Tissue storage affects lipidome profiling in comparison to in vivo microsampling approach

Abstract Low-invasive in vivo solid-phase microextraction (SPME) was used to investigate the lipid profiles of muscle tissue of living fish. Briefly, mixed mode SPME fibers were inserted into the muscle for 20 min extraction, and then the fibers were desorbed in an optimal mixture of solvents. The o...

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Autores principales:	Anna Roszkowska, Miao Yu, Vincent Bessonneau, Leslie Bragg, Mark Servos, Janusz Pawliszyn
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b929cd0fe14c447f8692b54e6e94e8a4
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spelling	oai:doaj.org-article:b929cd0fe14c447f8692b54e6e94e8a42021-12-02T16:08:25ZTissue storage affects lipidome profiling in comparison to in vivo microsampling approach10.1038/s41598-018-25428-22045-2322https://doaj.org/article/b929cd0fe14c447f8692b54e6e94e8a42018-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-25428-2https://doaj.org/toc/2045-2322Abstract Low-invasive in vivo solid-phase microextraction (SPME) was used to investigate the lipid profiles of muscle tissue of living fish. Briefly, mixed mode SPME fibers were inserted into the muscle for 20 min extraction, and then the fibers were desorbed in an optimal mixture of solvents. The obtained lipid profile was then compared and contrasted to that obtained with employment of ex vivo SPME and solid-liquid extraction (SLE) from fish muscle tissue belonging to the same group of fish, following a one-year storage period. Ex vivo SPME analysis of stored muscle samples revealed 10-fold decrease in the number of detected molecular features in comparison to in vivo study. Moreover, in vivo microsampling enabled the identification of different classes of bioactive lipids, including fatty acyls, not present in the lipid profile obtained through ex vivo SPME and SLE, suggesting the alterations occurring in the unbound lipid fraction of the system under study during the storage and also indicating the advantage of the in vivo extraction approach.Anna RoszkowskaMiao YuVincent BessonneauLeslie BraggMark ServosJanusz PawliszynNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-10 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Anna Roszkowska Miao Yu Vincent Bessonneau Leslie Bragg Mark Servos Janusz Pawliszyn Tissue storage affects lipidome profiling in comparison to in vivo microsampling approach
description	Abstract Low-invasive in vivo solid-phase microextraction (SPME) was used to investigate the lipid profiles of muscle tissue of living fish. Briefly, mixed mode SPME fibers were inserted into the muscle for 20 min extraction, and then the fibers were desorbed in an optimal mixture of solvents. The obtained lipid profile was then compared and contrasted to that obtained with employment of ex vivo SPME and solid-liquid extraction (SLE) from fish muscle tissue belonging to the same group of fish, following a one-year storage period. Ex vivo SPME analysis of stored muscle samples revealed 10-fold decrease in the number of detected molecular features in comparison to in vivo study. Moreover, in vivo microsampling enabled the identification of different classes of bioactive lipids, including fatty acyls, not present in the lipid profile obtained through ex vivo SPME and SLE, suggesting the alterations occurring in the unbound lipid fraction of the system under study during the storage and also indicating the advantage of the in vivo extraction approach.
format	article
author	Anna Roszkowska Miao Yu Vincent Bessonneau Leslie Bragg Mark Servos Janusz Pawliszyn
author_facet	Anna Roszkowska Miao Yu Vincent Bessonneau Leslie Bragg Mark Servos Janusz Pawliszyn
author_sort	Anna Roszkowska
title	Tissue storage affects lipidome profiling in comparison to in vivo microsampling approach
title_short	Tissue storage affects lipidome profiling in comparison to in vivo microsampling approach
title_full	Tissue storage affects lipidome profiling in comparison to in vivo microsampling approach
title_fullStr	Tissue storage affects lipidome profiling in comparison to in vivo microsampling approach
title_full_unstemmed	Tissue storage affects lipidome profiling in comparison to in vivo microsampling approach
title_sort	tissue storage affects lipidome profiling in comparison to in vivo microsampling approach
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/b929cd0fe14c447f8692b54e6e94e8a4
work_keys_str_mv	AT annaroszkowska tissuestorageaffectslipidomeprofilingincomparisontoinvivomicrosamplingapproach AT miaoyu tissuestorageaffectslipidomeprofilingincomparisontoinvivomicrosamplingapproach AT vincentbessonneau tissuestorageaffectslipidomeprofilingincomparisontoinvivomicrosamplingapproach AT lesliebragg tissuestorageaffectslipidomeprofilingincomparisontoinvivomicrosamplingapproach AT markservos tissuestorageaffectslipidomeprofilingincomparisontoinvivomicrosamplingapproach AT januszpawliszyn tissuestorageaffectslipidomeprofilingincomparisontoinvivomicrosamplingapproach
_version_	1718384491497521152

Tissue storage affects lipidome profiling in comparison to in vivo microsampling approach

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