Genotoxicity and oxidative stress induction by polystyrene nanoparticles in the colorectal cancer cell line HCT116.

The potential risks of environmental nanoparticles (NPs), in particular Polystyrene Nanoparticles (PNPs), is an emerging problem; specifically, the interaction of PNPs with intestinal cells has not been characterized so far. The mechanism by which polystyrene particles are transferred to humans has...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Giulia Vecchiotti, Sabrina Colafarina, Massimo Aloisi, Osvaldo Zarivi, Piero Di Carlo, Anna Poma
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b8d57e35852d47faa8602d6b02e2e744
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:b8d57e35852d47faa8602d6b02e2e744
record_format	dspace
spelling	oai:doaj.org-article:b8d57e35852d47faa8602d6b02e2e7442021-12-02T20:06:29ZGenotoxicity and oxidative stress induction by polystyrene nanoparticles in the colorectal cancer cell line HCT116.1932-620310.1371/journal.pone.0255120https://doaj.org/article/b8d57e35852d47faa8602d6b02e2e7442021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0255120https://doaj.org/toc/1932-6203The potential risks of environmental nanoparticles (NPs), in particular Polystyrene Nanoparticles (PNPs), is an emerging problem; specifically, the interaction of PNPs with intestinal cells has not been characterized so far. The mechanism by which polystyrene particles are transferred to humans has not yet been clarified, whether directly through ingestion from contaminated food. We evaluated the interaction between PNPs and colorectal adenocarcinoma cells (HCT116). Cells were exposed to different concentrations of PNPs, metabolic activity and the consequent cytotoxic potential were assessed through viability test; we evaluated the PNP genotoxic potential through the Cytokinesis-Block Micronucleus cytome (CBMN cyt) assay. Finally, we detected Reactive Oxygen Species (ROS) production after NPs exposure and performed Western Blot analysis to analyze the enzymes (SOD1, SOD2, Catalase, Glutathione Peroxidase) involved in the cell detoxification process that comes into play during the cell-PNPs interaction. This work analyzes the cyto and genotoxicity of PNPs in the colorectal HCT116 cell line, in particular the potential damage from oxidative stress produced by PNPs inside the cells related to the consequent nuclear damage. Our results show moderate toxicity of PNPs both in terms of ROS production and DNA damage. Further studies will be needed on different cell lines to have a more complete picture of the impact of environmental pollution on human health in terms of PNPs cytotoxicity and genotoxicity.Giulia VecchiottiSabrina ColafarinaMassimo AloisiOsvaldo ZariviPiero Di CarloAnna PomaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 7, p e0255120 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Giulia Vecchiotti Sabrina Colafarina Massimo Aloisi Osvaldo Zarivi Piero Di Carlo Anna Poma Genotoxicity and oxidative stress induction by polystyrene nanoparticles in the colorectal cancer cell line HCT116.
description	The potential risks of environmental nanoparticles (NPs), in particular Polystyrene Nanoparticles (PNPs), is an emerging problem; specifically, the interaction of PNPs with intestinal cells has not been characterized so far. The mechanism by which polystyrene particles are transferred to humans has not yet been clarified, whether directly through ingestion from contaminated food. We evaluated the interaction between PNPs and colorectal adenocarcinoma cells (HCT116). Cells were exposed to different concentrations of PNPs, metabolic activity and the consequent cytotoxic potential were assessed through viability test; we evaluated the PNP genotoxic potential through the Cytokinesis-Block Micronucleus cytome (CBMN cyt) assay. Finally, we detected Reactive Oxygen Species (ROS) production after NPs exposure and performed Western Blot analysis to analyze the enzymes (SOD1, SOD2, Catalase, Glutathione Peroxidase) involved in the cell detoxification process that comes into play during the cell-PNPs interaction. This work analyzes the cyto and genotoxicity of PNPs in the colorectal HCT116 cell line, in particular the potential damage from oxidative stress produced by PNPs inside the cells related to the consequent nuclear damage. Our results show moderate toxicity of PNPs both in terms of ROS production and DNA damage. Further studies will be needed on different cell lines to have a more complete picture of the impact of environmental pollution on human health in terms of PNPs cytotoxicity and genotoxicity.
format	article
author	Giulia Vecchiotti Sabrina Colafarina Massimo Aloisi Osvaldo Zarivi Piero Di Carlo Anna Poma
author_facet	Giulia Vecchiotti Sabrina Colafarina Massimo Aloisi Osvaldo Zarivi Piero Di Carlo Anna Poma
author_sort	Giulia Vecchiotti
title	Genotoxicity and oxidative stress induction by polystyrene nanoparticles in the colorectal cancer cell line HCT116.
title_short	Genotoxicity and oxidative stress induction by polystyrene nanoparticles in the colorectal cancer cell line HCT116.
title_full	Genotoxicity and oxidative stress induction by polystyrene nanoparticles in the colorectal cancer cell line HCT116.
title_fullStr	Genotoxicity and oxidative stress induction by polystyrene nanoparticles in the colorectal cancer cell line HCT116.
title_full_unstemmed	Genotoxicity and oxidative stress induction by polystyrene nanoparticles in the colorectal cancer cell line HCT116.
title_sort	genotoxicity and oxidative stress induction by polystyrene nanoparticles in the colorectal cancer cell line hct116.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/b8d57e35852d47faa8602d6b02e2e744
work_keys_str_mv	AT giuliavecchiotti genotoxicityandoxidativestressinductionbypolystyrenenanoparticlesinthecolorectalcancercelllinehct116 AT sabrinacolafarina genotoxicityandoxidativestressinductionbypolystyrenenanoparticlesinthecolorectalcancercelllinehct116 AT massimoaloisi genotoxicityandoxidativestressinductionbypolystyrenenanoparticlesinthecolorectalcancercelllinehct116 AT osvaldozarivi genotoxicityandoxidativestressinductionbypolystyrenenanoparticlesinthecolorectalcancercelllinehct116 AT pierodicarlo genotoxicityandoxidativestressinductionbypolystyrenenanoparticlesinthecolorectalcancercelllinehct116 AT annapoma genotoxicityandoxidativestressinductionbypolystyrenenanoparticlesinthecolorectalcancercelllinehct116
_version_	1718375383706894336

Genotoxicity and oxidative stress induction by polystyrene nanoparticles in the colorectal cancer cell line HCT116.

Ejemplares similares