Reduced CTGF expression promotes cell growth, migration, and invasion in nasopharyngeal carcinoma.

<h4>Background</h4>The role of CTGF varies in different types of cancer. The purpose of this study is to investigate the involvement of CTGF in tumor progression and prognosis of human nasopharyngeal carcinoma (NPC).<h4>Experimental design</h4>CTGF expression levels were exam...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Yan Zhen, Yanfen Ye, Xiaoli Yu, Chunping Mai, Ying Zhou, Yan Chen, Huiling Yang, Xiaoming Lyu, Ye Song, Qiangyun Wu, Qiaofen Fu, Mengyang Zhao, Shengni Hua, Hao Wang, Zhen Liu, Yajie Zhang, Weiyi Fang
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b8fff61713ba4587b39030e6016f3485
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:b8fff61713ba4587b39030e6016f3485
record_format	dspace
spelling	oai:doaj.org-article:b8fff61713ba4587b39030e6016f34852021-11-18T07:43:15ZReduced CTGF expression promotes cell growth, migration, and invasion in nasopharyngeal carcinoma.1932-620310.1371/journal.pone.0064976https://doaj.org/article/b8fff61713ba4587b39030e6016f34852014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23755163/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>The role of CTGF varies in different types of cancer. The purpose of this study is to investigate the involvement of CTGF in tumor progression and prognosis of human nasopharyngeal carcinoma (NPC).<h4>Experimental design</h4>CTGF expression levels were examined in NPC tissues and cells, nasopharynx (NP) tissues, and NP69 cells. The effects and molecular mechanisms of CTGF expression on cell proliferation, migration, invasion, and cell cycle were also explored.<h4>Results</h4>NPC cells exhibited decreased mRNA expression of CTGF compared to immortalized human nasopharyngeal epithelial cell line NP69. Similarly, CTGF was observed to be downregulated in NPC compared to normal tissues at mRNA and protein levels. Furthermore, reduced CTGF was negatively associated with the progression of NPC. Knocking down CTGF expression enhanced the colony formation, cell migration, invasion, and G1/S cell cycle transition. Mechanistic analysis revealed that CTGF suppression activated FAK/PI3K/AKT and its downstream signals regulating the cell cycle, epithelial-mesenchymal transition (EMT) and MMPs. Finally, DNA methylation microarray revealed a lack of hypermethylation at the CTGF promoter, suggesting other mechanisms are associated with suppression of CTGF in NPC.<h4>Conclusion</h4>Our study demonstrates that reduced expression of CTGF promoted cell proliferation, migration, invasion and cell cycle progression through FAK/PI3K/AKT, EMT and MMP pathways in NPC.Yan ZhenYanfen YeXiaoli YuChunping MaiYing ZhouYan ChenHuiling YangXiaoming LyuYe SongQiangyun WuQiaofen FuMengyang ZhaoShengni HuaHao WangZhen LiuYajie ZhangWeiyi FangPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 6, p e64976 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yan Zhen Yanfen Ye Xiaoli Yu Chunping Mai Ying Zhou Yan Chen Huiling Yang Xiaoming Lyu Ye Song Qiangyun Wu Qiaofen Fu Mengyang Zhao Shengni Hua Hao Wang Zhen Liu Yajie Zhang Weiyi Fang Reduced CTGF expression promotes cell growth, migration, and invasion in nasopharyngeal carcinoma.
description	<h4>Background</h4>The role of CTGF varies in different types of cancer. The purpose of this study is to investigate the involvement of CTGF in tumor progression and prognosis of human nasopharyngeal carcinoma (NPC).<h4>Experimental design</h4>CTGF expression levels were examined in NPC tissues and cells, nasopharynx (NP) tissues, and NP69 cells. The effects and molecular mechanisms of CTGF expression on cell proliferation, migration, invasion, and cell cycle were also explored.<h4>Results</h4>NPC cells exhibited decreased mRNA expression of CTGF compared to immortalized human nasopharyngeal epithelial cell line NP69. Similarly, CTGF was observed to be downregulated in NPC compared to normal tissues at mRNA and protein levels. Furthermore, reduced CTGF was negatively associated with the progression of NPC. Knocking down CTGF expression enhanced the colony formation, cell migration, invasion, and G1/S cell cycle transition. Mechanistic analysis revealed that CTGF suppression activated FAK/PI3K/AKT and its downstream signals regulating the cell cycle, epithelial-mesenchymal transition (EMT) and MMPs. Finally, DNA methylation microarray revealed a lack of hypermethylation at the CTGF promoter, suggesting other mechanisms are associated with suppression of CTGF in NPC.<h4>Conclusion</h4>Our study demonstrates that reduced expression of CTGF promoted cell proliferation, migration, invasion and cell cycle progression through FAK/PI3K/AKT, EMT and MMP pathways in NPC.
format	article
author	Yan Zhen Yanfen Ye Xiaoli Yu Chunping Mai Ying Zhou Yan Chen Huiling Yang Xiaoming Lyu Ye Song Qiangyun Wu Qiaofen Fu Mengyang Zhao Shengni Hua Hao Wang Zhen Liu Yajie Zhang Weiyi Fang
author_facet	Yan Zhen Yanfen Ye Xiaoli Yu Chunping Mai Ying Zhou Yan Chen Huiling Yang Xiaoming Lyu Ye Song Qiangyun Wu Qiaofen Fu Mengyang Zhao Shengni Hua Hao Wang Zhen Liu Yajie Zhang Weiyi Fang
author_sort	Yan Zhen
title	Reduced CTGF expression promotes cell growth, migration, and invasion in nasopharyngeal carcinoma.
title_short	Reduced CTGF expression promotes cell growth, migration, and invasion in nasopharyngeal carcinoma.
title_full	Reduced CTGF expression promotes cell growth, migration, and invasion in nasopharyngeal carcinoma.
title_fullStr	Reduced CTGF expression promotes cell growth, migration, and invasion in nasopharyngeal carcinoma.
title_full_unstemmed	Reduced CTGF expression promotes cell growth, migration, and invasion in nasopharyngeal carcinoma.
title_sort	reduced ctgf expression promotes cell growth, migration, and invasion in nasopharyngeal carcinoma.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/b8fff61713ba4587b39030e6016f3485
work_keys_str_mv	AT yanzhen reducedctgfexpressionpromotescellgrowthmigrationandinvasioninnasopharyngealcarcinoma AT yanfenye reducedctgfexpressionpromotescellgrowthmigrationandinvasioninnasopharyngealcarcinoma AT xiaoliyu reducedctgfexpressionpromotescellgrowthmigrationandinvasioninnasopharyngealcarcinoma AT chunpingmai reducedctgfexpressionpromotescellgrowthmigrationandinvasioninnasopharyngealcarcinoma AT yingzhou reducedctgfexpressionpromotescellgrowthmigrationandinvasioninnasopharyngealcarcinoma AT yanchen reducedctgfexpressionpromotescellgrowthmigrationandinvasioninnasopharyngealcarcinoma AT huilingyang reducedctgfexpressionpromotescellgrowthmigrationandinvasioninnasopharyngealcarcinoma AT xiaominglyu reducedctgfexpressionpromotescellgrowthmigrationandinvasioninnasopharyngealcarcinoma AT yesong reducedctgfexpressionpromotescellgrowthmigrationandinvasioninnasopharyngealcarcinoma AT qiangyunwu reducedctgfexpressionpromotescellgrowthmigrationandinvasioninnasopharyngealcarcinoma AT qiaofenfu reducedctgfexpressionpromotescellgrowthmigrationandinvasioninnasopharyngealcarcinoma AT mengyangzhao reducedctgfexpressionpromotescellgrowthmigrationandinvasioninnasopharyngealcarcinoma AT shengnihua reducedctgfexpressionpromotescellgrowthmigrationandinvasioninnasopharyngealcarcinoma AT haowang reducedctgfexpressionpromotescellgrowthmigrationandinvasioninnasopharyngealcarcinoma AT zhenliu reducedctgfexpressionpromotescellgrowthmigrationandinvasioninnasopharyngealcarcinoma AT yajiezhang reducedctgfexpressionpromotescellgrowthmigrationandinvasioninnasopharyngealcarcinoma AT weiyifang reducedctgfexpressionpromotescellgrowthmigrationandinvasioninnasopharyngealcarcinoma
_version_	1718423058087149568

Reduced CTGF expression promotes cell growth, migration, and invasion in nasopharyngeal carcinoma.

Ejemplares similares