Temperature, by Controlling Growth Rate, Regulates CRISPR-Cas Activity in <named-content content-type="genus-species">Pseudomonas aeruginosa</named-content>

ABSTRACT Clustered regularly interspaced short palindromic repeat (CRISPR)-associated (CRISPR-Cas) systems are adaptive defense systems that protect bacteria and archaea from invading genetic elements. In Pseudomonas aeruginosa, quorum sensing (QS) induces the CRISPR-Cas defense system at high cell...

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Autores principales:	Nina Molin Høyland-Kroghsbo, Katrina Arcelia Muñoz, Bonnie L. Bassler
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2018
Materias:	CRISPR phage Pseudomonas quorum sensing growth rate Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/b926fdf6b8094ab89adcd9387db50d15
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spelling	oai:doaj.org-article:b926fdf6b8094ab89adcd9387db50d152021-11-15T15:52:18ZTemperature, by Controlling Growth Rate, Regulates CRISPR-Cas Activity in <named-content content-type="genus-species">Pseudomonas aeruginosa</named-content>10.1128/mBio.02184-182150-7511https://doaj.org/article/b926fdf6b8094ab89adcd9387db50d152018-12-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mBio.02184-18https://doaj.org/toc/2150-7511ABSTRACT Clustered regularly interspaced short palindromic repeat (CRISPR)-associated (CRISPR-Cas) systems are adaptive defense systems that protect bacteria and archaea from invading genetic elements. In Pseudomonas aeruginosa, quorum sensing (QS) induces the CRISPR-Cas defense system at high cell density when the risk of bacteriophage infection is high. Here, we show that another cue, temperature, modulates P. aeruginosa CRISPR-Cas. Increased CRISPR adaptation occurs at environmental (i.e., low) temperatures compared to that at body (i.e., high) temperature. This increase is a consequence of the accumulation of CRISPR-Cas complexes, coupled with reduced P. aeruginosa growth rate at the lower temperature, the latter of which provides additional time prior to cell division for CRISPR-Cas to patrol the cell and successfully eliminate and/or acquire immunity to foreign DNA. Analyses of a QS mutant and synthetic QS compounds show that the QS and temperature cues act synergistically. The diversity and level of phage encountered by P. aeruginosa in the environment exceed that in the human body, presumably warranting increased reliance on CRISPR-Cas at environmental temperatures. IMPORTANCE P. aeruginosa is a soil dwelling bacterium and a plant pathogen, and it also causes life-threatening infections in humans. Thus, P. aeruginosa thrives in diverse environments and over a broad range of temperatures. Some P. aeruginosa strains rely on the CRISPR-Cas adaptive immune system as a phage defense mechanism. Our discovery that low temperatures increase CRISPR adaptation suggests that the rarely occurring but crucial naive adaptation events may take place predominantly under conditions of slow growth, e.g., during the bacterium’s soil dwelling existence and during slow growth in biofilms.Nina Molin Høyland-KroghsboKatrina Arcelia MuñozBonnie L. BasslerAmerican Society for MicrobiologyarticleCRISPRphagePseudomonasquorum sensinggrowth rateMicrobiologyQR1-502ENmBio, Vol 9, Iss 6 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	CRISPR phage Pseudomonas quorum sensing growth rate Microbiology QR1-502
spellingShingle	CRISPR phage Pseudomonas quorum sensing growth rate Microbiology QR1-502 Nina Molin Høyland-Kroghsbo Katrina Arcelia Muñoz Bonnie L. Bassler Temperature, by Controlling Growth Rate, Regulates CRISPR-Cas Activity in <named-content content-type="genus-species">Pseudomonas aeruginosa</named-content>
description	ABSTRACT Clustered regularly interspaced short palindromic repeat (CRISPR)-associated (CRISPR-Cas) systems are adaptive defense systems that protect bacteria and archaea from invading genetic elements. In Pseudomonas aeruginosa, quorum sensing (QS) induces the CRISPR-Cas defense system at high cell density when the risk of bacteriophage infection is high. Here, we show that another cue, temperature, modulates P. aeruginosa CRISPR-Cas. Increased CRISPR adaptation occurs at environmental (i.e., low) temperatures compared to that at body (i.e., high) temperature. This increase is a consequence of the accumulation of CRISPR-Cas complexes, coupled with reduced P. aeruginosa growth rate at the lower temperature, the latter of which provides additional time prior to cell division for CRISPR-Cas to patrol the cell and successfully eliminate and/or acquire immunity to foreign DNA. Analyses of a QS mutant and synthetic QS compounds show that the QS and temperature cues act synergistically. The diversity and level of phage encountered by P. aeruginosa in the environment exceed that in the human body, presumably warranting increased reliance on CRISPR-Cas at environmental temperatures. IMPORTANCE P. aeruginosa is a soil dwelling bacterium and a plant pathogen, and it also causes life-threatening infections in humans. Thus, P. aeruginosa thrives in diverse environments and over a broad range of temperatures. Some P. aeruginosa strains rely on the CRISPR-Cas adaptive immune system as a phage defense mechanism. Our discovery that low temperatures increase CRISPR adaptation suggests that the rarely occurring but crucial naive adaptation events may take place predominantly under conditions of slow growth, e.g., during the bacterium’s soil dwelling existence and during slow growth in biofilms.
format	article
author	Nina Molin Høyland-Kroghsbo Katrina Arcelia Muñoz Bonnie L. Bassler
author_facet	Nina Molin Høyland-Kroghsbo Katrina Arcelia Muñoz Bonnie L. Bassler
author_sort	Nina Molin Høyland-Kroghsbo
title	Temperature, by Controlling Growth Rate, Regulates CRISPR-Cas Activity in <named-content content-type="genus-species">Pseudomonas aeruginosa</named-content>
title_short	Temperature, by Controlling Growth Rate, Regulates CRISPR-Cas Activity in <named-content content-type="genus-species">Pseudomonas aeruginosa</named-content>
title_full	Temperature, by Controlling Growth Rate, Regulates CRISPR-Cas Activity in <named-content content-type="genus-species">Pseudomonas aeruginosa</named-content>
title_fullStr	Temperature, by Controlling Growth Rate, Regulates CRISPR-Cas Activity in <named-content content-type="genus-species">Pseudomonas aeruginosa</named-content>
title_full_unstemmed	Temperature, by Controlling Growth Rate, Regulates CRISPR-Cas Activity in <named-content content-type="genus-species">Pseudomonas aeruginosa</named-content>
title_sort	temperature, by controlling growth rate, regulates crispr-cas activity in <named-content content-type="genus-species">pseudomonas aeruginosa</named-content>
publisher	American Society for Microbiology
publishDate	2018
url	https://doaj.org/article/b926fdf6b8094ab89adcd9387db50d15
work_keys_str_mv	AT ninamolinhøylandkroghsbo temperaturebycontrollinggrowthrateregulatescrisprcasactivityinnamedcontentcontenttypegenusspeciespseudomonasaeruginosanamedcontent AT katrinaarceliamunoz temperaturebycontrollinggrowthrateregulatescrisprcasactivityinnamedcontentcontenttypegenusspeciespseudomonasaeruginosanamedcontent AT bonnielbassler temperaturebycontrollinggrowthrateregulatescrisprcasactivityinnamedcontentcontenttypegenusspeciespseudomonasaeruginosanamedcontent
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Temperature, by Controlling Growth Rate, Regulates CRISPR-Cas Activity in <named-content content-type="genus-species">Pseudomonas aeruginosa</named-content>

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