In vivo continuous evolution of genes and pathways in yeast

Directed evolution is a powerful technique for generating improved biological systems through repeated rounds of mutagenesis and selection. Here the authors engineer the yeast retrotransposon Ty1 to enable the creation of large mutant libraries in vivoand use this system to generate improved variant...

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Autores principales: Nathan Crook, Joseph Abatemarco, Jie Sun, James M. Wagner, Alexander Schmitz, Hal S. Alper
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2016
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Acceso en línea:https://doaj.org/article/b91259fab2d34416903a4dc5b5273dcd
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