Influence of different elicitors on BIA production in Macleaya cordata

Abstract Sanguinarine (SAN) and chelerythrine (CHE) have been widely used as substitutes for antibiotics for decades. For a long time, SAN and CHE have been extracted from mainly Macleaya cordata, a plant species that is a traditional herb in China and belongs to the Papaveraceae family. However, wi...

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Autores principales:	Peng Huang, Liqiong Xia, Li Zhou, Wei Liu, Peng Wang, Zhixing Qing, Jianguo Zeng
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b8c0019f16e04effb882e0f617957331
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spelling	oai:doaj.org-article:b8c0019f16e04effb882e0f6179573312021-12-02T15:23:02ZInfluence of different elicitors on BIA production in Macleaya cordata10.1038/s41598-020-79802-02045-2322https://doaj.org/article/b8c0019f16e04effb882e0f6179573312021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-79802-0https://doaj.org/toc/2045-2322Abstract Sanguinarine (SAN) and chelerythrine (CHE) have been widely used as substitutes for antibiotics for decades. For a long time, SAN and CHE have been extracted from mainly Macleaya cordata, a plant species that is a traditional herb in China and belongs to the Papaveraceae family. However, with the sharp increase in demand for SAN and CHE, it is necessary to develop a new method to enhance the supply of raw materials. Here, we used methyl jasmonate (MJ), salicylic acid (SA) and wounding alone and in combination to stimulate aseptic seedlings of M. cordata at 0 h, 24 h, 72 h and 120 h and then compared the differences in metabolic profiles and gene expression. Ultimately, we found that the effect of using MJ alone was the best treatment, with the contents of SAN and CHE increasing by 10- and 14-fold, respectively. However, the increased SAN and CHE contents in response to combined wounding and MJ were less than those for induced by the treatment with MJ alone. Additionally, after MJ treatment, SAN and CHE biosynthetic pathway genes, such as those encoding the protopine 6-hydroxylase and dihydrobenzophenanthridine oxidase enzymes, were highly expressed, which is consistent with the accumulation of SAN and CHE. At the same time, we have also studied the changes in the content of synthetic intermediates of SAN and CHE after elicitor induction. This study is the first systematic research report about using elicitors to increase the SAN and CHE in Macleaya cordata.Peng HuangLiqiong XiaLi ZhouWei LiuPeng WangZhixing QingJianguo ZengNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-7 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Peng Huang Liqiong Xia Li Zhou Wei Liu Peng Wang Zhixing Qing Jianguo Zeng Influence of different elicitors on BIA production in Macleaya cordata
description	Abstract Sanguinarine (SAN) and chelerythrine (CHE) have been widely used as substitutes for antibiotics for decades. For a long time, SAN and CHE have been extracted from mainly Macleaya cordata, a plant species that is a traditional herb in China and belongs to the Papaveraceae family. However, with the sharp increase in demand for SAN and CHE, it is necessary to develop a new method to enhance the supply of raw materials. Here, we used methyl jasmonate (MJ), salicylic acid (SA) and wounding alone and in combination to stimulate aseptic seedlings of M. cordata at 0 h, 24 h, 72 h and 120 h and then compared the differences in metabolic profiles and gene expression. Ultimately, we found that the effect of using MJ alone was the best treatment, with the contents of SAN and CHE increasing by 10- and 14-fold, respectively. However, the increased SAN and CHE contents in response to combined wounding and MJ were less than those for induced by the treatment with MJ alone. Additionally, after MJ treatment, SAN and CHE biosynthetic pathway genes, such as those encoding the protopine 6-hydroxylase and dihydrobenzophenanthridine oxidase enzymes, were highly expressed, which is consistent with the accumulation of SAN and CHE. At the same time, we have also studied the changes in the content of synthetic intermediates of SAN and CHE after elicitor induction. This study is the first systematic research report about using elicitors to increase the SAN and CHE in Macleaya cordata.
format	article
author	Peng Huang Liqiong Xia Li Zhou Wei Liu Peng Wang Zhixing Qing Jianguo Zeng
author_facet	Peng Huang Liqiong Xia Li Zhou Wei Liu Peng Wang Zhixing Qing Jianguo Zeng
author_sort	Peng Huang
title	Influence of different elicitors on BIA production in Macleaya cordata
title_short	Influence of different elicitors on BIA production in Macleaya cordata
title_full	Influence of different elicitors on BIA production in Macleaya cordata
title_fullStr	Influence of different elicitors on BIA production in Macleaya cordata
title_full_unstemmed	Influence of different elicitors on BIA production in Macleaya cordata
title_sort	influence of different elicitors on bia production in macleaya cordata
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/b8c0019f16e04effb882e0f617957331
work_keys_str_mv	AT penghuang influenceofdifferentelicitorsonbiaproductioninmacleayacordata AT liqiongxia influenceofdifferentelicitorsonbiaproductioninmacleayacordata AT lizhou influenceofdifferentelicitorsonbiaproductioninmacleayacordata AT weiliu influenceofdifferentelicitorsonbiaproductioninmacleayacordata AT pengwang influenceofdifferentelicitorsonbiaproductioninmacleayacordata AT zhixingqing influenceofdifferentelicitorsonbiaproductioninmacleayacordata AT jianguozeng influenceofdifferentelicitorsonbiaproductioninmacleayacordata
_version_	1718387349841248256

Influence of different elicitors on BIA production in Macleaya cordata

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