Study of the mechanism of environmentally friendly translucent balsa-modified lysozyme dressing for facilitating wound healing

Daijun Zhou, Tao Yang, Wei Qian, Malcolm Xing, Gaoxing Luo Institute of Burn Research; State Key Laboratory of Trauma, Burn and Combined Injury; Southwest Hospital, Third Military Medical University (Army Medical University), Chongqing, China Objective: This study aimed to prepare an eco-friendly...

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Autores principales:	Zhou D, Yang T, Qian W, Xing M, Luo G
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2018
Materias:	Balsa Lysozyme Wound dressing Wound healing Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/b8894bf285b94ec09c9361c653088083
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spelling	oai:doaj.org-article:b8894bf285b94ec09c9361c6530880832021-12-02T03:01:47ZStudy of the mechanism of environmentally friendly translucent balsa-modified lysozyme dressing for facilitating wound healing1178-2013https://doaj.org/article/b8894bf285b94ec09c9361c6530880832018-07-01T00:00:00Zhttps://www.dovepress.com/study-of-the-mechanism-of-environmentally-friendly-translucent-balsa-m-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Daijun Zhou, Tao Yang, Wei Qian, Malcolm Xing, Gaoxing Luo Institute of Burn Research; State Key Laboratory of Trauma, Burn and Combined Injury; Southwest Hospital, Third Military Medical University (Army Medical University), Chongqing, China Objective: This study aimed to prepare an eco-friendly dressing using a balsa-derived membrane with lysozymes designed for antibacterial purposes.Methods: The groups included controls, balsa (group A), translucent balsa (group B), translucent balsa–lysozymes (group C), and translucent balsa-modified lysozymes (group D). Physical and chemical methods were used to characterize the materials, and the function of the materials was evaluated by in vivo and in vitro experiments.Results: Antibacterial activity against Escherichia coli and Staphylococcus aureus was ordered D > C > B ≈ A (P<0.05). Healing rates in the control, A, B, C, and D groups were 30.6%, 48.3%, 56.7%, 70.9%, and 79.2%, respectively at 7 days after injury. The lengths of new epithelia of the wound surface were ordered D > C > B ≈ A > control (P<0.05). Reverse-transcription polymerase chain reaction showed that expression of Wnt3a, β-catenin, and PCNA mRNA were ordered D > C > B ≈ A > control (P<0.05). The order of expression of PCNA was D > C > B ≈ A > control (P<0.05). There were no differences in GSK3β expression (P>0.05). The order of expression of axin was D < C < B ≈ A < control (P<0.05). The cell-migration rate at 24 hours was ordered D > C > B ≈ A > control (P<0.05).Conclusion: This translucent balsa-modified lysozyme dressing is characterized by strong antibacterial properties, stable and persistent release, no cytotoxicity, and capacity to promote antibacterial ability and epithelial growth, as well as cell proliferation and migration.Keywords: balsa, lysozyme, wound dressing, wound healingZhou DYang TQian WXing MLuo GDove Medical PressarticleBalsaLysozymeWound dressingWound healingMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 13, Pp 4171-4187 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Balsa Lysozyme Wound dressing Wound healing Medicine (General) R5-920
spellingShingle	Balsa Lysozyme Wound dressing Wound healing Medicine (General) R5-920 Zhou D Yang T Qian W Xing M Luo G Study of the mechanism of environmentally friendly translucent balsa-modified lysozyme dressing for facilitating wound healing
description	Daijun Zhou, Tao Yang, Wei Qian, Malcolm Xing, Gaoxing Luo Institute of Burn Research; State Key Laboratory of Trauma, Burn and Combined Injury; Southwest Hospital, Third Military Medical University (Army Medical University), Chongqing, China Objective: This study aimed to prepare an eco-friendly dressing using a balsa-derived membrane with lysozymes designed for antibacterial purposes.Methods: The groups included controls, balsa (group A), translucent balsa (group B), translucent balsa–lysozymes (group C), and translucent balsa-modified lysozymes (group D). Physical and chemical methods were used to characterize the materials, and the function of the materials was evaluated by in vivo and in vitro experiments.Results: Antibacterial activity against Escherichia coli and Staphylococcus aureus was ordered D > C > B ≈ A (P<0.05). Healing rates in the control, A, B, C, and D groups were 30.6%, 48.3%, 56.7%, 70.9%, and 79.2%, respectively at 7 days after injury. The lengths of new epithelia of the wound surface were ordered D > C > B ≈ A > control (P<0.05). Reverse-transcription polymerase chain reaction showed that expression of Wnt3a, β-catenin, and PCNA mRNA were ordered D > C > B ≈ A > control (P<0.05). The order of expression of PCNA was D > C > B ≈ A > control (P<0.05). There were no differences in GSK3β expression (P>0.05). The order of expression of axin was D < C < B ≈ A < control (P<0.05). The cell-migration rate at 24 hours was ordered D > C > B ≈ A > control (P<0.05).Conclusion: This translucent balsa-modified lysozyme dressing is characterized by strong antibacterial properties, stable and persistent release, no cytotoxicity, and capacity to promote antibacterial ability and epithelial growth, as well as cell proliferation and migration.Keywords: balsa, lysozyme, wound dressing, wound healing
format	article
author	Zhou D Yang T Qian W Xing M Luo G
author_facet	Zhou D Yang T Qian W Xing M Luo G
author_sort	Zhou D
title	Study of the mechanism of environmentally friendly translucent balsa-modified lysozyme dressing for facilitating wound healing
title_short	Study of the mechanism of environmentally friendly translucent balsa-modified lysozyme dressing for facilitating wound healing
title_full	Study of the mechanism of environmentally friendly translucent balsa-modified lysozyme dressing for facilitating wound healing
title_fullStr	Study of the mechanism of environmentally friendly translucent balsa-modified lysozyme dressing for facilitating wound healing
title_full_unstemmed	Study of the mechanism of environmentally friendly translucent balsa-modified lysozyme dressing for facilitating wound healing
title_sort	study of the mechanism of environmentally friendly translucent balsa-modified lysozyme dressing for facilitating wound healing
publisher	Dove Medical Press
publishDate	2018
url	https://doaj.org/article/b8894bf285b94ec09c9361c653088083
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Study of the mechanism of environmentally friendly translucent balsa-modified lysozyme dressing for facilitating wound healing

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