Mutations in the IMD Pathway and Mustard Counter <named-content content-type="genus-species">Vibrio cholerae</named-content> Suppression of Intestinal Stem Cell Division in <italic toggle="yes">Drosophila</italic>

ABSTRACT Vibrio cholerae is an estuarine bacterium and an intestinal pathogen of humans that causes severe epidemic diarrhea. In the absence of adequate mammalian models in which to study the interaction of V. cholerae with the host intestinal innate immune system, we have implemented Drosophila mel...

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Autores principales:	Zhipeng Wang, Saiyu Hang, Alexandra E. Purdy, Paula I. Watnick
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2013
Materias:	Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/b8e4838e21f34963b52675c28363f6fd
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spelling	oai:doaj.org-article:b8e4838e21f34963b52675c28363f6fd2021-11-15T15:40:05ZMutations in the IMD Pathway and Mustard Counter <named-content content-type="genus-species">Vibrio cholerae</named-content> Suppression of Intestinal Stem Cell Division in <italic toggle="yes">Drosophila</italic>10.1128/mBio.00337-132150-7511https://doaj.org/article/b8e4838e21f34963b52675c28363f6fd2013-07-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mBio.00337-13https://doaj.org/toc/2150-7511ABSTRACT Vibrio cholerae is an estuarine bacterium and an intestinal pathogen of humans that causes severe epidemic diarrhea. In the absence of adequate mammalian models in which to study the interaction of V. cholerae with the host intestinal innate immune system, we have implemented Drosophila melanogaster as a surrogate host. We previously showed that immune deficiency pathway loss-of-function and mustard gain-of-function mutants are less susceptible to V. cholerae infection. We find that although the overall burden of intestinal bacteria is not significantly different from that of control flies, intestinal stem cell (ISC) division is increased in these mutants. This led us to examine the effect of V. cholerae on ISC division. We report that V. cholerae infection and cholera toxin decrease ISC division. Because IMD pathway and Mustard mutants, which are resistant to V. cholerae, maintain higher levels of ISC division during V. cholerae infection, we hypothesize that suppression of ISC division is a virulence strategy of V. cholerae and that accelerated epithelial regeneration protects the host against V. cholerae. Extension of these findings to mammals awaits the development of an adequate experimental model. IMPORTANCE Here we show that Vibrio cholerae and cholera toxin suppress intestinal stem cell (ISC) division. This is the first evidence of manipulation of ISC division by V. cholerae and demonstrates the utility of the Drosophila model in generating novel hypotheses regarding the interaction of V. cholerae with the intestinal epithelium. Furthermore, we add to the body of data suggesting that the IMD pathway and the Mustard protein modulate ISC division independently of the overall load of commensal intestinal bacteria.Zhipeng WangSaiyu HangAlexandra E. PurdyPaula I. WatnickAmerican Society for MicrobiologyarticleMicrobiologyQR1-502ENmBio, Vol 4, Iss 3 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Microbiology QR1-502
spellingShingle	Microbiology QR1-502 Zhipeng Wang Saiyu Hang Alexandra E. Purdy Paula I. Watnick Mutations in the IMD Pathway and Mustard Counter <named-content content-type="genus-species">Vibrio cholerae</named-content> Suppression of Intestinal Stem Cell Division in <italic toggle="yes">Drosophila</italic>
description	ABSTRACT Vibrio cholerae is an estuarine bacterium and an intestinal pathogen of humans that causes severe epidemic diarrhea. In the absence of adequate mammalian models in which to study the interaction of V. cholerae with the host intestinal innate immune system, we have implemented Drosophila melanogaster as a surrogate host. We previously showed that immune deficiency pathway loss-of-function and mustard gain-of-function mutants are less susceptible to V. cholerae infection. We find that although the overall burden of intestinal bacteria is not significantly different from that of control flies, intestinal stem cell (ISC) division is increased in these mutants. This led us to examine the effect of V. cholerae on ISC division. We report that V. cholerae infection and cholera toxin decrease ISC division. Because IMD pathway and Mustard mutants, which are resistant to V. cholerae, maintain higher levels of ISC division during V. cholerae infection, we hypothesize that suppression of ISC division is a virulence strategy of V. cholerae and that accelerated epithelial regeneration protects the host against V. cholerae. Extension of these findings to mammals awaits the development of an adequate experimental model. IMPORTANCE Here we show that Vibrio cholerae and cholera toxin suppress intestinal stem cell (ISC) division. This is the first evidence of manipulation of ISC division by V. cholerae and demonstrates the utility of the Drosophila model in generating novel hypotheses regarding the interaction of V. cholerae with the intestinal epithelium. Furthermore, we add to the body of data suggesting that the IMD pathway and the Mustard protein modulate ISC division independently of the overall load of commensal intestinal bacteria.
format	article
author	Zhipeng Wang Saiyu Hang Alexandra E. Purdy Paula I. Watnick
author_facet	Zhipeng Wang Saiyu Hang Alexandra E. Purdy Paula I. Watnick
author_sort	Zhipeng Wang
title	Mutations in the IMD Pathway and Mustard Counter <named-content content-type="genus-species">Vibrio cholerae</named-content> Suppression of Intestinal Stem Cell Division in <italic toggle="yes">Drosophila</italic>
title_short	Mutations in the IMD Pathway and Mustard Counter <named-content content-type="genus-species">Vibrio cholerae</named-content> Suppression of Intestinal Stem Cell Division in <italic toggle="yes">Drosophila</italic>
title_full	Mutations in the IMD Pathway and Mustard Counter <named-content content-type="genus-species">Vibrio cholerae</named-content> Suppression of Intestinal Stem Cell Division in <italic toggle="yes">Drosophila</italic>
title_fullStr	Mutations in the IMD Pathway and Mustard Counter <named-content content-type="genus-species">Vibrio cholerae</named-content> Suppression of Intestinal Stem Cell Division in <italic toggle="yes">Drosophila</italic>
title_full_unstemmed	Mutations in the IMD Pathway and Mustard Counter <named-content content-type="genus-species">Vibrio cholerae</named-content> Suppression of Intestinal Stem Cell Division in <italic toggle="yes">Drosophila</italic>
title_sort	mutations in the imd pathway and mustard counter <named-content content-type="genus-species">vibrio cholerae</named-content> suppression of intestinal stem cell division in <italic toggle="yes">drosophila</italic>
publisher	American Society for Microbiology
publishDate	2013
url	https://doaj.org/article/b8e4838e21f34963b52675c28363f6fd
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Mutations in the IMD Pathway and Mustard Counter <named-content content-type="genus-species">Vibrio cholerae</named-content> Suppression of Intestinal Stem Cell Division in <italic toggle="yes">Drosophila</italic>

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