT yongdazhao globalspreadandmolecularcharacterizationofctxmproducingisalmonellaityphimuriumisolates
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