In vivo continuous evolution of genes and pathways in yeast

Directed evolution is a powerful technique for generating improved biological systems through repeated rounds of mutagenesis and selection. Here the authors engineer the yeast retrotransposon Ty1 to enable the creation of large mutant libraries in vivoand use this system to generate improved variant...

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Autores principales:	Nathan Crook, Joseph Abatemarco, Jie Sun, James M. Wagner, Alexander Schmitz, Hal S. Alper
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2016
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/b91259fab2d34416903a4dc5b5273dcd
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spelling	oai:doaj.org-article:b91259fab2d34416903a4dc5b5273dcd2021-12-02T17:33:09ZIn vivo continuous evolution of genes and pathways in yeast10.1038/ncomms130512041-1723https://doaj.org/article/b91259fab2d34416903a4dc5b5273dcd2016-10-01T00:00:00Zhttps://doi.org/10.1038/ncomms13051https://doaj.org/toc/2041-1723Directed evolution is a powerful technique for generating improved biological systems through repeated rounds of mutagenesis and selection. Here the authors engineer the yeast retrotransposon Ty1 to enable the creation of large mutant libraries in vivoand use this system to generate improved variants of single enzymes and multigene pathways.Nathan CrookJoseph AbatemarcoJie SunJames M. WagnerAlexander SchmitzHal S. AlperNature PortfolioarticleScienceQENNature Communications, Vol 7, Iss 1, Pp 1-14 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Nathan Crook Joseph Abatemarco Jie Sun James M. Wagner Alexander Schmitz Hal S. Alper In vivo continuous evolution of genes and pathways in yeast
description	Directed evolution is a powerful technique for generating improved biological systems through repeated rounds of mutagenesis and selection. Here the authors engineer the yeast retrotransposon Ty1 to enable the creation of large mutant libraries in vivoand use this system to generate improved variants of single enzymes and multigene pathways.
format	article
author	Nathan Crook Joseph Abatemarco Jie Sun James M. Wagner Alexander Schmitz Hal S. Alper
author_facet	Nathan Crook Joseph Abatemarco Jie Sun James M. Wagner Alexander Schmitz Hal S. Alper
author_sort	Nathan Crook
title	In vivo continuous evolution of genes and pathways in yeast
title_short	In vivo continuous evolution of genes and pathways in yeast
title_full	In vivo continuous evolution of genes and pathways in yeast
title_fullStr	In vivo continuous evolution of genes and pathways in yeast
title_full_unstemmed	In vivo continuous evolution of genes and pathways in yeast
title_sort	in vivo continuous evolution of genes and pathways in yeast
publisher	Nature Portfolio
publishDate	2016
url	https://doaj.org/article/b91259fab2d34416903a4dc5b5273dcd
work_keys_str_mv	AT nathancrook invivocontinuousevolutionofgenesandpathwaysinyeast AT josephabatemarco invivocontinuousevolutionofgenesandpathwaysinyeast AT jiesun invivocontinuousevolutionofgenesandpathwaysinyeast AT jamesmwagner invivocontinuousevolutionofgenesandpathwaysinyeast AT alexanderschmitz invivocontinuousevolutionofgenesandpathwaysinyeast AT halsalper invivocontinuousevolutionofgenesandpathwaysinyeast
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In vivo continuous evolution of genes and pathways in yeast

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