Potential of ayurgenomics approach in complex trait research: leads from a pilot study on rheumatoid arthritis.

<h4>Background</h4>Inconsistent results across association studies including Genome-wide association, have posed a major challenge in complex disease genetics. Of the several factors which contribute to this, phenotypic heterogeneity is a serious limitation encountered in modern medicine...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Ramesh C Juyal, Sapna Negi, Preeti Wakhode, Sulekha Bhat, Bheema Bhat, B K Thelma
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b8f4f36f059649e890a09748e138e590
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:b8f4f36f059649e890a09748e138e590
record_format	dspace
spelling	oai:doaj.org-article:b8f4f36f059649e890a09748e138e5902021-11-18T08:13:54ZPotential of ayurgenomics approach in complex trait research: leads from a pilot study on rheumatoid arthritis.1932-620310.1371/journal.pone.0045752https://doaj.org/article/b8f4f36f059649e890a09748e138e5902012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23049851/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Inconsistent results across association studies including Genome-wide association, have posed a major challenge in complex disease genetics. Of the several factors which contribute to this, phenotypic heterogeneity is a serious limitation encountered in modern medicine. On the other hand, Ayurveda, a holistic Indian traditional system of medicine, enables subgrouping of individuals into three major categories namely Vata, Pitta and Kapha, based on their physical and mental constitution, referred to as Prakriti. We hypothesised that conditioning association studies on prior risk, predictable in Ayurveda, will uncover much more variance and potentially open up more predictive health.<h4>Objectives and methods</h4>Identification of genetic susceptibility markers by combining the prakriti based subgrouping of individuals with genetic analysis tools was attempted in a Rheumatoid arthritis (RA) cohort. Association of 21 markers from commonly implicated inflammatory and oxidative stress pathways was tested using a case-control approach in a total cohort comprising 325 cases and 356 controls and in the three subgroups separately. We also tested few postulates of Ayurveda on the disease characteristics in different prakriti groups using clinico-genetic data.<h4>Results</h4>Inflammatory genes like IL1β (C-C-C haplotype, p=0.0005, OR=3.09) and CD40 (rs4810485 allelic, p=0.04, OR=2.27) seem to be the determinants in Vata subgroup whereas oxidative stress pathway genes are observed in Pitta (SOD3 rs699473, p=0.004, OR=1.83; rs2536512 p=0.005; OR=1.88 and PON1 rs662, p=0.04, OR=1.53) and Kapha (SOD3 rs2536512, genotypic, p=0.02, OR=2.39) subgroups. Fixed effect analysis of the associated markers from CD40, SOD3 and TNFα with genotype X prakriti interaction terms suggests heterogeneity of effects within the subgroups. Further, disease characteristics such as severity was most pronounced in Vata group.<h4>Conclusions</h4>This exploratory study suggests discrete causal pathways for RA etiology in prakriti based subgroups, thereby, validating concepts of prakriti and personalized medicine in Ayurveda. Ayurgenomics approach holds promise for biomarker discovery in complex diseases.Ramesh C JuyalSapna NegiPreeti WakhodeSulekha BhatBheema BhatB K ThelmaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 9, p e45752 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ramesh C Juyal Sapna Negi Preeti Wakhode Sulekha Bhat Bheema Bhat B K Thelma Potential of ayurgenomics approach in complex trait research: leads from a pilot study on rheumatoid arthritis.
description	<h4>Background</h4>Inconsistent results across association studies including Genome-wide association, have posed a major challenge in complex disease genetics. Of the several factors which contribute to this, phenotypic heterogeneity is a serious limitation encountered in modern medicine. On the other hand, Ayurveda, a holistic Indian traditional system of medicine, enables subgrouping of individuals into three major categories namely Vata, Pitta and Kapha, based on their physical and mental constitution, referred to as Prakriti. We hypothesised that conditioning association studies on prior risk, predictable in Ayurveda, will uncover much more variance and potentially open up more predictive health.<h4>Objectives and methods</h4>Identification of genetic susceptibility markers by combining the prakriti based subgrouping of individuals with genetic analysis tools was attempted in a Rheumatoid arthritis (RA) cohort. Association of 21 markers from commonly implicated inflammatory and oxidative stress pathways was tested using a case-control approach in a total cohort comprising 325 cases and 356 controls and in the three subgroups separately. We also tested few postulates of Ayurveda on the disease characteristics in different prakriti groups using clinico-genetic data.<h4>Results</h4>Inflammatory genes like IL1β (C-C-C haplotype, p=0.0005, OR=3.09) and CD40 (rs4810485 allelic, p=0.04, OR=2.27) seem to be the determinants in Vata subgroup whereas oxidative stress pathway genes are observed in Pitta (SOD3 rs699473, p=0.004, OR=1.83; rs2536512 p=0.005; OR=1.88 and PON1 rs662, p=0.04, OR=1.53) and Kapha (SOD3 rs2536512, genotypic, p=0.02, OR=2.39) subgroups. Fixed effect analysis of the associated markers from CD40, SOD3 and TNFα with genotype X prakriti interaction terms suggests heterogeneity of effects within the subgroups. Further, disease characteristics such as severity was most pronounced in Vata group.<h4>Conclusions</h4>This exploratory study suggests discrete causal pathways for RA etiology in prakriti based subgroups, thereby, validating concepts of prakriti and personalized medicine in Ayurveda. Ayurgenomics approach holds promise for biomarker discovery in complex diseases.
format	article
author	Ramesh C Juyal Sapna Negi Preeti Wakhode Sulekha Bhat Bheema Bhat B K Thelma
author_facet	Ramesh C Juyal Sapna Negi Preeti Wakhode Sulekha Bhat Bheema Bhat B K Thelma
author_sort	Ramesh C Juyal
title	Potential of ayurgenomics approach in complex trait research: leads from a pilot study on rheumatoid arthritis.
title_short	Potential of ayurgenomics approach in complex trait research: leads from a pilot study on rheumatoid arthritis.
title_full	Potential of ayurgenomics approach in complex trait research: leads from a pilot study on rheumatoid arthritis.
title_fullStr	Potential of ayurgenomics approach in complex trait research: leads from a pilot study on rheumatoid arthritis.
title_full_unstemmed	Potential of ayurgenomics approach in complex trait research: leads from a pilot study on rheumatoid arthritis.
title_sort	potential of ayurgenomics approach in complex trait research: leads from a pilot study on rheumatoid arthritis.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/b8f4f36f059649e890a09748e138e590
work_keys_str_mv	AT rameshcjuyal potentialofayurgenomicsapproachincomplextraitresearchleadsfromapilotstudyonrheumatoidarthritis AT sapnanegi potentialofayurgenomicsapproachincomplextraitresearchleadsfromapilotstudyonrheumatoidarthritis AT preetiwakhode potentialofayurgenomicsapproachincomplextraitresearchleadsfromapilotstudyonrheumatoidarthritis AT sulekhabhat potentialofayurgenomicsapproachincomplextraitresearchleadsfromapilotstudyonrheumatoidarthritis AT bheemabhat potentialofayurgenomicsapproachincomplextraitresearchleadsfromapilotstudyonrheumatoidarthritis AT bkthelma potentialofayurgenomicsapproachincomplextraitresearchleadsfromapilotstudyonrheumatoidarthritis
_version_	1718422066005278720

Potential of ayurgenomics approach in complex trait research: leads from a pilot study on rheumatoid arthritis.

Ejemplares similares