Neurosurgical Approaches to Brain Tissue Harvesting for the Establishment of Cell Cultures in Neural Experimental Cell Models

In recent decades, cell biology has made rapid progress. Cell isolation and cultivation techniques, supported by modern laboratory procedures and experimental capabilities, provide a wide range of opportunities for in vitro research to study physiological and pathophysiological processes in health a...

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Autores principales:	Lidija Gradišnik, Roman Bošnjak, Gorazd Bunc, Janez Ravnik, Tina Maver, Tomaž Velnar
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	cell isolation brain experimental cell models biomaterial testing neurosurgery Technology T Electrical engineering. Electronics. Nuclear engineering TK1-9971 Engineering (General). Civil engineering (General) TA1-2040 Microscopy QH201-278.5 Descriptive and experimental mechanics QC120-168.85
Acceso en línea:	https://doaj.org/article/b8e81d68b99a47c78766142815129b4a
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spelling	oai:doaj.org-article:b8e81d68b99a47c78766142815129b4a2021-11-25T18:14:10ZNeurosurgical Approaches to Brain Tissue Harvesting for the Establishment of Cell Cultures in Neural Experimental Cell Models10.3390/ma142268571996-1944https://doaj.org/article/b8e81d68b99a47c78766142815129b4a2021-11-01T00:00:00Zhttps://www.mdpi.com/1996-1944/14/22/6857https://doaj.org/toc/1996-1944In recent decades, cell biology has made rapid progress. Cell isolation and cultivation techniques, supported by modern laboratory procedures and experimental capabilities, provide a wide range of opportunities for in vitro research to study physiological and pathophysiological processes in health and disease. They can also be used very efficiently for the analysis of biomaterials. Before a new biomaterial is ready for implantation into tissues and widespread use in clinical practice, it must be extensively tested. Experimental cell models, which are a suitable testing ground and the first line of empirical exploration of new biomaterials, must contain suitable cells that form the basis of biomaterial testing. To isolate a stable and suitable cell culture, many steps are required. The first and one of the most important steps is the collection of donor tissue, usually during a surgical procedure. Thus, the collection is the foundation for the success of cell isolation. This article explains the sources and neurosurgical procedures for obtaining brain tissue samples for cell isolation techniques, which are essential for biomaterial testing procedures.Lidija GradišnikRoman BošnjakGorazd BuncJanez RavnikTina MaverTomaž VelnarMDPI AGarticlecell isolationbrainexperimental cell modelsbiomaterial testingneurosurgeryTechnologyTElectrical engineering. Electronics. Nuclear engineeringTK1-9971Engineering (General). Civil engineering (General)TA1-2040MicroscopyQH201-278.5Descriptive and experimental mechanicsQC120-168.85ENMaterials, Vol 14, Iss 6857, p 6857 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	cell isolation brain experimental cell models biomaterial testing neurosurgery Technology T Electrical engineering. Electronics. Nuclear engineering TK1-9971 Engineering (General). Civil engineering (General) TA1-2040 Microscopy QH201-278.5 Descriptive and experimental mechanics QC120-168.85
spellingShingle	cell isolation brain experimental cell models biomaterial testing neurosurgery Technology T Electrical engineering. Electronics. Nuclear engineering TK1-9971 Engineering (General). Civil engineering (General) TA1-2040 Microscopy QH201-278.5 Descriptive and experimental mechanics QC120-168.85 Lidija Gradišnik Roman Bošnjak Gorazd Bunc Janez Ravnik Tina Maver Tomaž Velnar Neurosurgical Approaches to Brain Tissue Harvesting for the Establishment of Cell Cultures in Neural Experimental Cell Models
description	In recent decades, cell biology has made rapid progress. Cell isolation and cultivation techniques, supported by modern laboratory procedures and experimental capabilities, provide a wide range of opportunities for in vitro research to study physiological and pathophysiological processes in health and disease. They can also be used very efficiently for the analysis of biomaterials. Before a new biomaterial is ready for implantation into tissues and widespread use in clinical practice, it must be extensively tested. Experimental cell models, which are a suitable testing ground and the first line of empirical exploration of new biomaterials, must contain suitable cells that form the basis of biomaterial testing. To isolate a stable and suitable cell culture, many steps are required. The first and one of the most important steps is the collection of donor tissue, usually during a surgical procedure. Thus, the collection is the foundation for the success of cell isolation. This article explains the sources and neurosurgical procedures for obtaining brain tissue samples for cell isolation techniques, which are essential for biomaterial testing procedures.
format	article
author	Lidija Gradišnik Roman Bošnjak Gorazd Bunc Janez Ravnik Tina Maver Tomaž Velnar
author_facet	Lidija Gradišnik Roman Bošnjak Gorazd Bunc Janez Ravnik Tina Maver Tomaž Velnar
author_sort	Lidija Gradišnik
title	Neurosurgical Approaches to Brain Tissue Harvesting for the Establishment of Cell Cultures in Neural Experimental Cell Models
title_short	Neurosurgical Approaches to Brain Tissue Harvesting for the Establishment of Cell Cultures in Neural Experimental Cell Models
title_full	Neurosurgical Approaches to Brain Tissue Harvesting for the Establishment of Cell Cultures in Neural Experimental Cell Models
title_fullStr	Neurosurgical Approaches to Brain Tissue Harvesting for the Establishment of Cell Cultures in Neural Experimental Cell Models
title_full_unstemmed	Neurosurgical Approaches to Brain Tissue Harvesting for the Establishment of Cell Cultures in Neural Experimental Cell Models
title_sort	neurosurgical approaches to brain tissue harvesting for the establishment of cell cultures in neural experimental cell models
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/b8e81d68b99a47c78766142815129b4a
work_keys_str_mv	AT lidijagradisnik neurosurgicalapproachestobraintissueharvestingfortheestablishmentofcellculturesinneuralexperimentalcellmodels AT romanbosnjak neurosurgicalapproachestobraintissueharvestingfortheestablishmentofcellculturesinneuralexperimentalcellmodels AT gorazdbunc neurosurgicalapproachestobraintissueharvestingfortheestablishmentofcellculturesinneuralexperimentalcellmodels AT janezravnik neurosurgicalapproachestobraintissueharvestingfortheestablishmentofcellculturesinneuralexperimentalcellmodels AT tinamaver neurosurgicalapproachestobraintissueharvestingfortheestablishmentofcellculturesinneuralexperimentalcellmodels AT tomazvelnar neurosurgicalapproachestobraintissueharvestingfortheestablishmentofcellculturesinneuralexperimentalcellmodels
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Neurosurgical Approaches to Brain Tissue Harvesting for the Establishment of Cell Cultures in Neural Experimental Cell Models

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