Comparative RNA-seq analysis in the unsequenced axolotl: the oncogene burst highlights early gene expression in the blastema.

The salamander has the remarkable ability to regenerate its limb after amputation. Cells at the site of amputation form a blastema and then proliferate and differentiate to regrow the limb. To better understand this process, we performed deep RNA sequencing of the blastema over a time course in the...

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Autores principales: Ron Stewart, Cynthia Alexander Rascón, Shulan Tian, Jeff Nie, Chris Barry, Li-Fang Chu, Hamisha Ardalani, Ryan J Wagner, Mitchell D Probasco, Jennifer M Bolin, Ning Leng, Srikumar Sengupta, Michael Volkmer, Bianca Habermann, Elly M Tanaka, James A Thomson, Colin N Dewey
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spelling oai:doaj.org-article:b8b1636cb3834d94acff20c9e675891e2021-11-18T05:52:23ZComparative RNA-seq analysis in the unsequenced axolotl: the oncogene burst highlights early gene expression in the blastema.1553-734X1553-735810.1371/journal.pcbi.1002936https://doaj.org/article/b8b1636cb3834d94acff20c9e675891e2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23505351/pdf/?tool=EBIhttps://doaj.org/toc/1553-734Xhttps://doaj.org/toc/1553-7358The salamander has the remarkable ability to regenerate its limb after amputation. Cells at the site of amputation form a blastema and then proliferate and differentiate to regrow the limb. To better understand this process, we performed deep RNA sequencing of the blastema over a time course in the axolotl, a species whose genome has not been sequenced. Using a novel comparative approach to analyzing RNA-seq data, we characterized the transcriptional dynamics of the regenerating axolotl limb with respect to the human gene set. This approach involved de novo assembly of axolotl transcripts, RNA-seq transcript quantification without a reference genome, and transformation of abundances from axolotl contigs to human genes. We found a prominent burst in oncogene expression during the first day and blastemal/limb bud genes peaking at 7 to 14 days. In addition, we found that limb patterning genes, SALL genes, and genes involved in angiogenesis, wound healing, defense/immunity, and bone development are enriched during blastema formation and development. Finally, we identified a category of genes with no prior literature support for limb regeneration that are candidates for further evaluation based on their expression pattern during the regenerative process.Ron StewartCynthia Alexander RascónShulan TianJeff NieChris BarryLi-Fang ChuHamisha ArdalaniRyan J WagnerMitchell D ProbascoJennifer M BolinNing LengSrikumar SenguptaMichael VolkmerBianca HabermannElly M TanakaJames A ThomsonColin N DeweyPublic Library of Science (PLoS)articleBiology (General)QH301-705.5ENPLoS Computational Biology, Vol 9, Iss 3, p e1002936 (2013)
institution DOAJ
collection DOAJ
language EN
topic Biology (General)
QH301-705.5
spellingShingle Biology (General)
QH301-705.5
Ron Stewart
Cynthia Alexander Rascón
Shulan Tian
Jeff Nie
Chris Barry
Li-Fang Chu
Hamisha Ardalani
Ryan J Wagner
Mitchell D Probasco
Jennifer M Bolin
Ning Leng
Srikumar Sengupta
Michael Volkmer
Bianca Habermann
Elly M Tanaka
James A Thomson
Colin N Dewey
Comparative RNA-seq analysis in the unsequenced axolotl: the oncogene burst highlights early gene expression in the blastema.
description The salamander has the remarkable ability to regenerate its limb after amputation. Cells at the site of amputation form a blastema and then proliferate and differentiate to regrow the limb. To better understand this process, we performed deep RNA sequencing of the blastema over a time course in the axolotl, a species whose genome has not been sequenced. Using a novel comparative approach to analyzing RNA-seq data, we characterized the transcriptional dynamics of the regenerating axolotl limb with respect to the human gene set. This approach involved de novo assembly of axolotl transcripts, RNA-seq transcript quantification without a reference genome, and transformation of abundances from axolotl contigs to human genes. We found a prominent burst in oncogene expression during the first day and blastemal/limb bud genes peaking at 7 to 14 days. In addition, we found that limb patterning genes, SALL genes, and genes involved in angiogenesis, wound healing, defense/immunity, and bone development are enriched during blastema formation and development. Finally, we identified a category of genes with no prior literature support for limb regeneration that are candidates for further evaluation based on their expression pattern during the regenerative process.
format article
author Ron Stewart
Cynthia Alexander Rascón
Shulan Tian
Jeff Nie
Chris Barry
Li-Fang Chu
Hamisha Ardalani
Ryan J Wagner
Mitchell D Probasco
Jennifer M Bolin
Ning Leng
Srikumar Sengupta
Michael Volkmer
Bianca Habermann
Elly M Tanaka
James A Thomson
Colin N Dewey
author_facet Ron Stewart
Cynthia Alexander Rascón
Shulan Tian
Jeff Nie
Chris Barry
Li-Fang Chu
Hamisha Ardalani
Ryan J Wagner
Mitchell D Probasco
Jennifer M Bolin
Ning Leng
Srikumar Sengupta
Michael Volkmer
Bianca Habermann
Elly M Tanaka
James A Thomson
Colin N Dewey
author_sort Ron Stewart
title Comparative RNA-seq analysis in the unsequenced axolotl: the oncogene burst highlights early gene expression in the blastema.
title_short Comparative RNA-seq analysis in the unsequenced axolotl: the oncogene burst highlights early gene expression in the blastema.
title_full Comparative RNA-seq analysis in the unsequenced axolotl: the oncogene burst highlights early gene expression in the blastema.
title_fullStr Comparative RNA-seq analysis in the unsequenced axolotl: the oncogene burst highlights early gene expression in the blastema.
title_full_unstemmed Comparative RNA-seq analysis in the unsequenced axolotl: the oncogene burst highlights early gene expression in the blastema.
title_sort comparative rna-seq analysis in the unsequenced axolotl: the oncogene burst highlights early gene expression in the blastema.
publisher Public Library of Science (PLoS)
publishDate 2013
url https://doaj.org/article/b8b1636cb3834d94acff20c9e675891e
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