Urinary miRNA profiles discriminate between obstruction-induced bladder dysfunction and healthy controls

Abstract Urgency, frequency and incomplete emptying are the troublesome symptoms often shared between benign prostatic obstruction-induced (BLUTD) and neurogenic (NLUTD) lower urinary tract dysfunction. Previously, using bladder biopsies, we suggested a panel of miRNA biomarkers for different functi...

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Autores principales:	Michelle von Siebenthal, Mustafa Besic, Ali Hashemi Gheinani, Akshay Akshay, Salomé Lizun-Platoni, Nadine Kunz, Fiona C. Burkhard, Katia Monastyrskaya
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b9151e85cf784c6ca7945427e6c5fcef
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spelling	oai:doaj.org-article:b9151e85cf784c6ca7945427e6c5fcef2021-12-02T17:16:14ZUrinary miRNA profiles discriminate between obstruction-induced bladder dysfunction and healthy controls10.1038/s41598-021-89535-32045-2322https://doaj.org/article/b9151e85cf784c6ca7945427e6c5fcef2021-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-89535-3https://doaj.org/toc/2045-2322Abstract Urgency, frequency and incomplete emptying are the troublesome symptoms often shared between benign prostatic obstruction-induced (BLUTD) and neurogenic (NLUTD) lower urinary tract dysfunction. Previously, using bladder biopsies, we suggested a panel of miRNA biomarkers for different functional phenotypes of the bladder. Urine is a good source of circulating miRNAs, but sex- and age-matched controls are important for urinary metabolite comparison. In two groups of healthy subjects (average age 32 and 57 years old, respectively) the total protein and RNA content was very similar between age groups, but the number of secreted extracellular vesicles (uEVs) and expression of several miRNAs were higher in the young healthy male volunteers. Timing of urine collection was not important for these parameters. We also evaluated the suitability of urinary miRNAs for non-invasive diagnosis of bladder outlet obstruction (BOO). A three urinary miRNA signature (miR-10a-5p, miR-301b-3p and miR-363-3p) could discriminate between controls and patients with LUTD (BLUTD and NLUTD). This panel of representative miRNAs can be further explored to develop a non-invasive diagnostic test for BOO. The age-related discrepancy in the urinary miRNA content observed in this study points to the importance of selecting appropriate, age-matched controls.Michelle von SiebenthalMustafa BesicAli Hashemi GheinaniAkshay AkshaySalomé Lizun-PlatoniNadine KunzFiona C. BurkhardKatia MonastyrskayaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-15 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Michelle von Siebenthal Mustafa Besic Ali Hashemi Gheinani Akshay Akshay Salomé Lizun-Platoni Nadine Kunz Fiona C. Burkhard Katia Monastyrskaya Urinary miRNA profiles discriminate between obstruction-induced bladder dysfunction and healthy controls
description	Abstract Urgency, frequency and incomplete emptying are the troublesome symptoms often shared between benign prostatic obstruction-induced (BLUTD) and neurogenic (NLUTD) lower urinary tract dysfunction. Previously, using bladder biopsies, we suggested a panel of miRNA biomarkers for different functional phenotypes of the bladder. Urine is a good source of circulating miRNAs, but sex- and age-matched controls are important for urinary metabolite comparison. In two groups of healthy subjects (average age 32 and 57 years old, respectively) the total protein and RNA content was very similar between age groups, but the number of secreted extracellular vesicles (uEVs) and expression of several miRNAs were higher in the young healthy male volunteers. Timing of urine collection was not important for these parameters. We also evaluated the suitability of urinary miRNAs for non-invasive diagnosis of bladder outlet obstruction (BOO). A three urinary miRNA signature (miR-10a-5p, miR-301b-3p and miR-363-3p) could discriminate between controls and patients with LUTD (BLUTD and NLUTD). This panel of representative miRNAs can be further explored to develop a non-invasive diagnostic test for BOO. The age-related discrepancy in the urinary miRNA content observed in this study points to the importance of selecting appropriate, age-matched controls.
format	article
author	Michelle von Siebenthal Mustafa Besic Ali Hashemi Gheinani Akshay Akshay Salomé Lizun-Platoni Nadine Kunz Fiona C. Burkhard Katia Monastyrskaya
author_facet	Michelle von Siebenthal Mustafa Besic Ali Hashemi Gheinani Akshay Akshay Salomé Lizun-Platoni Nadine Kunz Fiona C. Burkhard Katia Monastyrskaya
author_sort	Michelle von Siebenthal
title	Urinary miRNA profiles discriminate between obstruction-induced bladder dysfunction and healthy controls
title_short	Urinary miRNA profiles discriminate between obstruction-induced bladder dysfunction and healthy controls
title_full	Urinary miRNA profiles discriminate between obstruction-induced bladder dysfunction and healthy controls
title_fullStr	Urinary miRNA profiles discriminate between obstruction-induced bladder dysfunction and healthy controls
title_full_unstemmed	Urinary miRNA profiles discriminate between obstruction-induced bladder dysfunction and healthy controls
title_sort	urinary mirna profiles discriminate between obstruction-induced bladder dysfunction and healthy controls
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/b9151e85cf784c6ca7945427e6c5fcef
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Urinary miRNA profiles discriminate between obstruction-induced bladder dysfunction and healthy controls

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