CRISPR/Cas Technologies and Their Applications in Escherichia coli

The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein (Cas) systems have revolutionized genome editing and greatly promoted the development of biotechnology. However, these systems unfortunately have not been developed and applied in bacteria as extensively...

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Autores principales:	Huina Dong, Yali Cui, Dawei Zhang
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	Escherichia coli CRISPR system transcriptional regulation knock-down knock-in Biotechnology TP248.13-248.65
Acceso en línea:	https://doaj.org/article/b8b6f4ddccb44149946bca37f51d72bb
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spelling	oai:doaj.org-article:b8b6f4ddccb44149946bca37f51d72bb2021-11-11T08:32:55ZCRISPR/Cas Technologies and Their Applications in Escherichia coli2296-418510.3389/fbioe.2021.762676https://doaj.org/article/b8b6f4ddccb44149946bca37f51d72bb2021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fbioe.2021.762676/fullhttps://doaj.org/toc/2296-4185The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein (Cas) systems have revolutionized genome editing and greatly promoted the development of biotechnology. However, these systems unfortunately have not been developed and applied in bacteria as extensively as in eukaryotic organism. Here, the research progress on the most widely used CRISPR/Cas tools and their applications in Escherichia coli is summarized. Genome editing based on homologous recombination, non-homologous DNA end-joining, transposons, and base editors are discussed. Finally, the state of the art of transcriptional regulation using CRISPRi is briefly reviewed. This review provides a useful reference for the application of CRISPR/Cas systems in other bacterial species.Huina DongHuina DongYali CuiYali CuiDawei ZhangDawei ZhangDawei ZhangFrontiers Media S.A.articleEscherichia coliCRISPR systemtranscriptional regulationknock-downknock-inBiotechnologyTP248.13-248.65ENFrontiers in Bioengineering and Biotechnology, Vol 9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Escherichia coli CRISPR system transcriptional regulation knock-down knock-in Biotechnology TP248.13-248.65
spellingShingle	Escherichia coli CRISPR system transcriptional regulation knock-down knock-in Biotechnology TP248.13-248.65 Huina Dong Huina Dong Yali Cui Yali Cui Dawei Zhang Dawei Zhang Dawei Zhang CRISPR/Cas Technologies and Their Applications in Escherichia coli
description	The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein (Cas) systems have revolutionized genome editing and greatly promoted the development of biotechnology. However, these systems unfortunately have not been developed and applied in bacteria as extensively as in eukaryotic organism. Here, the research progress on the most widely used CRISPR/Cas tools and their applications in Escherichia coli is summarized. Genome editing based on homologous recombination, non-homologous DNA end-joining, transposons, and base editors are discussed. Finally, the state of the art of transcriptional regulation using CRISPRi is briefly reviewed. This review provides a useful reference for the application of CRISPR/Cas systems in other bacterial species.
format	article
author	Huina Dong Huina Dong Yali Cui Yali Cui Dawei Zhang Dawei Zhang Dawei Zhang
author_facet	Huina Dong Huina Dong Yali Cui Yali Cui Dawei Zhang Dawei Zhang Dawei Zhang
author_sort	Huina Dong
title	CRISPR/Cas Technologies and Their Applications in Escherichia coli
title_short	CRISPR/Cas Technologies and Their Applications in Escherichia coli
title_full	CRISPR/Cas Technologies and Their Applications in Escherichia coli
title_fullStr	CRISPR/Cas Technologies and Their Applications in Escherichia coli
title_full_unstemmed	CRISPR/Cas Technologies and Their Applications in Escherichia coli
title_sort	crispr/cas technologies and their applications in escherichia coli
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/b8b6f4ddccb44149946bca37f51d72bb
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CRISPR/Cas Technologies and Their Applications in Escherichia coli

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