TbMYC4A Is a Candidate Gene Controlling the Blue Aleurone Trait in a Wheat-Triticum boeoticum Substitution Line
Triticum boeoticum Boiss (AbAb, 2n = 2x = 14) is one of the sources of the blue grain trait controlled by blue aleurone layer 2 (Ba2). However, the underlying genes have not been cloned. In this study, a transcriptomic comparison between a blue-grained wheat-T. boeoticum substitution line and its wh...
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Frontiers Media S.A.
2021
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oai:doaj.org-article:b8d407ba6c6f48c789d7d8a99496fed72021-11-05T16:33:10ZTbMYC4A Is a Candidate Gene Controlling the Blue Aleurone Trait in a Wheat-Triticum boeoticum Substitution Line1664-462X10.3389/fpls.2021.762265https://doaj.org/article/b8d407ba6c6f48c789d7d8a99496fed72021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fpls.2021.762265/fullhttps://doaj.org/toc/1664-462XTriticum boeoticum Boiss (AbAb, 2n = 2x = 14) is one of the sources of the blue grain trait controlled by blue aleurone layer 2 (Ba2). However, the underlying genes have not been cloned. In this study, a transcriptomic comparison between a blue-grained wheat-T. boeoticum substitution line and its wheat parent identified 41 unigenes related to anthocyanin biosynthesis and 29 unigenes related to transport. The bHLH transcription factor gene TbMYC4A showed a higher expression level in the blue-grained substitution line. TbMYC4A contained the three characteristic bHLH transcription factor domains (bHLH-MYC_N, HLH and ACT-like) and clustered with genes identified from other wheat lines with the blue grain trait derived from other Triticeae species. TbMYC4A overexpression confirmed that it was a functional bHLH transcription factor. The analysis of a TbMYC4A-specific marker showed that the gene was also present in T. boeoticum and T. monococcum with blue aleurone but absent in other Triticeae materials with white aleurone. These results indicate that TbMYC4A is a candidate gene of Ba2 controlling the blue aleurone trait. The isolation of TbMYC4A is helpful for further clarifying the genetic mechanism of the blue aleurone trait and is of great significance for breeding blue-grained wheat varieties.Xin LiuMinghu ZhangXiaomei JiangHui LiZhenjiao JiaMing HaoBo JiangLin HuangLin HuangShunzong NingZhongwei YuanXuejiao ChenXue ChenDengcai LiuDengcai LiuBaolong LiuBaolong LiuLianquan ZhangLianquan ZhangFrontiers Media S.A.articleTriticum boeoticumanthocyanin biosynthesisblue aleuronetranscriptome analysisTbMYC4APlant cultureSB1-1110ENFrontiers in Plant Science, Vol 12 (2021) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
Triticum boeoticum anthocyanin biosynthesis blue aleurone transcriptome analysis TbMYC4A Plant culture SB1-1110 |
| spellingShingle |
Triticum boeoticum anthocyanin biosynthesis blue aleurone transcriptome analysis TbMYC4A Plant culture SB1-1110 Xin Liu Minghu Zhang Xiaomei Jiang Hui Li Zhenjiao Jia Ming Hao Bo Jiang Lin Huang Lin Huang Shunzong Ning Zhongwei Yuan Xuejiao Chen Xue Chen Dengcai Liu Dengcai Liu Baolong Liu Baolong Liu Lianquan Zhang Lianquan Zhang TbMYC4A Is a Candidate Gene Controlling the Blue Aleurone Trait in a Wheat-Triticum boeoticum Substitution Line |
| description |
Triticum boeoticum Boiss (AbAb, 2n = 2x = 14) is one of the sources of the blue grain trait controlled by blue aleurone layer 2 (Ba2). However, the underlying genes have not been cloned. In this study, a transcriptomic comparison between a blue-grained wheat-T. boeoticum substitution line and its wheat parent identified 41 unigenes related to anthocyanin biosynthesis and 29 unigenes related to transport. The bHLH transcription factor gene TbMYC4A showed a higher expression level in the blue-grained substitution line. TbMYC4A contained the three characteristic bHLH transcription factor domains (bHLH-MYC_N, HLH and ACT-like) and clustered with genes identified from other wheat lines with the blue grain trait derived from other Triticeae species. TbMYC4A overexpression confirmed that it was a functional bHLH transcription factor. The analysis of a TbMYC4A-specific marker showed that the gene was also present in T. boeoticum and T. monococcum with blue aleurone but absent in other Triticeae materials with white aleurone. These results indicate that TbMYC4A is a candidate gene of Ba2 controlling the blue aleurone trait. The isolation of TbMYC4A is helpful for further clarifying the genetic mechanism of the blue aleurone trait and is of great significance for breeding blue-grained wheat varieties. |
| format |
article |
| author |
Xin Liu Minghu Zhang Xiaomei Jiang Hui Li Zhenjiao Jia Ming Hao Bo Jiang Lin Huang Lin Huang Shunzong Ning Zhongwei Yuan Xuejiao Chen Xue Chen Dengcai Liu Dengcai Liu Baolong Liu Baolong Liu Lianquan Zhang Lianquan Zhang |
| author_facet |
Xin Liu Minghu Zhang Xiaomei Jiang Hui Li Zhenjiao Jia Ming Hao Bo Jiang Lin Huang Lin Huang Shunzong Ning Zhongwei Yuan Xuejiao Chen Xue Chen Dengcai Liu Dengcai Liu Baolong Liu Baolong Liu Lianquan Zhang Lianquan Zhang |
| author_sort |
Xin Liu |
| title |
TbMYC4A Is a Candidate Gene Controlling the Blue Aleurone Trait in a Wheat-Triticum boeoticum Substitution Line |
| title_short |
TbMYC4A Is a Candidate Gene Controlling the Blue Aleurone Trait in a Wheat-Triticum boeoticum Substitution Line |
| title_full |
TbMYC4A Is a Candidate Gene Controlling the Blue Aleurone Trait in a Wheat-Triticum boeoticum Substitution Line |
| title_fullStr |
TbMYC4A Is a Candidate Gene Controlling the Blue Aleurone Trait in a Wheat-Triticum boeoticum Substitution Line |
| title_full_unstemmed |
TbMYC4A Is a Candidate Gene Controlling the Blue Aleurone Trait in a Wheat-Triticum boeoticum Substitution Line |
| title_sort |
tbmyc4a is a candidate gene controlling the blue aleurone trait in a wheat-triticum boeoticum substitution line |
| publisher |
Frontiers Media S.A. |
| publishDate |
2021 |
| url |
https://doaj.org/article/b8d407ba6c6f48c789d7d8a99496fed7 |
| work_keys_str_mv |
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