Hyperbaric oxygen therapy attenuates neuronal apoptosis induced by traumatic brain injury via Akt/GSK3β/β-catenin pathway

Hyperbaric oxygen therapy attenuates neuronal apoptosis induced by traumatic brain injury via Akt/GSK3β/β-catenin pathway

Hui He,1 Xiufang Li,2 Yuling He1 1Department of Emergency, Zhuji People’s Hospital of Zhejiang Province, Zhuji, Zhejiang, China; 2Department of Pathology, Zhuji People’s Hospital of Zhejiang Province, Zhuji, Zhejiang, China Background: Given that the therapeutic effect of hyper...

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Autores principales: He H, Li X, He Y
Formato: article
Lenguaje:EN
Publicado: Dove Medical Press 2019
Materias:
Hyperbaric Oxygen
TBI
apoptosis
Akt
GSK3β
β-catenin
Neurosciences. Biological psychiatry. Neuropsychiatry
RC321-571
Neurology. Diseases of the nervous system
RC346-429
Acceso en línea:https://doaj.org/article/b914f720344340d8890eb734ed4e0215
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spelling oai:doaj.org-article:b914f720344340d8890eb734ed4e02152021-12-02T02:37:23ZHyperbaric oxygen therapy attenuates neuronal apoptosis induced by traumatic brain injury via Akt/GSK3β/β-catenin pathway1178-2021https://doaj.org/article/b914f720344340d8890eb734ed4e02152019-01-01T00:00:00Zhttps://www.dovepress.com/hyperbaric-oxygen-therapy-attenuates-neuronal-apoptosis-induced-by-tra-peer-reviewed-article-NDThttps://doaj.org/toc/1178-2021Hui He,1 Xiufang Li,2 Yuling He1 1Department of Emergency, Zhuji People’s Hospital of Zhejiang Province, Zhuji, Zhejiang, China; 2Department of Pathology, Zhuji People’s Hospital of Zhejiang Province, Zhuji, Zhejiang, China Background: Given that the therapeutic effect of hyperbaric oxygen (HBO) therapy on traumatic brain injury (TBI) has been debated for a long time, it is necessary to clarify the mechanism underlying the effect of HBO on acute TBI.Methods: This study investigated the effect of HBO therapy on neuronal apoptosis induced by acute TBI using the mouse model of TBI. The number of apoptotic cells and expression of apoptosis-associated factors (including caspase 3, pAkt/Akt, pGSK3β/GSK3β, and β-catenin) in pericontusional cortices of mice exposed to sham, TBI, and TBI + HBO treatment were measured and analyzed using TUNEL assay, quantitative reverse-transcription PCR, and Western blot.Results: Results showed that acute TBI increased the number of apoptotic neurons and mRNA expression and activated caspase 3 protein. With regard to proteins, acute TBI also resulted in decreased levels of pAkt/Akt, pGSK3β/GSK3β, and β-catenin, which facilitates neuronal apoptosis. This study shows that HBO therapy reversed these changes of pAkt/Akt, pGSK3β/GSK3β, and β-catenin induced by acute TBI and attenuated the apoptotic process in the pericontusional cortex.Conclusion: This study demonstrates the beneficial effect of HBO therapy on neuronal apoptosis caused by acute TBI. Furthermore, the mechanism underlying the therapeutic effect of HBO on acute TBI partly involves the Akt/GSK3β/β-catenin pathway. Keywords: hyperbaric oxygen, TBI, apoptosis, Akt, GSK3β, β-catenin  He HLi XHe YDove Medical PressarticleHyperbaric OxygenTBIapoptosisAktGSK3ββ-cateninNeurosciences. Biological psychiatry. NeuropsychiatryRC321-571Neurology. Diseases of the nervous systemRC346-429ENNeuropsychiatric Disease and Treatment, Vol Volume 15, Pp 369-374 (2019)
institution DOAJ
collection DOAJ
language EN
topic Hyperbaric Oxygen
TBI
apoptosis
Akt
GSK3β
β-catenin
Neurosciences. Biological psychiatry. Neuropsychiatry
RC321-571
Neurology. Diseases of the nervous system
RC346-429
spellingShingle Hyperbaric Oxygen
TBI
apoptosis
Akt
GSK3β
β-catenin
Neurosciences. Biological psychiatry. Neuropsychiatry
RC321-571
Neurology. Diseases of the nervous system
RC346-429
He H
Li X
He Y
Hyperbaric oxygen therapy attenuates neuronal apoptosis induced by traumatic brain injury via Akt/GSK3β/β-catenin pathway
description Hui He,1 Xiufang Li,2 Yuling He1 1Department of Emergency, Zhuji People’s Hospital of Zhejiang Province, Zhuji, Zhejiang, China; 2Department of Pathology, Zhuji People’s Hospital of Zhejiang Province, Zhuji, Zhejiang, China Background: Given that the therapeutic effect of hyperbaric oxygen (HBO) therapy on traumatic brain injury (TBI) has been debated for a long time, it is necessary to clarify the mechanism underlying the effect of HBO on acute TBI.Methods: This study investigated the effect of HBO therapy on neuronal apoptosis induced by acute TBI using the mouse model of TBI. The number of apoptotic cells and expression of apoptosis-associated factors (including caspase 3, pAkt/Akt, pGSK3β/GSK3β, and β-catenin) in pericontusional cortices of mice exposed to sham, TBI, and TBI + HBO treatment were measured and analyzed using TUNEL assay, quantitative reverse-transcription PCR, and Western blot.Results: Results showed that acute TBI increased the number of apoptotic neurons and mRNA expression and activated caspase 3 protein. With regard to proteins, acute TBI also resulted in decreased levels of pAkt/Akt, pGSK3β/GSK3β, and β-catenin, which facilitates neuronal apoptosis. This study shows that HBO therapy reversed these changes of pAkt/Akt, pGSK3β/GSK3β, and β-catenin induced by acute TBI and attenuated the apoptotic process in the pericontusional cortex.Conclusion: This study demonstrates the beneficial effect of HBO therapy on neuronal apoptosis caused by acute TBI. Furthermore, the mechanism underlying the therapeutic effect of HBO on acute TBI partly involves the Akt/GSK3β/β-catenin pathway. Keywords: hyperbaric oxygen, TBI, apoptosis, Akt, GSK3β, β-catenin  
format article
author He H
Li X
He Y
author_facet He H
Li X
He Y
author_sort He H
title Hyperbaric oxygen therapy attenuates neuronal apoptosis induced by traumatic brain injury via Akt/GSK3β/β-catenin pathway
title_short Hyperbaric oxygen therapy attenuates neuronal apoptosis induced by traumatic brain injury via Akt/GSK3β/β-catenin pathway
title_full Hyperbaric oxygen therapy attenuates neuronal apoptosis induced by traumatic brain injury via Akt/GSK3β/β-catenin pathway
title_fullStr Hyperbaric oxygen therapy attenuates neuronal apoptosis induced by traumatic brain injury via Akt/GSK3β/β-catenin pathway
title_full_unstemmed Hyperbaric oxygen therapy attenuates neuronal apoptosis induced by traumatic brain injury via Akt/GSK3β/β-catenin pathway
title_sort hyperbaric oxygen therapy attenuates neuronal apoptosis induced by traumatic brain injury via akt/gsk3β/β-catenin pathway
publisher Dove Medical Press
publishDate 2019
url https://doaj.org/article/b914f720344340d8890eb734ed4e0215
work_keys_str_mv AT heh hyperbaricoxygentherapyattenuatesneuronalapoptosisinducedbytraumaticbraininjuryviaaktgsk3betabetacateninpathway
AT lix hyperbaricoxygentherapyattenuatesneuronalapoptosisinducedbytraumaticbraininjuryviaaktgsk3betabetacateninpathway
AT hey hyperbaricoxygentherapyattenuatesneuronalapoptosisinducedbytraumaticbraininjuryviaaktgsk3betabetacateninpathway
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