Src-1 and SP2 promote the proliferation and epithelial–mesenchymal transition of nasopharyngeal carcinoma

Nasopharyngeal carcinoma (NPC) is characterized by high morbidity and morality, especially in Southern China. Transcription factors intensively participate in the initiation and development of NPC. This study aimed to investigate the roles of Src-1 in NPC. mRNA level was determined by qRT-PCR. Weste...

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Autores principales:	Zhang Jingjing, Yang Yuanyuan, Liu Hongyu, Hu Hongyi
Formato:	article
Lenguaje:	EN
Publicado:	De Gruyter 2021
Materias:	nasopharyngeal carcinoma steroid receptor coactivator 1 sp2 epithelial–mesenchymal transition Medicine R
Acceso en línea:	https://doaj.org/article/b8858f802eaf41f1a90af2c80f6eb0b6
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spelling	oai:doaj.org-article:b8858f802eaf41f1a90af2c80f6eb0b62021-12-05T14:10:54ZSrc-1 and SP2 promote the proliferation and epithelial–mesenchymal transition of nasopharyngeal carcinoma2391-546310.1515/med-2021-0248https://doaj.org/article/b8858f802eaf41f1a90af2c80f6eb0b62021-07-01T00:00:00Zhttps://doi.org/10.1515/med-2021-0248https://doaj.org/toc/2391-5463Nasopharyngeal carcinoma (NPC) is characterized by high morbidity and morality, especially in Southern China. Transcription factors intensively participate in the initiation and development of NPC. This study aimed to investigate the roles of Src-1 in NPC. mRNA level was determined by qRT-PCR. Western blot was carried out for the protein level. CCK-8 assay was performed to determine cell viability, colony formation for NPC cell proliferation, and transwell for cell migration and invasion ability. The results showed Steroid receptor coactivator 1 (Src-1) was overexpressed in SNE-2 and 6-10B. The expression of Src-1 and SP2 was in positive correlation. Overexpression of Src-1 promoted the cell viability, colony formation, and epithelial–mesenchymal transition (EMT), manifested by the increase of migration and invasion ability, while knockdown of Src-1 exerted opposite effects. Additionally, knockdown or overexpression of SP2 reversed the effects of overexpressed or downregulated Src-1, which was reversed by the depletion of SP2. Moreover, Src-1 interacted with SP2 to regulate EMT-related genes such as E-cad, N-cad, Vimentin, and ZEB1, and proliferation- and apoptosis-related genes, such as bax, cytochrome c, and cleaved caspase3 and bcl-2. Thus, blocking the interaction between Src-1 and SP2 may be a therapeutic target for inhibiting the metastasis of NPC.Zhang JingjingYang YuanyuanLiu HongyuHu HongyiDe Gruyterarticlenasopharyngeal carcinomasteroid receptor coactivator 1sp2epithelial–mesenchymal transitionMedicineRENOpen Medicine, Vol 16, Iss 1, Pp 1061-1069 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	nasopharyngeal carcinoma steroid receptor coactivator 1 sp2 epithelial–mesenchymal transition Medicine R
spellingShingle	nasopharyngeal carcinoma steroid receptor coactivator 1 sp2 epithelial–mesenchymal transition Medicine R Zhang Jingjing Yang Yuanyuan Liu Hongyu Hu Hongyi Src-1 and SP2 promote the proliferation and epithelial–mesenchymal transition of nasopharyngeal carcinoma
description	Nasopharyngeal carcinoma (NPC) is characterized by high morbidity and morality, especially in Southern China. Transcription factors intensively participate in the initiation and development of NPC. This study aimed to investigate the roles of Src-1 in NPC. mRNA level was determined by qRT-PCR. Western blot was carried out for the protein level. CCK-8 assay was performed to determine cell viability, colony formation for NPC cell proliferation, and transwell for cell migration and invasion ability. The results showed Steroid receptor coactivator 1 (Src-1) was overexpressed in SNE-2 and 6-10B. The expression of Src-1 and SP2 was in positive correlation. Overexpression of Src-1 promoted the cell viability, colony formation, and epithelial–mesenchymal transition (EMT), manifested by the increase of migration and invasion ability, while knockdown of Src-1 exerted opposite effects. Additionally, knockdown or overexpression of SP2 reversed the effects of overexpressed or downregulated Src-1, which was reversed by the depletion of SP2. Moreover, Src-1 interacted with SP2 to regulate EMT-related genes such as E-cad, N-cad, Vimentin, and ZEB1, and proliferation- and apoptosis-related genes, such as bax, cytochrome c, and cleaved caspase3 and bcl-2. Thus, blocking the interaction between Src-1 and SP2 may be a therapeutic target for inhibiting the metastasis of NPC.
format	article
author	Zhang Jingjing Yang Yuanyuan Liu Hongyu Hu Hongyi
author_facet	Zhang Jingjing Yang Yuanyuan Liu Hongyu Hu Hongyi
author_sort	Zhang Jingjing
title	Src-1 and SP2 promote the proliferation and epithelial–mesenchymal transition of nasopharyngeal carcinoma
title_short	Src-1 and SP2 promote the proliferation and epithelial–mesenchymal transition of nasopharyngeal carcinoma
title_full	Src-1 and SP2 promote the proliferation and epithelial–mesenchymal transition of nasopharyngeal carcinoma
title_fullStr	Src-1 and SP2 promote the proliferation and epithelial–mesenchymal transition of nasopharyngeal carcinoma
title_full_unstemmed	Src-1 and SP2 promote the proliferation and epithelial–mesenchymal transition of nasopharyngeal carcinoma
title_sort	src-1 and sp2 promote the proliferation and epithelial–mesenchymal transition of nasopharyngeal carcinoma
publisher	De Gruyter
publishDate	2021
url	https://doaj.org/article/b8858f802eaf41f1a90af2c80f6eb0b6
work_keys_str_mv	AT zhangjingjing src1andsp2promotetheproliferationandepithelialmesenchymaltransitionofnasopharyngealcarcinoma AT yangyuanyuan src1andsp2promotetheproliferationandepithelialmesenchymaltransitionofnasopharyngealcarcinoma AT liuhongyu src1andsp2promotetheproliferationandepithelialmesenchymaltransitionofnasopharyngealcarcinoma AT huhongyi src1andsp2promotetheproliferationandepithelialmesenchymaltransitionofnasopharyngealcarcinoma
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Src-1 and SP2 promote the proliferation and epithelial–mesenchymal transition of nasopharyngeal carcinoma

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