Development and validation of a modularized external fixator for generating standardized fracture healing micromotions in rats
Aims: To fully verify the reliability and reproducibility of an experimental method in generating standardized micromotion for the rat femur fracture model. Methods: A modularized experimental device has been developed that allows rat models to be used instead of large animal models, with the aim of...
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The British Editorial Society of Bone & Joint Surgery
2021
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oai:doaj.org-article:b91f7ed62a06443e9ff142664d18b23e2021-12-01T18:46:59ZDevelopment and validation of a modularized external fixator for generating standardized fracture healing micromotions in rats2046-375810.1302/2046-3758.1011.BJR-2021-0028.R1https://doaj.org/article/b91f7ed62a06443e9ff142664d18b23e2021-11-01T00:00:00Zhttps://online.boneandjoint.org.uk/doi/epdf/10.1302/2046-3758.1011.BJR-2021-0028.R1https://doaj.org/toc/2046-3758Aims: To fully verify the reliability and reproducibility of an experimental method in generating standardized micromotion for the rat femur fracture model. Methods: A modularized experimental device has been developed that allows rat models to be used instead of large animal models, with the aim of reducing systematic errors and time and money constraints on grouping. The bench test was used to determine the difference between the measured and set values of the micromotion produced by this device under different simulated loading weights. The displacement of the fixator under different loading conditions was measured by compression tests, which was used to simulate the unexpected micromotion caused by the rat’s ambulation. In vivo preliminary experiments with a small sample size were used to test the feasibility and effectiveness of the whole experimental scheme and surgical scheme. Results: The bench test showed that a weight loading < 500 g did not affect the operation of experimental device. The compression test demonstrated that the stiffness of the device was sufficient to keep the uncontrollable motion between fracture ends, resulting from the rat’s daily activities, within 1% strain. In vivo results on 15 rats prove that the device works reliably, without overburdening the experimental animals, and provides standardized micromotion reproductively at the fracture site according to the set parameters. Conclusion: Our device was able to investigate the effect of micromotion parameters on fracture healing by generating standardized micromotion to small animal models. Cite this article: Bone Joint Res 2021;10(11):714–722.Weichen QiXiaoreng FengTeng ZhangHao WuChristian FangFrankie LeungThe British Editorial Society of Bone & Joint Surgeryarticledesign for experimentssurgical instrumentsin vivomedical treatmentsmall animal modelbone fracturebone healing modelfracture healingrat modelsexternal fixatorsstrainfractured femorafracture sitesanimal modelsstiffnessfemorabody weightDiseases of the musculoskeletal systemRC925-935ENBone & Joint Research, Vol 10, Iss 11, Pp 714-722 (2021) |
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DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
design for experiments surgical instruments in vivo medical treatment small animal model bone fracture bone healing model fracture healing rat models external fixators strain fractured femora fracture sites animal models stiffness femora body weight Diseases of the musculoskeletal system RC925-935 |
| spellingShingle |
design for experiments surgical instruments in vivo medical treatment small animal model bone fracture bone healing model fracture healing rat models external fixators strain fractured femora fracture sites animal models stiffness femora body weight Diseases of the musculoskeletal system RC925-935 Weichen Qi Xiaoreng Feng Teng Zhang Hao Wu Christian Fang Frankie Leung Development and validation of a modularized external fixator for generating standardized fracture healing micromotions in rats |
| description |
Aims: To fully verify the reliability and reproducibility of an experimental method in generating standardized micromotion for the rat femur fracture model. Methods: A modularized experimental device has been developed that allows rat models to be used instead of large animal models, with the aim of reducing systematic errors and time and money constraints on grouping. The bench test was used to determine the difference between the measured and set values of the micromotion produced by this device under different simulated loading weights. The displacement of the fixator under different loading conditions was measured by compression tests, which was used to simulate the unexpected micromotion caused by the rat’s ambulation. In vivo preliminary experiments with a small sample size were used to test the feasibility and effectiveness of the whole experimental scheme and surgical scheme. Results: The bench test showed that a weight loading < 500 g did not affect the operation of experimental device. The compression test demonstrated that the stiffness of the device was sufficient to keep the uncontrollable motion between fracture ends, resulting from the rat’s daily activities, within 1% strain. In vivo results on 15 rats prove that the device works reliably, without overburdening the experimental animals, and provides standardized micromotion reproductively at the fracture site according to the set parameters. Conclusion: Our device was able to investigate the effect of micromotion parameters on fracture healing by generating standardized micromotion to small animal models. Cite this article: Bone Joint Res 2021;10(11):714–722. |
| format |
article |
| author |
Weichen Qi Xiaoreng Feng Teng Zhang Hao Wu Christian Fang Frankie Leung |
| author_facet |
Weichen Qi Xiaoreng Feng Teng Zhang Hao Wu Christian Fang Frankie Leung |
| author_sort |
Weichen Qi |
| title |
Development and validation of a modularized external fixator for generating standardized fracture healing micromotions in rats |
| title_short |
Development and validation of a modularized external fixator for generating standardized fracture healing micromotions in rats |
| title_full |
Development and validation of a modularized external fixator for generating standardized fracture healing micromotions in rats |
| title_fullStr |
Development and validation of a modularized external fixator for generating standardized fracture healing micromotions in rats |
| title_full_unstemmed |
Development and validation of a modularized external fixator for generating standardized fracture healing micromotions in rats |
| title_sort |
development and validation of a modularized external fixator for generating standardized fracture healing micromotions in rats |
| publisher |
The British Editorial Society of Bone & Joint Surgery |
| publishDate |
2021 |
| url |
https://doaj.org/article/b91f7ed62a06443e9ff142664d18b23e |
| work_keys_str_mv |
AT weichenqi developmentandvalidationofamodularizedexternalfixatorforgeneratingstandardizedfracturehealingmicromotionsinrats AT xiaorengfeng developmentandvalidationofamodularizedexternalfixatorforgeneratingstandardizedfracturehealingmicromotionsinrats AT tengzhang developmentandvalidationofamodularizedexternalfixatorforgeneratingstandardizedfracturehealingmicromotionsinrats AT haowu developmentandvalidationofamodularizedexternalfixatorforgeneratingstandardizedfracturehealingmicromotionsinrats AT christianfang developmentandvalidationofamodularizedexternalfixatorforgeneratingstandardizedfracturehealingmicromotionsinrats AT frankieleung developmentandvalidationofamodularizedexternalfixatorforgeneratingstandardizedfracturehealingmicromotionsinrats |
| _version_ |
1718404716691456000 |