Transcriptional hallmarks of cancer cell lines reveal an emerging role of branched chain amino acid catabolism

Abstract A comparative analysis between cancer cell lines and healthy dividing cells was performed using data (289 microarrays and 50 RNA-seq samples) from 100 different cancer cell lines and 6 types of healthy stem cells. The analysis revealed two large-scale transcriptional events that characteriz...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Ieva Antanavičiūtė, Valeryia Mikalayeva, Ieva Ceslevičienė, Gintarė Milašiūtė, Vytenis Arvydas Skeberdis, Sergio Bordel
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2017
Materias:
R
Q
Acceso en línea:https://doaj.org/article/b8fa77e54d9e4df0818a3bab0a846c01
Etiquetas: Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
id oai:doaj.org-article:b8fa77e54d9e4df0818a3bab0a846c01
record_format dspace
spelling oai:doaj.org-article:b8fa77e54d9e4df0818a3bab0a846c012021-12-02T12:30:45ZTranscriptional hallmarks of cancer cell lines reveal an emerging role of branched chain amino acid catabolism10.1038/s41598-017-08329-82045-2322https://doaj.org/article/b8fa77e54d9e4df0818a3bab0a846c012017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-08329-8https://doaj.org/toc/2045-2322Abstract A comparative analysis between cancer cell lines and healthy dividing cells was performed using data (289 microarrays and 50 RNA-seq samples) from 100 different cancer cell lines and 6 types of healthy stem cells. The analysis revealed two large-scale transcriptional events that characterize cancer cell lines. The first event was a large-scale up-regulation pattern associated to epithelial-mesenchymal transition, putatively driven by the interplay of the SP1 transcription factor and the canonical Wnt signaling pathway; the second event was the failure to overexpress a diverse set of genes coding membrane and extracellular proteins. This failure is putatively caused by a lack of activity of the AP-1 complex. It was also shown that the epithelial-mesenchymal transition was associated with the up-regulation of 5 enzymes involved in the degradation of branched chain amino acids. The suitability of silencing one of this enzymes (branched chain amino acid transaminase 2; BCAT2) with therapeutic effects was tested experimentally on the breast cancer cell line MCF-7 and primary cell culture of breast tumor (BCC), leading to lower cell proliferation. The silencing of BCAT2 did not have any significant effect on ASM and MCF10A cells, which were used as models of healthy dividing cells.Ieva AntanavičiūtėValeryia MikalayevaIeva CeslevičienėGintarė MilašiūtėVytenis Arvydas SkeberdisSergio BordelNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-12 (2017)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Ieva Antanavičiūtė
Valeryia Mikalayeva
Ieva Ceslevičienė
Gintarė Milašiūtė
Vytenis Arvydas Skeberdis
Sergio Bordel
Transcriptional hallmarks of cancer cell lines reveal an emerging role of branched chain amino acid catabolism
description Abstract A comparative analysis between cancer cell lines and healthy dividing cells was performed using data (289 microarrays and 50 RNA-seq samples) from 100 different cancer cell lines and 6 types of healthy stem cells. The analysis revealed two large-scale transcriptional events that characterize cancer cell lines. The first event was a large-scale up-regulation pattern associated to epithelial-mesenchymal transition, putatively driven by the interplay of the SP1 transcription factor and the canonical Wnt signaling pathway; the second event was the failure to overexpress a diverse set of genes coding membrane and extracellular proteins. This failure is putatively caused by a lack of activity of the AP-1 complex. It was also shown that the epithelial-mesenchymal transition was associated with the up-regulation of 5 enzymes involved in the degradation of branched chain amino acids. The suitability of silencing one of this enzymes (branched chain amino acid transaminase 2; BCAT2) with therapeutic effects was tested experimentally on the breast cancer cell line MCF-7 and primary cell culture of breast tumor (BCC), leading to lower cell proliferation. The silencing of BCAT2 did not have any significant effect on ASM and MCF10A cells, which were used as models of healthy dividing cells.
format article
author Ieva Antanavičiūtė
Valeryia Mikalayeva
Ieva Ceslevičienė
Gintarė Milašiūtė
Vytenis Arvydas Skeberdis
Sergio Bordel
author_facet Ieva Antanavičiūtė
Valeryia Mikalayeva
Ieva Ceslevičienė
Gintarė Milašiūtė
Vytenis Arvydas Skeberdis
Sergio Bordel
author_sort Ieva Antanavičiūtė
title Transcriptional hallmarks of cancer cell lines reveal an emerging role of branched chain amino acid catabolism
title_short Transcriptional hallmarks of cancer cell lines reveal an emerging role of branched chain amino acid catabolism
title_full Transcriptional hallmarks of cancer cell lines reveal an emerging role of branched chain amino acid catabolism
title_fullStr Transcriptional hallmarks of cancer cell lines reveal an emerging role of branched chain amino acid catabolism
title_full_unstemmed Transcriptional hallmarks of cancer cell lines reveal an emerging role of branched chain amino acid catabolism
title_sort transcriptional hallmarks of cancer cell lines reveal an emerging role of branched chain amino acid catabolism
publisher Nature Portfolio
publishDate 2017
url https://doaj.org/article/b8fa77e54d9e4df0818a3bab0a846c01
work_keys_str_mv AT ievaantanaviciute transcriptionalhallmarksofcancercelllinesrevealanemergingroleofbranchedchainaminoacidcatabolism
AT valeryiamikalayeva transcriptionalhallmarksofcancercelllinesrevealanemergingroleofbranchedchainaminoacidcatabolism
AT ievacesleviciene transcriptionalhallmarksofcancercelllinesrevealanemergingroleofbranchedchainaminoacidcatabolism
AT gintaremilasiute transcriptionalhallmarksofcancercelllinesrevealanemergingroleofbranchedchainaminoacidcatabolism
AT vytenisarvydasskeberdis transcriptionalhallmarksofcancercelllinesrevealanemergingroleofbranchedchainaminoacidcatabolism
AT sergiobordel transcriptionalhallmarksofcancercelllinesrevealanemergingroleofbranchedchainaminoacidcatabolism
_version_ 1718394357169520640