Cryopreservation protocol for human biliary tree stem/progenitors, hepatic and pancreatic precursors

Abstract Human biliary tree stem/progenitor cells (hBTSCs) are being used for cell therapies of patients with liver cirrhosis. A cryopreservation method was established to optimize sourcing of hBTSCs for these clinical programs and that comprises serum-free Kubota’s Medium (KM) supplemented with 10%...

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Autores principales:	Lorenzo Nevi, Vincenzo Cardinale, Guido Carpino, Daniele Costantini, Sabina Di Matteo, Alfredo Cantafora, Fabio Melandro, Roberto Brunelli, Carlo Bastianelli, Camilla Aliberti, Marco Monti, Daniela Bosco, Pasquale Bartolomeo Berloco, Pierluigi Benedetti Panici, Lola Reid, Eugenio Gaudio, Domenico Alvaro
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b90fb1f259eb4becb8d0886c88ca6666
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spelling	oai:doaj.org-article:b90fb1f259eb4becb8d0886c88ca66662021-12-02T12:32:52ZCryopreservation protocol for human biliary tree stem/progenitors, hepatic and pancreatic precursors10.1038/s41598-017-05858-02045-2322https://doaj.org/article/b90fb1f259eb4becb8d0886c88ca66662017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-05858-0https://doaj.org/toc/2045-2322Abstract Human biliary tree stem/progenitor cells (hBTSCs) are being used for cell therapies of patients with liver cirrhosis. A cryopreservation method was established to optimize sourcing of hBTSCs for these clinical programs and that comprises serum-free Kubota’s Medium (KM) supplemented with 10% dimethyl sulfoxide (DMSO), 15% human serum albumin (HSA) and 0.1% hyaluronans. Cryopreserved versus freshly isolated hBTSCs were similar in vitro with respect to self-replication, stemness traits, and multipotency. They were able to differentiate to functional hepatocytes,cholangiocytes or pancreatic islets, yielding similar levels of secretion of albumin or of glucose-inducible levels of insulin. Cryopreserved versus freshly isolated hBTSCs were equally able to engraft into immunocompromised mice yielding cells with human-specific gene expression and human albumin levels in murine serum that were higher for cryopreserved than for freshly isolated hBTSCs. The successful cryopreservation of hBTSCs facilitates establishment of hBTSCs cell banking offering logistical advantages for clinical programs for treatment of liver diseases.Lorenzo NeviVincenzo CardinaleGuido CarpinoDaniele CostantiniSabina Di MatteoAlfredo CantaforaFabio MelandroRoberto BrunelliCarlo BastianelliCamilla AlibertiMarco MontiDaniela BoscoPasquale Bartolomeo BerlocoPierluigi Benedetti PaniciLola ReidEugenio GaudioDomenico AlvaroNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-15 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Lorenzo Nevi Vincenzo Cardinale Guido Carpino Daniele Costantini Sabina Di Matteo Alfredo Cantafora Fabio Melandro Roberto Brunelli Carlo Bastianelli Camilla Aliberti Marco Monti Daniela Bosco Pasquale Bartolomeo Berloco Pierluigi Benedetti Panici Lola Reid Eugenio Gaudio Domenico Alvaro Cryopreservation protocol for human biliary tree stem/progenitors, hepatic and pancreatic precursors
description	Abstract Human biliary tree stem/progenitor cells (hBTSCs) are being used for cell therapies of patients with liver cirrhosis. A cryopreservation method was established to optimize sourcing of hBTSCs for these clinical programs and that comprises serum-free Kubota’s Medium (KM) supplemented with 10% dimethyl sulfoxide (DMSO), 15% human serum albumin (HSA) and 0.1% hyaluronans. Cryopreserved versus freshly isolated hBTSCs were similar in vitro with respect to self-replication, stemness traits, and multipotency. They were able to differentiate to functional hepatocytes,cholangiocytes or pancreatic islets, yielding similar levels of secretion of albumin or of glucose-inducible levels of insulin. Cryopreserved versus freshly isolated hBTSCs were equally able to engraft into immunocompromised mice yielding cells with human-specific gene expression and human albumin levels in murine serum that were higher for cryopreserved than for freshly isolated hBTSCs. The successful cryopreservation of hBTSCs facilitates establishment of hBTSCs cell banking offering logistical advantages for clinical programs for treatment of liver diseases.
format	article
author	Lorenzo Nevi Vincenzo Cardinale Guido Carpino Daniele Costantini Sabina Di Matteo Alfredo Cantafora Fabio Melandro Roberto Brunelli Carlo Bastianelli Camilla Aliberti Marco Monti Daniela Bosco Pasquale Bartolomeo Berloco Pierluigi Benedetti Panici Lola Reid Eugenio Gaudio Domenico Alvaro
author_facet	Lorenzo Nevi Vincenzo Cardinale Guido Carpino Daniele Costantini Sabina Di Matteo Alfredo Cantafora Fabio Melandro Roberto Brunelli Carlo Bastianelli Camilla Aliberti Marco Monti Daniela Bosco Pasquale Bartolomeo Berloco Pierluigi Benedetti Panici Lola Reid Eugenio Gaudio Domenico Alvaro
author_sort	Lorenzo Nevi
title	Cryopreservation protocol for human biliary tree stem/progenitors, hepatic and pancreatic precursors
title_short	Cryopreservation protocol for human biliary tree stem/progenitors, hepatic and pancreatic precursors
title_full	Cryopreservation protocol for human biliary tree stem/progenitors, hepatic and pancreatic precursors
title_fullStr	Cryopreservation protocol for human biliary tree stem/progenitors, hepatic and pancreatic precursors
title_full_unstemmed	Cryopreservation protocol for human biliary tree stem/progenitors, hepatic and pancreatic precursors
title_sort	cryopreservation protocol for human biliary tree stem/progenitors, hepatic and pancreatic precursors
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/b90fb1f259eb4becb8d0886c88ca6666
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Cryopreservation protocol for human biliary tree stem/progenitors, hepatic and pancreatic precursors

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