APPROACHES TO STANDARDIZATION OF THE FLOW CYTOMETRY METHOD FOR CELLS IMMUNOPHENOTIPING. CYTOMETRS ADJUSTMENT AND PREPARATION OF PROTOCOLS FOR ANALYSIS

Abstract. Immunophenotyping by using a flow cytometry method allows characterizing cells with the help monoclonal antibodies or any other probes and enables to judge their type and a functional condition on presence of this or that set of cellular markers and processes occurring in them. However as...

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Autor principal:	S. V. Khaidukov
Formato:	article
Lenguaje:	RU
Publicado:	SPb RAACI 2014
Materias:	flow cytometry discriminator indemnification multivariable analysis Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/b8ecffc7eed943ad9dac62b678022b59
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spelling	oai:doaj.org-article:b8ecffc7eed943ad9dac62b678022b592021-11-18T08:03:39ZAPPROACHES TO STANDARDIZATION OF THE FLOW CYTOMETRY METHOD FOR CELLS IMMUNOPHENOTIPING. CYTOMETRS ADJUSTMENT AND PREPARATION OF PROTOCOLS FOR ANALYSIS1563-06252313-741X10.15789/1563-0625-2007-6-569-574https://doaj.org/article/b8ecffc7eed943ad9dac62b678022b592014-07-01T00:00:00Zhttps://www.mimmun.ru/mimmun/article/view/242https://doaj.org/toc/1563-0625https://doaj.org/toc/2313-741XAbstract. Immunophenotyping by using a flow cytometry method allows characterizing cells with the help monoclonal antibodies or any other probes and enables to judge their type and a functional condition on presence of this or that set of cellular markers and processes occurring in them. However as a result of carrying out cytometryc analysis could be a number of mistakes at different stages of preparation cytometer to work and that leads to not correct result. To avoid it, the algorithm of preparation of reports for the analysis of cells of peripheral blood is offered. This algorithm of adjustment of the working report looks as follows: check of cytometers work; adjustment of the discriminator; adjustment of sensitivity of channels of light scatters; sensitivity adjustment of channels of fluorescence; introduction of factors of indemnification. Standard adjustments flow cytometers and reports will allow carrying out more correctly the analysis as in clinical diagnostic, and the research purposes.S. V. KhaidukovSPb RAACIarticleflow cytometrydiscriminatorindemnificationmultivariable analysisImmunologic diseases. AllergyRC581-607RUMedicinskaâ Immunologiâ, Vol 9, Iss 6, Pp 569-574 (2014)
institution	DOAJ
collection	DOAJ
language	RU
topic	flow cytometry discriminator indemnification multivariable analysis Immunologic diseases. Allergy RC581-607
spellingShingle	flow cytometry discriminator indemnification multivariable analysis Immunologic diseases. Allergy RC581-607 S. V. Khaidukov APPROACHES TO STANDARDIZATION OF THE FLOW CYTOMETRY METHOD FOR CELLS IMMUNOPHENOTIPING. CYTOMETRS ADJUSTMENT AND PREPARATION OF PROTOCOLS FOR ANALYSIS
description	Abstract. Immunophenotyping by using a flow cytometry method allows characterizing cells with the help monoclonal antibodies or any other probes and enables to judge their type and a functional condition on presence of this or that set of cellular markers and processes occurring in them. However as a result of carrying out cytometryc analysis could be a number of mistakes at different stages of preparation cytometer to work and that leads to not correct result. To avoid it, the algorithm of preparation of reports for the analysis of cells of peripheral blood is offered. This algorithm of adjustment of the working report looks as follows: check of cytometers work; adjustment of the discriminator; adjustment of sensitivity of channels of light scatters; sensitivity adjustment of channels of fluorescence; introduction of factors of indemnification. Standard adjustments flow cytometers and reports will allow carrying out more correctly the analysis as in clinical diagnostic, and the research purposes.
format	article
author	S. V. Khaidukov
author_facet	S. V. Khaidukov
author_sort	S. V. Khaidukov
title	APPROACHES TO STANDARDIZATION OF THE FLOW CYTOMETRY METHOD FOR CELLS IMMUNOPHENOTIPING. CYTOMETRS ADJUSTMENT AND PREPARATION OF PROTOCOLS FOR ANALYSIS
title_short	APPROACHES TO STANDARDIZATION OF THE FLOW CYTOMETRY METHOD FOR CELLS IMMUNOPHENOTIPING. CYTOMETRS ADJUSTMENT AND PREPARATION OF PROTOCOLS FOR ANALYSIS
title_full	APPROACHES TO STANDARDIZATION OF THE FLOW CYTOMETRY METHOD FOR CELLS IMMUNOPHENOTIPING. CYTOMETRS ADJUSTMENT AND PREPARATION OF PROTOCOLS FOR ANALYSIS
title_fullStr	APPROACHES TO STANDARDIZATION OF THE FLOW CYTOMETRY METHOD FOR CELLS IMMUNOPHENOTIPING. CYTOMETRS ADJUSTMENT AND PREPARATION OF PROTOCOLS FOR ANALYSIS
title_full_unstemmed	APPROACHES TO STANDARDIZATION OF THE FLOW CYTOMETRY METHOD FOR CELLS IMMUNOPHENOTIPING. CYTOMETRS ADJUSTMENT AND PREPARATION OF PROTOCOLS FOR ANALYSIS
title_sort	approaches to standardization of the flow cytometry method for cells immunophenotiping. cytometrs adjustment and preparation of protocols for analysis
publisher	SPb RAACI
publishDate	2014
url	https://doaj.org/article/b8ecffc7eed943ad9dac62b678022b59
work_keys_str_mv	AT svkhaidukov approachestostandardizationoftheflowcytometrymethodforcellsimmunophenotipingcytometrsadjustmentandpreparationofprotocolsforanalysis
_version_	1718422564377722880

APPROACHES TO STANDARDIZATION OF THE FLOW CYTOMETRY METHOD FOR CELLS IMMUNOPHENOTIPING. CYTOMETRS ADJUSTMENT AND PREPARATION OF PROTOCOLS FOR ANALYSIS

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