Krill Oil-Incorporated Liposomes As An Effective Nanovehicle To Ameliorate The Inflammatory Responses Of DSS-Induced Colitis

Jin-Hee Kim,1,* Soon-Seok Hong,1,* Myoungsoo Lee,2 Eun-Hye Lee,1 Inmoo Rhee,1 Sun-Young Chang,2 Soo-Jeong Lim1 1Department of Integrated Bioscience and Biotechnology, Sejong University, Seoul 05006, Republic of Korea; 2Laboratory of Microbiology, College of Pharmacy, and Research Institute of Pharma...

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Autores principales:	Kim JH, Hong SS, Lee M, Lee EH, Rhee I, Chang SY, Lim SJ
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2019
Materias:	inflammatory bowel disease phosphatidylcholine liposomes krill oil omega-3 fatty acid Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/b8cab3d68cc34f388686e10c1c750ad1
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spelling	oai:doaj.org-article:b8cab3d68cc34f388686e10c1c750ad12021-12-02T11:26:41ZKrill Oil-Incorporated Liposomes As An Effective Nanovehicle To Ameliorate The Inflammatory Responses Of DSS-Induced Colitis1178-2013https://doaj.org/article/b8cab3d68cc34f388686e10c1c750ad12019-11-01T00:00:00Zhttps://www.dovepress.com/krill-oil-incorporated-liposomes-as-an-effective-nanovehicle-to-amelio-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Jin-Hee Kim,1,* Soon-Seok Hong,1,* Myoungsoo Lee,2 Eun-Hye Lee,1 Inmoo Rhee,1 Sun-Young Chang,2 Soo-Jeong Lim1 1Department of Integrated Bioscience and Biotechnology, Sejong University, Seoul 05006, Republic of Korea; 2Laboratory of Microbiology, College of Pharmacy, and Research Institute of Pharmaceutical Science and Technology (RIPST), Ajou University, Suwon 16499, Republic of Korea*These authors contributed equally to this workCorrespondence: Sun-Young ChangLaboratory of Microbiology, College of Pharmacy, and Research Institute of Pharmaceutical Science and Technology (RIPST), Ajou University, Suwon 16499, Republic of KoreaTel +82 31 219 3454Fax +82 31 219 3435Email sychang@ajou.ac.krSoo-Jeong LimDepartment of Integrated Bioscience and Biotechnology, Sejong University, 209 Neungdong-ro, Gwangjin-gu, Seoul 05006, Republic of KoreaTel +82 2 3408 3767Fax +82 2 3408 4334Email sjlim@sejong.ac.krBackground: Phosphatidylcholine (PC) and Omega-3 fatty acid (Omega-3) are promising therapeutic molecules for treating inflammatory bowel disease (IBD).Purpose: Based on the IBD therapeutic potential of nanoparticles, we herein sought to develop Omega-3-incorporated PC nanoparticles (liposomes) as an orally administrable vehicle for treating IBD.Methods: Liposomes prepared with or without Omega-3 incorporation were compared in terms of colloidal stability and anitiinflammatory effects.Results: The incorporation of free Omega-3 (alpha-linolenic acid, eicosapentaenoic acid or docosahexaenoic acid) into liposomes induced time-dependent membrane fusion, resulting in particle size increase from nm to μm during storage. In contrast, krill oil incorporation into liposomes (KO liposomes) did not induce the fusion and the particle size maintained <250 nm during storage. KO liposomes also maintained colloidal stability in simulated gastrointestinal conditions and exhibited a high capacity to entrap the IBD drug, budesonide (BDS). KO liposomes greatly suppressed the lipopolysaccharide-induced production of pro-inflammatory cytokines in cultured macrophages and completely restored inflammation-impaired membrane barrier function in an intestinal barrier model. In mice subjected to dextran sulfate sodium-induced colitis, oral administration of BDS-entrapped KO liposomes suppressed tumor necrosis factor-α production (by 84.1%), interleukin-6 production (by 35.3%), and the systemic level of endotoxin (by 96.8%), and slightly reduced the macroscopic signs of the disease.Conclusion: Taken together, KO liposomes may have great potential as a nanovehicle for oral delivery of IBD drugs.Keywords: inflammatory bowel disease, phosphatidylcholine, liposomes, krill oil, Omega-3 fatty acidKim JHHong SSLee MLee EHRhee IChang SYLim SJDove Medical Pressarticleinflammatory bowel diseasephosphatidylcholineliposomeskrill oilomega-3 fatty acidMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 14, Pp 8305-8320 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	inflammatory bowel disease phosphatidylcholine liposomes krill oil omega-3 fatty acid Medicine (General) R5-920
spellingShingle	inflammatory bowel disease phosphatidylcholine liposomes krill oil omega-3 fatty acid Medicine (General) R5-920 Kim JH Hong SS Lee M Lee EH Rhee I Chang SY Lim SJ Krill Oil-Incorporated Liposomes As An Effective Nanovehicle To Ameliorate The Inflammatory Responses Of DSS-Induced Colitis
description	Jin-Hee Kim,1,* Soon-Seok Hong,1,* Myoungsoo Lee,2 Eun-Hye Lee,1 Inmoo Rhee,1 Sun-Young Chang,2 Soo-Jeong Lim1 1Department of Integrated Bioscience and Biotechnology, Sejong University, Seoul 05006, Republic of Korea; 2Laboratory of Microbiology, College of Pharmacy, and Research Institute of Pharmaceutical Science and Technology (RIPST), Ajou University, Suwon 16499, Republic of Korea*These authors contributed equally to this workCorrespondence: Sun-Young ChangLaboratory of Microbiology, College of Pharmacy, and Research Institute of Pharmaceutical Science and Technology (RIPST), Ajou University, Suwon 16499, Republic of KoreaTel +82 31 219 3454Fax +82 31 219 3435Email sychang@ajou.ac.krSoo-Jeong LimDepartment of Integrated Bioscience and Biotechnology, Sejong University, 209 Neungdong-ro, Gwangjin-gu, Seoul 05006, Republic of KoreaTel +82 2 3408 3767Fax +82 2 3408 4334Email sjlim@sejong.ac.krBackground: Phosphatidylcholine (PC) and Omega-3 fatty acid (Omega-3) are promising therapeutic molecules for treating inflammatory bowel disease (IBD).Purpose: Based on the IBD therapeutic potential of nanoparticles, we herein sought to develop Omega-3-incorporated PC nanoparticles (liposomes) as an orally administrable vehicle for treating IBD.Methods: Liposomes prepared with or without Omega-3 incorporation were compared in terms of colloidal stability and anitiinflammatory effects.Results: The incorporation of free Omega-3 (alpha-linolenic acid, eicosapentaenoic acid or docosahexaenoic acid) into liposomes induced time-dependent membrane fusion, resulting in particle size increase from nm to μm during storage. In contrast, krill oil incorporation into liposomes (KO liposomes) did not induce the fusion and the particle size maintained <250 nm during storage. KO liposomes also maintained colloidal stability in simulated gastrointestinal conditions and exhibited a high capacity to entrap the IBD drug, budesonide (BDS). KO liposomes greatly suppressed the lipopolysaccharide-induced production of pro-inflammatory cytokines in cultured macrophages and completely restored inflammation-impaired membrane barrier function in an intestinal barrier model. In mice subjected to dextran sulfate sodium-induced colitis, oral administration of BDS-entrapped KO liposomes suppressed tumor necrosis factor-α production (by 84.1%), interleukin-6 production (by 35.3%), and the systemic level of endotoxin (by 96.8%), and slightly reduced the macroscopic signs of the disease.Conclusion: Taken together, KO liposomes may have great potential as a nanovehicle for oral delivery of IBD drugs.Keywords: inflammatory bowel disease, phosphatidylcholine, liposomes, krill oil, Omega-3 fatty acid
format	article
author	Kim JH Hong SS Lee M Lee EH Rhee I Chang SY Lim SJ
author_facet	Kim JH Hong SS Lee M Lee EH Rhee I Chang SY Lim SJ
author_sort	Kim JH
title	Krill Oil-Incorporated Liposomes As An Effective Nanovehicle To Ameliorate The Inflammatory Responses Of DSS-Induced Colitis
title_short	Krill Oil-Incorporated Liposomes As An Effective Nanovehicle To Ameliorate The Inflammatory Responses Of DSS-Induced Colitis
title_full	Krill Oil-Incorporated Liposomes As An Effective Nanovehicle To Ameliorate The Inflammatory Responses Of DSS-Induced Colitis
title_fullStr	Krill Oil-Incorporated Liposomes As An Effective Nanovehicle To Ameliorate The Inflammatory Responses Of DSS-Induced Colitis
title_full_unstemmed	Krill Oil-Incorporated Liposomes As An Effective Nanovehicle To Ameliorate The Inflammatory Responses Of DSS-Induced Colitis
title_sort	krill oil-incorporated liposomes as an effective nanovehicle to ameliorate the inflammatory responses of dss-induced colitis
publisher	Dove Medical Press
publishDate	2019
url	https://doaj.org/article/b8cab3d68cc34f388686e10c1c750ad1
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Krill Oil-Incorporated Liposomes As An Effective Nanovehicle To Ameliorate The Inflammatory Responses Of DSS-Induced Colitis

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