Whole blood transfusion improves vascular integrity and increases survival in artemether-treated experimental cerebral malaria

Abstract Pathological features observed in both human and experimental cerebral malaria (ECM) are endothelial dysfunction and changes in blood components. Blood transfusion has been routinely used in patients with severe malarial anemia and can also benefit comatose and acidotic malaria patients. In...

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Autores principales:	Saba Gul, Flavia L. Ribeiro-Gomes, Aline S. Moreira, Guilherme S. Sanches, Fabiana G. Conceição, Cláudio Tadeu Daniel-Ribeiro, Hans C. Ackerman, Leonardo J. M. Carvalho
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b8ef0055519d46e8a9563dd4cc95860d
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spelling	oai:doaj.org-article:b8ef0055519d46e8a9563dd4cc95860d2021-12-02T17:52:13ZWhole blood transfusion improves vascular integrity and increases survival in artemether-treated experimental cerebral malaria10.1038/s41598-021-91499-32045-2322https://doaj.org/article/b8ef0055519d46e8a9563dd4cc95860d2021-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-91499-3https://doaj.org/toc/2045-2322Abstract Pathological features observed in both human and experimental cerebral malaria (ECM) are endothelial dysfunction and changes in blood components. Blood transfusion has been routinely used in patients with severe malarial anemia and can also benefit comatose and acidotic malaria patients. In the present study Plasmodium berghei-infected mice were transfused intraperitoneally with 200 μL of whole blood along with 20 mg/kg of artemether. ECM mice showed severe thrombocytopenia and decreases in hematocrit. Artemether treatment markedly aggravated anemia within 24 h. Whole blood administration significantly prevented further drop in hematocrit and partially restored the platelet count. Increased levels of plasma angiopoietin-2 (Ang-2) remained high 24 h after artemether treatment but returned to normal levels 24 h after blood transfusion, indicating reversal to quiescence. Ang-1 was depleted in ECM mice and levels were not restored by any treatment. Blood transfusion prevented the aggravation of the breakdown of blood brain barrier after artemether treatment and decreased spleen congestion without affecting splenic lymphocyte populations. Critically, blood transfusion resulted in markedly improved survival of mice with ECM (75.9% compared to 50.9% receiving artemether only). These findings indicate that whole blood transfusion can be an effective adjuvant therapy for cerebral malaria.Saba GulFlavia L. Ribeiro-GomesAline S. MoreiraGuilherme S. SanchesFabiana G. ConceiçãoCláudio Tadeu Daniel-RibeiroHans C. AckermanLeonardo J. M. CarvalhoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Saba Gul Flavia L. Ribeiro-Gomes Aline S. Moreira Guilherme S. Sanches Fabiana G. Conceição Cláudio Tadeu Daniel-Ribeiro Hans C. Ackerman Leonardo J. M. Carvalho Whole blood transfusion improves vascular integrity and increases survival in artemether-treated experimental cerebral malaria
description	Abstract Pathological features observed in both human and experimental cerebral malaria (ECM) are endothelial dysfunction and changes in blood components. Blood transfusion has been routinely used in patients with severe malarial anemia and can also benefit comatose and acidotic malaria patients. In the present study Plasmodium berghei-infected mice were transfused intraperitoneally with 200 μL of whole blood along with 20 mg/kg of artemether. ECM mice showed severe thrombocytopenia and decreases in hematocrit. Artemether treatment markedly aggravated anemia within 24 h. Whole blood administration significantly prevented further drop in hematocrit and partially restored the platelet count. Increased levels of plasma angiopoietin-2 (Ang-2) remained high 24 h after artemether treatment but returned to normal levels 24 h after blood transfusion, indicating reversal to quiescence. Ang-1 was depleted in ECM mice and levels were not restored by any treatment. Blood transfusion prevented the aggravation of the breakdown of blood brain barrier after artemether treatment and decreased spleen congestion without affecting splenic lymphocyte populations. Critically, blood transfusion resulted in markedly improved survival of mice with ECM (75.9% compared to 50.9% receiving artemether only). These findings indicate that whole blood transfusion can be an effective adjuvant therapy for cerebral malaria.
format	article
author	Saba Gul Flavia L. Ribeiro-Gomes Aline S. Moreira Guilherme S. Sanches Fabiana G. Conceição Cláudio Tadeu Daniel-Ribeiro Hans C. Ackerman Leonardo J. M. Carvalho
author_facet	Saba Gul Flavia L. Ribeiro-Gomes Aline S. Moreira Guilherme S. Sanches Fabiana G. Conceição Cláudio Tadeu Daniel-Ribeiro Hans C. Ackerman Leonardo J. M. Carvalho
author_sort	Saba Gul
title	Whole blood transfusion improves vascular integrity and increases survival in artemether-treated experimental cerebral malaria
title_short	Whole blood transfusion improves vascular integrity and increases survival in artemether-treated experimental cerebral malaria
title_full	Whole blood transfusion improves vascular integrity and increases survival in artemether-treated experimental cerebral malaria
title_fullStr	Whole blood transfusion improves vascular integrity and increases survival in artemether-treated experimental cerebral malaria
title_full_unstemmed	Whole blood transfusion improves vascular integrity and increases survival in artemether-treated experimental cerebral malaria
title_sort	whole blood transfusion improves vascular integrity and increases survival in artemether-treated experimental cerebral malaria
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/b8ef0055519d46e8a9563dd4cc95860d
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Whole blood transfusion improves vascular integrity and increases survival in artemether-treated experimental cerebral malaria

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