Low-dose Drosera rotundifolia induces gene expression changes in 16HBE human bronchial epithelial cells

Abstract Drosera rotundifolia has been traditionally used for the treatment of respiratory diseases in phytotherapy and homeopathy. The mechanisms of action recognized so far are linked to the known effects of specific components, such as flavonoids, but are not completely understood. In this study,...

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Autores principales:	Fabio Arruda-Silva, Paolo Bellavite, Marta Marzotto
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b8df4022f0c2406fb8921d9632ede5d6
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spelling	oai:doaj.org-article:b8df4022f0c2406fb8921d9632ede5d62021-12-02T13:24:26ZLow-dose Drosera rotundifolia induces gene expression changes in 16HBE human bronchial epithelial cells10.1038/s41598-021-81843-y2045-2322https://doaj.org/article/b8df4022f0c2406fb8921d9632ede5d62021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-81843-yhttps://doaj.org/toc/2045-2322Abstract Drosera rotundifolia has been traditionally used for the treatment of respiratory diseases in phytotherapy and homeopathy. The mechanisms of action recognized so far are linked to the known effects of specific components, such as flavonoids, but are not completely understood. In this study, the biological functions of D. rotundifolia were explored in vitro following the treatment of bronchial epithelial cells, which are the potential targets of the pharmacological effects of the herbal medicine. To do so, the whole plant ethanolic extract was 1000-fold diluted in water (D. rotundifolia 3×) and added to a 16HBE human cell line culture for 3 h or 6 h. The effects on gene expression of the treatments and corresponding controls were then investigated by RNA sequencing. The differentially expressed genes were validated through RT-qPCR, and the enriched biological functions involved in the effects of treatment were investigated. D. rotundifolia 3× did not impair cell viability and was shown to be a stimulant of cell functions by regulating the expression of dozens of genes after 3 h, and the effects were amplified after 6 h of treatment. The main differentially expressed genes encoded ligands of epithelial growth factor receptor, proteins involved in xenobiotic detoxification and cytokines, suggesting that D. rotundifolia 3× could stimulate self-repair systems, which are impaired in airway diseases. Furthermore, D. rotundifolia 3× acts on a complex and multifaceted set of genes and may potentially affect different layers of the bronchial mucosa.Fabio Arruda-SilvaPaolo BellaviteMarta MarzottoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-15 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Fabio Arruda-Silva Paolo Bellavite Marta Marzotto Low-dose Drosera rotundifolia induces gene expression changes in 16HBE human bronchial epithelial cells
description	Abstract Drosera rotundifolia has been traditionally used for the treatment of respiratory diseases in phytotherapy and homeopathy. The mechanisms of action recognized so far are linked to the known effects of specific components, such as flavonoids, but are not completely understood. In this study, the biological functions of D. rotundifolia were explored in vitro following the treatment of bronchial epithelial cells, which are the potential targets of the pharmacological effects of the herbal medicine. To do so, the whole plant ethanolic extract was 1000-fold diluted in water (D. rotundifolia 3×) and added to a 16HBE human cell line culture for 3 h or 6 h. The effects on gene expression of the treatments and corresponding controls were then investigated by RNA sequencing. The differentially expressed genes were validated through RT-qPCR, and the enriched biological functions involved in the effects of treatment were investigated. D. rotundifolia 3× did not impair cell viability and was shown to be a stimulant of cell functions by regulating the expression of dozens of genes after 3 h, and the effects were amplified after 6 h of treatment. The main differentially expressed genes encoded ligands of epithelial growth factor receptor, proteins involved in xenobiotic detoxification and cytokines, suggesting that D. rotundifolia 3× could stimulate self-repair systems, which are impaired in airway diseases. Furthermore, D. rotundifolia 3× acts on a complex and multifaceted set of genes and may potentially affect different layers of the bronchial mucosa.
format	article
author	Fabio Arruda-Silva Paolo Bellavite Marta Marzotto
author_facet	Fabio Arruda-Silva Paolo Bellavite Marta Marzotto
author_sort	Fabio Arruda-Silva
title	Low-dose Drosera rotundifolia induces gene expression changes in 16HBE human bronchial epithelial cells
title_short	Low-dose Drosera rotundifolia induces gene expression changes in 16HBE human bronchial epithelial cells
title_full	Low-dose Drosera rotundifolia induces gene expression changes in 16HBE human bronchial epithelial cells
title_fullStr	Low-dose Drosera rotundifolia induces gene expression changes in 16HBE human bronchial epithelial cells
title_full_unstemmed	Low-dose Drosera rotundifolia induces gene expression changes in 16HBE human bronchial epithelial cells
title_sort	low-dose drosera rotundifolia induces gene expression changes in 16hbe human bronchial epithelial cells
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/b8df4022f0c2406fb8921d9632ede5d6
work_keys_str_mv	AT fabioarrudasilva lowdosedroserarotundifoliainducesgeneexpressionchangesin16hbehumanbronchialepithelialcells AT paolobellavite lowdosedroserarotundifoliainducesgeneexpressionchangesin16hbehumanbronchialepithelialcells AT martamarzotto lowdosedroserarotundifoliainducesgeneexpressionchangesin16hbehumanbronchialepithelialcells
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Low-dose Drosera rotundifolia induces gene expression changes in 16HBE human bronchial epithelial cells

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