Biomimetic reduced graphene oxide coated collagen scaffold for in situ bone regeneration

Abstract A variety of bone-related diseases and injures and limitations of traditional regeneration methods require new tissue substitutes. Tissue engineering and regeneration combined with nanomedicine can provide different natural or synthetic and combined scaffolds with bone mimicking properties...

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Autores principales:	Sajad Bahrami, Nafiseh Baheiraei, Mostafa Shahrezaee
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b8a2e00066a247eca8dc34795b16320e
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spelling	oai:doaj.org-article:b8a2e00066a247eca8dc34795b16320e2021-12-02T16:45:41ZBiomimetic reduced graphene oxide coated collagen scaffold for in situ bone regeneration10.1038/s41598-021-96271-12045-2322https://doaj.org/article/b8a2e00066a247eca8dc34795b16320e2021-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-96271-1https://doaj.org/toc/2045-2322Abstract A variety of bone-related diseases and injures and limitations of traditional regeneration methods require new tissue substitutes. Tissue engineering and regeneration combined with nanomedicine can provide different natural or synthetic and combined scaffolds with bone mimicking properties for implantation in the injured area. In this study, we synthesized collagen (Col) and reduced graphene oxide coated collagen (Col-rGO) scaffolds, and we evaluated their in vitro and in vivo effects on bone tissue repair. Col and Col-rGO scaffolds were synthesized by chemical crosslinking and freeze-drying methods. The surface topography, and the mechanical and chemical properties of scaffolds were characterized, showing three-dimensional (3D) porous scaffolds and successful coating of rGO on Col. The rGO coating enhanced the mechanical strength of Col-rGO scaffolds to a greater extent than Col scaffolds by 2.8 times. Furthermore, Col-rGO scaffolds confirmed that graphene addition induced no cytotoxic effects and enhanced the viability and proliferation of human bone marrow-derived mesenchymal stem cells (hBMSCs) with 3D adherence and expansion. Finally, scaffold implantation into rabbit cranial bone defects for 12 weeks showed increased bone formation, confirmed by Hematoxylin–Eosin (H&E) and alizarin red staining. Overall, the study showed that rGO coating improves Col scaffold properties and could be a promising implant for bone injuries.Sajad BahramiNafiseh BaheiraeiMostafa ShahrezaeeNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sajad Bahrami Nafiseh Baheiraei Mostafa Shahrezaee Biomimetic reduced graphene oxide coated collagen scaffold for in situ bone regeneration
description	Abstract A variety of bone-related diseases and injures and limitations of traditional regeneration methods require new tissue substitutes. Tissue engineering and regeneration combined with nanomedicine can provide different natural or synthetic and combined scaffolds with bone mimicking properties for implantation in the injured area. In this study, we synthesized collagen (Col) and reduced graphene oxide coated collagen (Col-rGO) scaffolds, and we evaluated their in vitro and in vivo effects on bone tissue repair. Col and Col-rGO scaffolds were synthesized by chemical crosslinking and freeze-drying methods. The surface topography, and the mechanical and chemical properties of scaffolds were characterized, showing three-dimensional (3D) porous scaffolds and successful coating of rGO on Col. The rGO coating enhanced the mechanical strength of Col-rGO scaffolds to a greater extent than Col scaffolds by 2.8 times. Furthermore, Col-rGO scaffolds confirmed that graphene addition induced no cytotoxic effects and enhanced the viability and proliferation of human bone marrow-derived mesenchymal stem cells (hBMSCs) with 3D adherence and expansion. Finally, scaffold implantation into rabbit cranial bone defects for 12 weeks showed increased bone formation, confirmed by Hematoxylin–Eosin (H&E) and alizarin red staining. Overall, the study showed that rGO coating improves Col scaffold properties and could be a promising implant for bone injuries.
format	article
author	Sajad Bahrami Nafiseh Baheiraei Mostafa Shahrezaee
author_facet	Sajad Bahrami Nafiseh Baheiraei Mostafa Shahrezaee
author_sort	Sajad Bahrami
title	Biomimetic reduced graphene oxide coated collagen scaffold for in situ bone regeneration
title_short	Biomimetic reduced graphene oxide coated collagen scaffold for in situ bone regeneration
title_full	Biomimetic reduced graphene oxide coated collagen scaffold for in situ bone regeneration
title_fullStr	Biomimetic reduced graphene oxide coated collagen scaffold for in situ bone regeneration
title_full_unstemmed	Biomimetic reduced graphene oxide coated collagen scaffold for in situ bone regeneration
title_sort	biomimetic reduced graphene oxide coated collagen scaffold for in situ bone regeneration
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/b8a2e00066a247eca8dc34795b16320e
work_keys_str_mv	AT sajadbahrami biomimeticreducedgrapheneoxidecoatedcollagenscaffoldforinsituboneregeneration AT nafisehbaheiraei biomimeticreducedgrapheneoxidecoatedcollagenscaffoldforinsituboneregeneration AT mostafashahrezaee biomimeticreducedgrapheneoxidecoatedcollagenscaffoldforinsituboneregeneration
_version_	1718383477321105408

Biomimetic reduced graphene oxide coated collagen scaffold for in situ bone regeneration

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